Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization

2012 ◽  
Vol 97 (7) ◽  
pp. 2951-2959 ◽  
Author(s):  
Yukiko Shinozaki ◽  
Tomotake Morita ◽  
Xiao-hong Cao ◽  
Shigenobu Yoshida ◽  
Motoo Koitabashi ◽  
...  
Keyword(s):  
Biodegradable Plastic ◽  
Degrading Enzyme ◽  
Pseudozyma Antarctica

scholarly journals Disruption of protease A and B orthologous genes in the basidiomycetous yeast Pseudozyma antarctica GB-4(0) yields a stable extracellular biodegradable plastic-degrading enzyme

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0247462
Author(s):  
Natsuki Omae ◽  
Yuka Sameshima-Yamashita ◽  
Kazunori Ushimaru ◽  
Hideaki Koike ◽  
Hiroko Kitamoto ◽  
...  
Keyword(s):  
Large Scale ◽  
Amino Acid Sequences ◽  
Biodegradable Plastic ◽  
Orthologous Genes ◽  
Degrading Enzyme ◽  
Pseudozyma Antarctica ◽  
Overexpression Strain ◽  
Genes Encoding ◽  
Large Scale Cultivation ◽  
And Storage

The yeast Pseudozyma antarctica (currently designated Moesziomyces antarcticus) secretes a xylose-induced biodegradable plastic-degrading enzyme (PaE). To suppress degradation of PaE during production and storage, we targeted the inhibition of proteolytic enzyme activity in P. antarctica. Proteases A and B act as upper regulators in the proteolytic network of the model yeast, Saccharomyces cerevisiae. We searched for orthologous genes encoding proteases A and B in the genome of P. antarctica GB-4(0) based on the predicted amino acid sequences. We found two gene candidates, PaPRO1 and PaPRO2, with conserved catalytically important domains and signal peptides indicative of vacuolar protease function. We then prepared gene-deletion mutants of strain GB-4(0), ΔPaPRO1 and ΔPaPRO2, and evaluated PaE stability in culture by immunoblotting analysis. Both mutants exhibited sufficient production of PaE without degradation fragments, while the parent strain exhibited the degradation fragments. Therefore, we concluded that the protease A and B orthologous genes are related to the degradation of PaE. To produce a large quantity of PaE, we made a PaPRO2 deletion mutant of a PaE-overexpression strain named XG8 by introducing a PaE high-production cassette into the strain GB-4(0). The ΔPaPRO2 mutant of XG8 was able to produce PaE without the degradation fragments during large-scale cultivation in a 3-L jar fermenter for 3 days at 30°C. After terminating the agitation, the PaE activity in the XG8 ΔPaPRO2 mutant culture was maintained for the subsequent 48 h incubation at 25°C regardless of remaining cells, while activity in the XG8 control was reduced to 55.1%. The gene-deleted mutants will be useful for the development of industrial processes of PaE production and storage.

Production of a biodegradable plastic-degrading enzyme from cheese whey by the phyllosphere yeast Pseudozyma antarctica GB-4(1)W

2014 ◽  
Vol 118 (2) ◽  
pp. 183-187 ◽  
Author(s):  
Takashi Watanabe ◽  
Yukiko Shinozaki ◽  
Ken Suzuki ◽  
Motoo Koitabashi ◽  
Shigenobu Yoshida ◽  
...  
Keyword(s):  
Cheese Whey ◽  
Biodegradable Plastic ◽  
Degrading Enzyme ◽  
Pseudozyma Antarctica

A UV-induced mutant of Cryptococcus flavus GB-1 with increased production of a biodegradable plastic-degrading enzyme

2015 ◽  
Vol 50 (11) ◽  
pp. 1718-1724 ◽  
Author(s):  
Takashi Watanabe ◽  
Ken Suzuki ◽  
Yukiko Shinozaki ◽  
Tohru Yarimizu ◽  
Shigenobu Yoshida ◽  
...  
Keyword(s):  
Biodegradable Plastic ◽  
Degrading Enzyme ◽  
Induced Mutant

scholarly journals Enhancement of Biodegradable Plastic-degrading Enzyme Production from Paraphoma-like Fungus, Strain B47-9

2016 ◽  
Vol 65 (3) ◽  
pp. 257-262 ◽  
Author(s):  
Yuka Sameshima-Yamashita ◽  
Motoo Koitabashi ◽  
Wataru Tsuchiya ◽  
Ken Suzuki ◽  
Takashi Watanabe ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Biodegradable Plastic ◽  
Degrading Enzyme

Purification and characterization of a biodegradable plastic-degrading enzyme from Aspergillus oryzae

2005 ◽  
Vol 67 (6) ◽  
pp. 778-788 ◽  
Author(s):  
Hiroshi Maeda ◽  
Youhei Yamagata ◽  
Keietsu Abe ◽  
Fumihiko Hasegawa ◽  
Masayuki Machida ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Biodegradable Plastic ◽  
Purification And Characterization ◽  
Degrading Enzyme

scholarly journals Draft Genome Sequence of the Fungus Paraphoma sp. B47-9, a Producer of a Biodegradable Plastic–Degrading Enzyme

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Yuka Sameshima-Yamashita ◽  
Hideaki Koike ◽  
Motoo Koitabashi ◽  
Azusa Saika ◽  
Tomotake Morita ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Genome Assembly ◽  
Draft Genome ◽  
Gc Content ◽  
Biodegradable Plastic ◽  
Draft Genome Sequence ◽  
Degrading Enzyme ◽  
Draft Genome Assembly

Paraphoma sp. B47-9 is a producer of a biodegradable plastic–degrading enzyme. Here, we report the draft genome sequence of this strain. The draft genome assembly has a size of 39.3 Mb with a GC content of 52.4% and consists of 185 scaffolds.

Purification, characterization, and cloning of the gene for a biodegradable plastic-degrading enzyme from Paraphoma-related fungal strain B47-9

2014 ◽  
Vol 98 (10) ◽  
pp. 4457-4465 ◽  
Author(s):  
Ken Suzuki ◽  
Masako Tsujimoto Noguchi ◽  
Yukiko Shinozaki ◽  
Motoo Koitabashi ◽  
Yuka Sameshima-Yamashita ◽  
...  
Keyword(s):  
Fungal Strain ◽  
Biodegradable Plastic ◽  
Degrading Enzyme
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