A novel biocatalytic approach to acetylation of 1-β-d-arabinofuranosylcytosine by Aspergillus oryzae whole cell in organic solvents

2011 ◽  
Vol 93 (1) ◽  
pp. 143-150 ◽  
Author(s):  
Xiao-Feng Li ◽  
Zhen Zhu ◽  
Guang-Lei Zhao ◽  
Yi-Gang Yu ◽  
Fu-Rao Lai ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Aspergillus Oryzae ◽  
Whole Cell

scholarly journals Low-Level Organic Solvents Improve Multienzyme Whole-Cell Catalytic Synthesis of Myricetin-7-O-Glucuronide

Catalysts ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 970 ◽  
Author(s):  
Yan Yang ◽  
Min-Zhi Liu ◽  
Yun-Song Cao ◽  
Chang-Kun Li ◽  
Wei Wang
Keyword(s):  
Cell Membrane ◽  
Organic Solvents ◽  
Membrane Integrity ◽  
Industrial Applications ◽  
Coli Strain ◽  
Whole Cell ◽  
Cell Membrane Integrity ◽  
E Coli ◽  
Low Level ◽  
Whole Cell Catalysis

Multienzyme whole-cell biocatalysts are preferred in industrial applications, and two major concerns regarding the use of these biocatalysts, cell viability and cell membrane integrity, must be addressed. In this work, the transformation of myricetin to myricetin-7-O-glucuronide catalyzed by an engineered Escherichia coli strain was taken as the model reaction to examine the impacts of low-level organic solvents on whole-cell biocatalysis. Low-level organic solvents (2%, v/v) showed a significant increase (roughly 13-fold) in myricetin-7-O-glucuronide yields. No obvious compromises of cellular viability and integrity were observed by a flow cytometry assay or in the determination of extracellular protein leakage, suggesting the addition of low-level organic solvents accommodates whole E. coli cells. Furthermore, a scaled-up reaction was conducted to test the capability and efficiency of whole-cell catalysis in the presence of organic solvents. This study presents a promising and simple means to enhance the productivity of multienzyme whole-cell catalysis without losing the barrier functions of the cell membrane.

Utilization of hydrophobic bacterium Rhodococcus opacus B-4 as whole-cell catalyst in anhydrous organic solvents

2007 ◽  
Vol 74 (4) ◽  
pp. 761-767 ◽  
Author(s):  
Shiho Yamashita ◽  
Masafumi Satoi ◽  
Yoshihiro Iwasa ◽  
Kohsuke Honda ◽  
Yuka Sameshima ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Rhodococcus Opacus ◽  
Whole Cell

scholarly journals Influence of Organic Solvents on Catalytic Behaviors and Cell Morphology of Whole-Cell Biocatalysts for Synthesis of 5′-Arabinocytosine Laurate

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104847 ◽  
Author(s):  
Meiyan Yang ◽  
Hui Wu ◽  
Yan Lian ◽  
Xiaofeng Li ◽  
Furao Lai ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Cell Morphology ◽  
Whole Cell

Conversion of Chlamydomonas sp. JSC4 lipids to biodiesel using Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell as biocatalyst

2017 ◽  
Vol 28 ◽  
pp. 16-23 ◽  
Author(s):  
Jerome Amoah ◽  
Shih-Hsin Ho ◽  
Shinji Hama ◽  
Ayumi Yoshida ◽  
Akihito Nakanishi ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Whole Cell ◽  
Fusarium Heterosporum ◽  
Chlamydomonas Sp

Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from Candida antarctica

2007 ◽  
Vol 75 (2) ◽  
pp. 387-395 ◽  
Author(s):  
Sriappareddy Tamalampudi ◽  
MD Mahabubur Rahman Talukder ◽  
Shinji Hama ◽  
Takanori Tanino ◽  
Yuya Suzuki ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Candida Antarctica ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Encoding Gene

A novel and highly regioselective biocatalytic approach to acetylation of helicid by using whole-cell biocatalysts in organic solvents

2019 ◽  
Vol 128 ◽  
pp. 105707 ◽  
Author(s):  
Rong-Ling Yang ◽  
Xiang-Jie Zhao ◽  
Ting-Ting Wu ◽  
Muhammad Bilal ◽  
Zhao-Yu Wang ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Whole Cell
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