Isolation and characterization of a thermophilic bacillus strain, that degrades phenol and cresols as sole carbon source at 70 °C

1996 ◽  
Vol 46 (5-6) ◽  
pp. 593-596 ◽  
Author(s):  
A. Mutzel ◽  
U. M. Reinscheid ◽  
G. Antranikian ◽  
R. Müller
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Thermophilic Bacillus ◽  
Bacillus Strain ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of a Novel p,p’-DDT-Degrading Bacterium: Aeromonas sp. Strain MY1 from Agricultural Soil

2020 ◽  
pp. 12-22
Author(s):  
Y. Murtala ◽  
B. C. Nwanguma ◽  
L. U. S. Ezeanyika
Keyword(s):  
Carbon Source ◽  
16S Rrna ◽  
Agricultural Soil ◽  
Sole Carbon Source ◽  
Phylogenetic Analyses ◽  
Inhibitory Effect ◽  
Aerobic Conditions ◽  
Degrading Bacterium ◽  
Isolation And Characterization

Background: Despite the banned on the use of dichlorodiphenyltrichloroethane (DDT) and other Persistent Organic Pollutants (POPs) by the Stockholm Convention for their toxicity, emerging shreds of evidence have indicated that DDT is, however, still in use in developing countries. This might increase the global burden of DDT contamination and its hazardous effects. Aim: This study focused on the isolation and characterization of p,p’-DDT-degrading bacterium from a tropical agricultural soil. Methodology: Standard isolation procedure was used for the screening and isolation of the strain. The 16S rRNA and phylogenetic analyses were used to identify the isolate and established protocols were followed to characterize the strain. Results: A new strain belonging to the genus Aeromonas was isolated from agricultural soil using minimal salt-p,p’-DDT enrichment medium. The 16S rRNA sequencing was used to identify the strain and the partial sequence was deposited in the NCBI GenBank as Aeromonas sp. Strain MY1. This mesophilic isolate was capable of utilizing up to 50 mgL-1 of p,p’-DDT as the sole carbon source at an optimum pH of 7.5 and optimum temperature of 35 °C within 120 h under aerobic conditions. Fe2+ (0.2 mgL-1) demonstrated a stimulatory effect on the p,p’-DDT degradation capacity by the strain MY1. However, Zn, Cu, Pb, Hg, Ag and Cr ions have demonstrated various patterns of inhibitory effect on the p,p’-DDT degradation capacity of the isolate at 0.2 mgL-1. The strain MY1 could be a promising candidate for the bioremediation of p,p’-DDT contaminant. Conclusion: Aeromonas sp. strain MY1 was capable of utilizing p,p’-DDT as a sole carbon source under aerobic conditions. The utilization capacity of the strain was influenced by some heavy metals. Fe was found to enhance the p,p’-DDT utilization capacity of the isolate at a lower concentration. While Zn, Cu, Pb, Hg, Ag and Cr showed various patterns of inhibitory effect.

Isolation and Characterization of a Styrene Trimer Degrading Microorganism

2014 ◽  
Vol 1073-1076 ◽  
pp. 666-671
Author(s):  
Guang Chun Li ◽  
Chun Xiang Piao ◽  
Katsuhiko Saido ◽  
Seon Yong Chung
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Liquid Phase ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Ochrobactrum Intermedium ◽  
Complex Strain ◽  
Degrading Microorganism

Biodegradation of the styrene trimer was investigated, and its degrading bacteria were screened and isolated. Complex bacteria ST (strain ST1 and ST2) was isolated from contaminated soil by polystyrene and named by strain ST1 and ST2. ST1 and ST2 were identified by 16S rDNA and classified byOchrobactrum intermediumsp. andPseudomonas aeruginosasp., respectively. Biodegradation experiments were performed in batch and styrene trimer was used as a sole carbon source. Isolated two bacteria were used as degrading microorganisms. Initial liquid phase concentration of the styrene trimer was 50 mg/L. 95% of the styrene trimer was degraded in 17 days by the complex strain ST. The concentration was analyzed by using GC. Metabolites of bacteria were analyzed and three kinds of products that were identified by GC/MS.

Isolation and Characterization of Bacteria That Use Furans as the Sole Carbon Source

2015 ◽  
Vol 178 (1) ◽  
pp. 76-90 ◽  
Author(s):  
Sarah A. Lee ◽  
Lindsey J. Wrona ◽  
A. Bruce Cahoon ◽  
Jacob Crigler ◽  
Mark A. Eiteman ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Isolation And Characterization

Isolation and characterization of a novel thermophilic Bacillus strain degrading long-chain n-alkanes

Extremophiles ◽  
2006 ◽  
Vol 10 (4) ◽  
pp. 347-356 ◽  
Author(s):  
Lei Wang ◽  
Yun Tang ◽  
Shuo Wang ◽  
Ru-Lin Liu ◽  
Mu-Zhi Liu ◽  
...  
Keyword(s):  
Thermophilic Bacillus ◽  
Bacillus Strain ◽  
Isolation And Characterization ◽  
Long Chain

scholarly journals Isolation and Characterization of a Mutant of the Marine Bacterium Alcanivorax borkumensis SK2 Defective in Lipid Biosynthesis

2010 ◽  
Vol 76 (9) ◽  
pp. 2884-2894 ◽  
Author(s):  
Efraín Manilla-Pérez ◽  
Alvin Brian Lange ◽  
Stephan Hetzler ◽  
Marc Wältermann ◽  
Rainer Kalscheuer ◽  
...  
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Marine Bacterium ◽  
Neutral Lipids ◽  
Nile Red ◽  
Dry Weight ◽  
Wax Ester ◽  
Gene Encoding ◽  
Isolation And Characterization ◽  
Key Enzyme

ABSTRACT In many microorganisms, the key enzyme responsible for catalyzing the last step in triacylglycerol (TAG) and wax ester (WE) biosynthesis is an unspecific acyltransferase which is also referred to as wax ester synthase/acyl coenzyme A (acyl-CoA):diacylglycerol acyltransferase (WS/DGAT; AtfA). The importance and function of two AtfA homologues (AtfA1 and AtfA2) in the biosynthesis of TAGs and WEs in the hydrocarbon-degrading marine bacterium Alcanivorax borkumensis SK2 have been described recently. However, after the disruption of both the AtfA1 and AtfA2 genes, reduced but substantial accumulation of TAGs was still observed, indicating the existence of an alternative TAG biosynthesis pathway. In this study, transposon-induced mutagenesis was applied to an atfA1 atfA2 double mutant to screen for A. borkumensis mutants totally defective in biosynthesis of neutral lipids in order to identify additional enzymes involved in the biosynthesis of these lipids. At the same time, we have searched for a totally TAG-negative mutant in order to study the function of TAGs in A. borkumensis. Thirteen fluorescence-negative mutants were identified on Nile red ONR7a agar plates and analyzed for their abilities to synthesize lipids. Among these, mutant 2 M131 was no longer able to synthesize and accumulate TAGs if pyruvate was used as the sole carbon source. The transposon insertion was localized in a gene encoding a putative cytochrome c family protein (ABO_1185). Growth and TAG accumulation experiments showed that the disruption of this gene resulted in the absence of TAGs in 2 M131 but that growth was not affected. In cells of A. borkumensis SK2 grown on pyruvate as the sole carbon source, TAGs represented about 11% of the dry weight of the cells, while in the mutant 2 M131, TAGs were not detected by thin-layer and gas chromatography analyses. Starvation and lipid mobilization experiments revealed that the lipids play an important role in the survival of the cells. The function of neutral lipids in A. borkumensis SK2 is discussed.

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