Assessment of the horizontal transfer of functional genes as a suitable approach for evaluation of the bioremediation potential of petroleum-contaminated sites: a mini-review

ABSTRACTHorizontal transfer of the chlorothalonil hydrolytic dehalogenase gene (chd) is proposed based on the high conservation of thechdgene and its close association with a novel insertion sequence, ISOcsp1, in 16 isolated chlorothalonil-dechlorinating strains belonging to eight different genera. The ecological role of horizontal gene transfer is assumed to facilitate bacterial adaptation to chlorothalonil-contaminated sites, through detoxification of chlorothalonil to less toxic 2,4,5-trichloro-6-hydroxybenzene-1,3-dicarbonitrile.

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Relationships between the Abundance and Expression of Functional Genes from Vinyl Chloride (VC)-Degrading Bacteria and Geochemical Parameters at VC-Contaminated Sites

Environmental Science & Technology ◽

10.1021/acs.est.7b03521 ◽

2017 ◽

Vol 51 (21) ◽

pp. 12164-12174 ◽

Cited By ~ 12

Author(s):

Yi Liang ◽

Xikun Liu ◽

Michael A. Singletary ◽

Kai Wang ◽

Timothy E. Mattes

Keyword(s):

Vinyl Chloride ◽

Contaminated Sites ◽

Functional Genes ◽

Geochemical Parameters ◽

Degrading Bacteria

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Contrasting microbial functional genes in two distinct saline-alkali and slightly acidic oil-contaminated sites

The Science of The Total Environment ◽

10.1016/j.scitotenv.2014.04.032 ◽

2014 ◽

Vol 487 ◽

pp. 272-278 ◽

Cited By ~ 12

Author(s):

Yuting Liang ◽

Huihui Zhao ◽

Xu Zhang ◽

Jizhong Zhou ◽

Guanghe Li

Keyword(s):

Contaminated Sites ◽

Functional Genes

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HCH Removal in a Biochar-Amended Biofilter

Water ◽

10.3390/w13233396 ◽

2021 ◽

Vol 13 (23) ◽

pp. 3396

Author(s):

Aday Amirbekov ◽

Aigerim Mamirova ◽

Alena Sevcu ◽

Roman Spanek ◽

Pavel Hrabak

Keyword(s):

Transformation Products ◽

Drainage Water ◽

Contaminated Sites ◽

Functional Genes ◽

High Effectiveness ◽

Hch Isomers ◽

Temporary Water ◽

Treatment Technologies ◽

Sulfur Oxidizing

This study evaluated the efficiency of two biofilter systems, with and without biochar chambers installed, at degrading and removing HCH and its isomers in natural drainage water. The biochar biofilter proved to be 96% efficient at cleaning HCH and its transformation products from drainage water, a significant improvement over classic biofilter that remove, on average, 68% of HCH. Although iron- and sulfur-oxidizing bacteria, such as Gallionella and Sulfuricurvum, were dominant in the biochar bed outflows, they were absent in sediments, which were rich in Simplicispira, Rhodoluna, Rhodoferax, and Flavobacterium. The presence of functional genes involved in the biodegradation of HCH isomers and their byproducts was confirmed in both systems. The high effectiveness of the biochar biofilter displayed in this study should further encourage the use of biochar in water treatment solutions, e.g., for temporary water purification installations during the construction of other long-term wastewater treatment technologies, or even as final solutions at contaminated sites.

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