Molecular characterization of the actin-encoding gene and the use of its promoter for a dominant selection system in the methylotrophic yeast Hansenula polymorpha

2001 ◽  
Vol 55 (6) ◽  
pp. 734-741 ◽  
Author(s):  
H.A. Kang ◽  
W.-K. Hong ◽  
J.-H. Sohn ◽  
E.-S. Choi ◽  
S.K. Rhee
Keyword(s):  
Molecular Characterization ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Selection System ◽  
Encoding Gene

The ClpB protein from Campylobacter jejuni: molecular characterization of the encoding gene and antigenicity of the recombinant protein

Gene ◽  
1999 ◽  
Vol 230 (1) ◽  
pp. 61-67 ◽  
Author(s):  
Frank L Thies ◽  
Helge Karch ◽  
Hans-Peter Hartung ◽  
Gerhard Giegerich
Keyword(s):  
Recombinant Protein ◽  
Molecular Characterization ◽  
Campylobacter Jejuni ◽  
Encoding Gene

Molecular characterization of VP6-encoding gene of group A human rotavirus samples from central west region of Brazil

2008 ◽  
Vol 80 (11) ◽  
pp. 2034-2039 ◽  
Author(s):  
Talissa de Moraes Tavares ◽  
Wilia Marta Elsner Diederichsen de Brito ◽  
Fabíola Souza Fiaccadori ◽  
Juliana Alves Parente ◽  
Paulo Sérgio Sucasas da Costa ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
West Region ◽  
Human Rotavirus ◽  
Group A ◽  
Encoding Gene

scholarly journals The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation.

1995 ◽  
Vol 128 (3) ◽  
pp. 307-319 ◽  
Author(s):  
X Tan ◽  
H R Waterham ◽  
M Veenhuis ◽  
J M Cregg
Keyword(s):  
Amino Acid ◽  
Membrane Protein ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Integral Membrane Protein ◽  
Peroxisome Biogenesis ◽  
Primary Sequence ◽  
Zinc Finger Motifs ◽  
Molecular Machinery

We previously described the isolation of mutants of the methylotrophic yeast Hansenula polymorpha that are defective in peroxisome biogenesis. Here, we describe the characterization of one of these mutants, per8, and the cloning of the PER8 gene. In either methanol or methylamine medium, conditions that normally induce the organelles, per8 cells contain no peroxisome-like structures and peroxisomal enzymes are located in the cytosol. The sequence of PER8 predicts that its product (Per8p) is a novel polypeptide of 34 kD, and antibodies against Per8p recognize a protein of 31 kD. Analysis of the primary sequence of Per8p revealed a 39-amino-acid cysteine-rich segment with similarity to the C3HC4 family of zinc-finger motifs. Overexpression of PER8 results in a markedly enhanced increase in peroxisome numbers. We show that Per8p is an integral membrane protein of the peroxisome and that it is concentrated in the membranes of newly formed organelles. We propose that Per8p is a component of the molecular machinery that controls the proliferation of this organelle.

Cloning and biochemical characterization of hexokinase from the methylotrophic yeast Hansenula polymorpha

2003 ◽  
Vol 44 (5) ◽  
pp. 268-276 ◽  
Author(s):  
Helen Karp ◽  
Aiki J�rviste ◽  
Thomas M. Kriegel ◽  
Tiina Alam�e
Keyword(s):  
Biochemical Characterization ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast

Molecular characterization of the PEX14 gene from the methylotrophic yeast Pichia methanolica

2011 ◽  
Vol 111 (6) ◽  
pp. 624-627 ◽  
Author(s):  
Takashi Ito ◽  
Daisuke Ito ◽  
Shotaro Ozawa ◽  
Shuki Fujimura ◽  
Yoshimi Matsufuji ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Methylotrophic Yeast ◽  
Pichia Methanolica
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