Degradation profiles of biodegradable plastic films by biodegradable plastic-degrading enzymes from the yeast Pseudozyma antarctica and the fungus Paraphoma sp. B47-9

Abstract Phylloplane yeast genera Pseudozyma and Cryptococcus secrete biodegradable plastic (BP)-degrading enzymes, termed cutinase-like enzymes (CLEs). Although, CLEs contain highly conserved catalytic sites, the whole protein exhibits ≤ 30% amino acid sequence homology with cutinase. In this study, we analyzed whether CLEs exhibit cutinase activity. Seventeen Cryptococcus magnus strains, which degrade BP at 15 °C, were isolated from leaves, and identified the DNA sequence of the CLE in one of the strains. Cutin was prepared from tomato leaves and treated with CLEs from three Cryptococcus species (C. magnus, Cryptococcus flavus, and Cryptococcus laurentii) and Pseudozyma antarctia (PaE). A typical cutin monomer, 10,16-dihydroxyhexadecanoic acid, was detected in extracts of the reaction solution via gas chromatography-mass spectrometry, showing that cutin was indeed degraded by CLEs. In addition to the aforementioned monomer, separation analysis via thin-layer chromatography detected high-molecular-weight products resulting from the breakdown of cutin by PaE, indicating that PaE acts as an endo-type enzyme.

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Biofilm Formation and Degradation of Commercially Available Biodegradable Plastic Films by Bacterial Consortiums in Freshwater Environments

Microbes and Environments ◽

10.1264/jsme2.me18033 ◽

2018 ◽

Vol 33 (3) ◽

pp. 332-335 ◽

Cited By ~ 21

Author(s):

Tomohiro Morohoshi ◽

Taishiro Oi ◽

Haruna Aiso ◽

Tomohiro Suzuki ◽

Tetsuo Okura ◽

...

Keyword(s):

Biofilm Formation ◽

Biodegradable Plastic ◽

Plastic Films ◽

Freshwater Environments

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COMPOUNDING AND PROCESSING HYDRO-BIODEGRADABLE PLASTIC FILMS FOR PLASTIC WASTE REDUCTION. PART I: PROCESSING CONDITIONS AND ENVIRONMENTAL PERFORMANCE AGAINST PLASTIC SOLID WASTE

Waste Management and the Environment X ◽

10.2495/wm200091 ◽

2020 ◽

Author(s):

HAJAR JAWAD KARAM ◽

MASUMAH AL-QASSIMI ◽

MAJED HAMEED AL-WADI ◽

AISHA ABDULLAH AL-ROWAIH ◽

ABDUL SALAM AL-HAZZA’A ◽

...

Keyword(s):

Solid Waste ◽

Environmental Performance ◽

Plastic Waste ◽

Waste Reduction ◽

Biodegradable Plastic ◽

Processing Conditions ◽

Plastic Films

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Synthesis and Characterization of Biodegradable Blend based on LDPE/cassava stem nanofiber cellulose

Journal of Chemical Process Engineering ◽

10.33536/jcpe.v6i1.732 ◽

2021 ◽

Vol 6 (1) ◽

pp. 8-17

Author(s):

L Lismeri ◽

N Herdiana ◽

D Kameswara ◽

P S Anungputri ◽

Y Darni ◽

...

Keyword(s):

Organic Material ◽

Water Resistance ◽

Environmental Problem ◽

Biodegradable Plastic ◽

Synthesis And Characterization ◽

Plastic Films ◽

Degradation Test ◽

Cassava Stem ◽

Degradation Time

Conventional plastic becomes trend topic due to its long degradation time and needs attention related to environmental problem. One type of plastic that is difficult to be degraded is LDPE. Some of the efforts done is to synthesize plastics with organic material so that it becomes biodegradable plastic. Cellulose is an organic material that is abundant in nature and can be used as a filler. This research aims to synthesis the biodegradable plastic films composted by nanocellulose – LDPE. Mechanical (UTM), water resistance and degradation test has been done. The properties of the biodegradable blend still meet the commercial LDPE standart. Even though the biocomposite based on LDPE-nanofiber cellulose can not totally degradable but it is can be used as a solution to reduce the degradation time of a plastic waste.

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