Cloning and biochemical characterization of hexokinase from the methylotrophic yeast Hansenula polymorpha

2003 ◽  
Vol 44 (5) ◽  
pp. 268-276 ◽  
Author(s):  
Helen Karp ◽  
Aiki J�rviste ◽  
Thomas M. Kriegel ◽  
Tiina Alam�e
Keyword(s):  
Biochemical Characterization ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast

scholarly journals The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation.

1995 ◽  
Vol 128 (3) ◽  
pp. 307-319 ◽  
Author(s):  
X Tan ◽  
H R Waterham ◽  
M Veenhuis ◽  
J M Cregg
Keyword(s):  
Amino Acid ◽  
Membrane Protein ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Integral Membrane Protein ◽  
Peroxisome Biogenesis ◽  
Primary Sequence ◽  
Zinc Finger Motifs ◽  
Molecular Machinery

We previously described the isolation of mutants of the methylotrophic yeast Hansenula polymorpha that are defective in peroxisome biogenesis. Here, we describe the characterization of one of these mutants, per8, and the cloning of the PER8 gene. In either methanol or methylamine medium, conditions that normally induce the organelles, per8 cells contain no peroxisome-like structures and peroxisomal enzymes are located in the cytosol. The sequence of PER8 predicts that its product (Per8p) is a novel polypeptide of 34 kD, and antibodies against Per8p recognize a protein of 31 kD. Analysis of the primary sequence of Per8p revealed a 39-amino-acid cysteine-rich segment with similarity to the C3HC4 family of zinc-finger motifs. Overexpression of PER8 results in a markedly enhanced increase in peroxisome numbers. We show that Per8p is an integral membrane protein of the peroxisome and that it is concentrated in the membranes of newly formed organelles. We propose that Per8p is a component of the molecular machinery that controls the proliferation of this organelle.

Cloning and characterization of the gene encoding a repressible acid phosphatase ( PHO1 ) from the methylotrophic yeast Hansenula polymorpha

1998 ◽  
Vol 50 (1) ◽  
pp. 77-84 ◽  
Author(s):  
A. Phongdara ◽  
A. Merckelbach ◽  
P. Keup ◽  
G. Gellissen ◽  
C. P. Hollenberg
Keyword(s):  
Acid Phosphatase ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Gene Encoding

scholarly journals Isolation and characterization of cold sensitive pex6 mutant of the methylotrophic yeast Hansenula polymorpha

2002 ◽  
Vol 18 (2) ◽  
pp. 131-134 ◽  
Author(s):  
V. Y. Nazarko ◽  
O. D. Pochapinsky ◽  
T. Y. Nazarko ◽  
O. V. Stasyk ◽  
A. A. Sibirny
Keyword(s):  
Hansenula Polymorpha ◽  
Methylotrophic Yeast ◽  
Isolation And Characterization ◽  
Cold Sensitive

Isolation and physico-chemical characterization of a cytochrome c from the methylotrophic yeast Hansenula polymorpha

2000 ◽  
Vol 1543 (1) ◽  
pp. 174-188 ◽  
Author(s):  
Marco Borsari ◽  
Elena Dikaya ◽  
Alexander Dikiy ◽  
Mykhailo V Gonchar ◽  
Mykola M Maidan ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Hansenula Polymorpha ◽  
Chemical Characterization ◽  
Methylotrophic Yeast ◽  
Physico Chemical

Purification and characterization of alcohol oxidase from a genetically constructed over-producing strain of the methylotrophic yeast Hansenula polymorpha

2006 ◽  
Vol 71 (3) ◽  
pp. 245-250 ◽  
Author(s):  
S. V. Shleev ◽  
G. P. Shumakovich ◽  
O. V. Nikitina ◽  
O. V. Morozova ◽  
H. M. Pavlishko ◽  
...  
Keyword(s):  
Hansenula Polymorpha ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeast ◽  
Purification And Characterization

Characterization of putative glycosylphosphatidylinositol-anchoring motifs for surface display in the methylotrophic yeast Hansenula polymorpha

2014 ◽  
Vol 36 (10) ◽  
pp. 2085-2094 ◽  
Author(s):  
Seon Ah Cheon ◽  
Jinhee Jung ◽  
Jin Ho Choo ◽  
Doo-Byoung Oh ◽  
Hyun Ah Kang
Keyword(s):  
Hansenula Polymorpha ◽  
Surface Display ◽  
Methylotrophic Yeast
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