NMR studies on the conformation, stability and dynamics of alamethicin in methanol

SummaryPhysico-chemical properties of recombinant desulphatohirudin expressed in yeast (CIBA GEIGY code No. CGP 39393) were reinvestigated. As previously reported for natural hirudin, the recombinant molecule exhibited abnormal behaviour by gel filtration with an apparent molecular weight greater than that based on the primary structure. However, molecular weight estimation by SDS gel electrophoresis, FAB-mass spectrometry and Photon Correlation Spectroscopy were in agreement with the theoretical molecular weight, with little suggestion of dimer or aggregate formation. Circular dichroism studies of the recombinant molecule show similar spectra at different pH values but are markedly different from that reported by Konno et al. (13) for a natural hirudin-variant. Our CD studies indicate the presence of about 60% beta sheet and the absence of alpha helix in the secondary structure of recombinant hirudin, in agreement with the conformation determined by NMR studies (17)

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NMR studies of alkali intercalted carbon nanotubes

MRS Proceedings ◽

10.1557/proc-772-m3.4 ◽

2003 ◽

Vol 772 ◽

Cited By ~ 1

Author(s):

M. Schmid ◽

C. Goze-Bac ◽

M. Mehring ◽

S. Roth ◽

P. Bernier

Keyword(s):

Carbon Nanotubes ◽

Lattice Relaxation ◽

Spin Lattice Relaxation ◽

Energy Storage Devices ◽

Nmr Studies ◽

Storage Devices ◽

Spin Lattice ◽

Relaxation Measurements ◽

Resonance Spin ◽

Walled Carbon Nanotubes

AbstractLithium intercalted carbon nanotubes have attracted considerable interest as perspective components for energy storage devices. We performed 13C Nuclear Magnetic Resonance spin lattice relaxation measurements in a temperature range from 4 K up to 300 on alkali intercalated Single Walled Carbon Nanotubes in order to investigate the modifications of the electronic properties. The density of states at the Fermi level were determined for pristine, lithium and cesium intercalated carbon nanotubes and are discussed in terms of intercalation and charge transfer effects.

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NMR studies and multi-scale modeling of the water and ionic mobilities within the porous networks limited by clay particles

European Journal of Control ◽

10.3166/acsm.30.263-276 ◽

2005 ◽

Vol 30 (3) ◽

pp. 263-276 ◽

Cited By ~ 3

Author(s):

Patrice Porion ◽

Alfred Delville

Keyword(s):

Nmr Studies ◽

Clay Particles ◽

Multi Scale ◽

Porous Networks ◽

Scale Modeling ◽

Ionic Mobilities ◽

Multi Scale Modeling

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Faculty Opinions recommendation of Quantitative NMR studies of high molecular weight proteins: application to domain orientation and ligand binding in the 723 residue enzyme malate synthase G.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1013349.190158 ◽

2003 ◽

Author(s):

H Jane Dyson

Keyword(s):

Molecular Weight ◽

Ligand Binding ◽

High Molecular Weight ◽

Malate Synthase ◽

Nmr Studies ◽

Quantitative Nmr ◽

Domain Orientation ◽

Malate Synthase G ◽

High Molecular Weight Proteins

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Faculty Opinions recommendation of Production of membrane proteins for NMR studies using the condensed single protein (cSPP) production system.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1443959.922057 ◽

2010 ◽

Author(s):

Linda Ball ◽

Victoria Ann Higman

Keyword(s):

Membrane Proteins ◽

Production System ◽

Nmr Studies ◽

Single Protein

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NMR Studies of the Inclusion Complexes Between Ezetimibe and Cyclodextrins

Revista de Chimie ◽

10.37358/rc.18.7.6427 ◽

2018 ◽

Vol 69 (7) ◽

pp. 1838-1841

Author(s):

Hajnal Kelemen ◽

Angella Csillag ◽

Bela Noszal ◽

Gabor Orgovan

Keyword(s):

Inclusion Complex ◽

Inclusion Complexes ◽

Water Solubility ◽

Solution Nmr ◽

Spectroscopic Techniques ◽

Nmr Studies ◽

The Poor ◽

Poor Water Solubility

Ezetimibe, the antihyperlipidemic drug of poor bioavailability was complexed with native and derivatized cyclodextrins.The complexes were characterized in terms stability, stoichiometry and structure using various 1D and 2D solution NMR spectroscopic techniques. The complexes were found to be of moderate stability (logK[3). The least stable inclusion complex is formed with b-cyclodextrin, while the ezetimibe-methylated-b--cyclodextrin has a 7-fold higher stability. The results can be useful to improve the poor water-solubility and the concomitant bioavailability of ezetimibe.

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