Novel expression system for Corynebacterium acetoacidophilum and Escherichia coli based on the T7 RNA polymerase-dependent promoter

2013 ◽  
Vol 97 (17) ◽  
pp. 7755-7766 ◽  
Author(s):  
Md. Javed Equbal ◽  
Preeti Srivastava ◽  
Gopal Prasad Agarwal ◽  
Jahar Kanti Deb
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Expression System ◽  
T7 Rna Polymerase

scholarly journals Stringent control in Escherichia coli applies also to transcription by T7 RNA polymerase.

1987 ◽  
Vol 262 (9) ◽  
pp. 3940-3943
Author(s):  
M. Yamagishi ◽  
J.R. Cole ◽  
M. Nomura ◽  
F.W. Studier ◽  
J.J. Dunn
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
T7 Rna Polymerase ◽  
Stringent Control

High-level transcription of large gene regions: a novel T7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus

2005 ◽  
Vol 33 (1) ◽  
pp. 56-58 ◽  
Author(s):  
T. Drepper ◽  
S. Arvani ◽  
F. Rosenau ◽  
S. Wilhelm ◽  
K.-E. Jaeger
Keyword(s):  
Rna Polymerase ◽  
Rhodobacter Capsulatus ◽  
Expression System ◽  
Active Form ◽  
T7 Rna Polymerase ◽  
High Level Synthesis ◽  
Phototrophic Bacterium ◽  
Large Gene ◽  
High Level ◽  
Complex Enzymes

High-level synthesis of complex enzymes like bacterial [NiFe] hydrogenases, in general, requires an expression system that allows concerted expression of a large number of genes. So far, it has not been possible to overproduce a hydrogenase in a stable and active form by using a customary expression system. Therefore we started to establish a new, T7-based expression system in the phototrophic bacterium Rhodobacter capsulatus. The beneficial properties of this bacterial host in combination with the unique capacity of T7 RNA polymerase to synthesize long transcripts will allow the high-level synthesis and assembly of active hydrogenase as well as other complex enzymes in the near future.

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