Comparison of Cell Number, Viability, Phenotypic Profile, Clonogenic, and Proliferative Potential of Adipose-Derived Stem Cell Populations Between Centrifuged and Noncentrifuged Fat

2014 ◽  
Vol 38 (5) ◽  
pp. 985-993 ◽  
Author(s):  
Adalberto Ibatici ◽  
Fabio Caviggioli ◽  
Vinci Valeriano ◽  
Nadia Quirici ◽  
Nadia Sessarego ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Number ◽  
Cell Populations ◽  
Proliferative Potential ◽  
Phenotypic Profile ◽  
Stem Cell Populations ◽  
Adipose Derived Stem Cell

scholarly journals Quiescent, Slow-Cycling Stem Cell Populations in Cancer: A Review of the Evidence and Discussion of Significance

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Nathan Moore ◽  
Stephen Lyle
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Adult Stem Cells ◽  
Cell Populations ◽  
Proliferative Potential ◽  
Cell Cycle Regulators ◽  
Slow Cycling ◽  
Stem Cell Populations

Long-lived cancer stem cells (CSCs) with indefinite proliferative potential have been identified in multiple epithelial cancer types. These cells are likely derived from transformed adult stem cells and are thought to share many characteristics with their parental population, including a quiescent slow-cycling phenotype. Various label-retaining techniques have been used to identify normal slow cycling adult stem cell populations and offer a unique methodology to functionally identify and isolate cancer stem cells. The quiescent nature of CSCs represents an inherent mechanism that at least partially explains chemotherapy resistance and recurrence in posttherapy cancer patients. Isolating and understanding the cell cycle regulatory mechanisms of quiescent cancer cells will be a key component to creation of future therapies that better target CSCs and totally eradicate tumors. Here we review the evidence for quiescent CSC populations and explore potential cell cycle regulators that may serve as future targets for elimination of these cells.

scholarly journals The Adipose-derived Stem Cell: Looking Back and Looking Ahead

2010 ◽  
Vol 21 (11) ◽  
pp. 1783-1787 ◽  
Author(s):  
Patricia A. Zuk
Keyword(s):  
Stem Cell ◽  
Historical Perspective ◽  
Es Cells ◽  
Adult Stem Cell ◽  
Stem Cell Population ◽  
Cell Populations ◽  
Stem Cell Populations ◽  
New Applications ◽  
Adipose Derived Stem Cell ◽  
Looking Back

In 2002, researchers at UCLA published a manuscript in Molecular Biology of the Cell describing a novel adult stem cell population isolated from adipose tissue—the adipose-derived stem cell (ASC). Since that time, the ASC has gone on to be one of the most popular adult stem cell populations currently being used in the stem cell field. With multilineage mesodermal potential and possible ectodermal and endodermal potentials also, the ASC could conceivably be an alternate to pluripotent ES cells in both the lab and in the clinic. In this retrospective article, a historical perspective on the ASC is given together with exciting new applications for the stem cell being considered today.

scholarly journals Recombinant rat stem cell factor stimulates the amplification and differentiation of fractionated mouse stem cell populations

Blood ◽  
1992 ◽  
Vol 79 (1) ◽  
pp. 58-64 ◽  
Author(s):  
N Williams ◽  
I Bertoncello ◽  
H Kavnoudias ◽  
K Zsebo ◽  
I McNiece
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Stem Cell Factor ◽  
Single Cells ◽  
Marrow Cell ◽  
Cell Number ◽  
Cell Populations ◽  
Proliferative Potential ◽  
Agar Culture ◽  
Gm Csf

Abstract The role of recombinant rat stem cell factor (rrSCF) was studied on defined primitive bone marrow cell populations. In agar culture of 500 lineage-negative/Sca-1-positive (Lin-/Sca-1+) cells, rrSCF alone stimulates small colonies of predominantly granulocytic cells. The combinations of rrSCF plus interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), or macrophage CSF (CSF-1) stimulated primitive progenitor cells defined as high proliferative potential colony-forming cells (HPP-CFC). Synergistic increases in total colony numbers were obtained with rrSCF plus GM-CSF, granulocyte CSF (G-CSF), CSF-1, or IL-6, but not IL-1 or IL-3. Lin-/Sca-1+ cells were incubated in liquid culture at 3,000 cells/mL for 6 days in the presence of rrSCF alone or in combination with other growth factors. The total number of cells was increased twofold in the presence of rrSCF, with the progeny primarily myeloid in nature. The greatest increase in cell number was obtained with rrSCF plus IL-3, where the cell number increased 40-fold. These factors also stimulated an increase in HPP-CFC (10-fold) and GM-CFC (500-fold). To determine if these interactions were direct, single Lin-/Sca-1+ cells were sorted into microtiter wells and the cell proliferation scored 6 days later. RrSCF synergized with IL-3, IL-6, and G-CSF to stimulate the proliferation of single cells. The cells in positive wells were subcultured into colony-forming assays and up to 400 CFC per well were obtained after 14 days incubation of the secondary cultures. These data demonstrate that rrSCF acts in combination with various growth factors to directly stimulate the amplification potential of hematopoietic primitive precursors, resulting in differentiation of these precursors.

scholarly journals Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations

2012 ◽  
Vol 13 (4) ◽  
pp. 413 ◽  
Author(s):  
Jae-Jun Bahn ◽  
Jin-Young Chung ◽  
Wooseok Im ◽  
Manho Kim ◽  
Sae Hoon Kim
Keyword(s):  
Stem Cell ◽  
Autologous Serum ◽  
Cell Populations ◽  
Stem Cell Populations ◽  
Adipose Derived Stem Cell

scholarly journals Recombinant rat stem cell factor stimulates the amplification and differentiation of fractionated mouse stem cell populations

Blood ◽  
1992 ◽  
Vol 79 (1) ◽  
pp. 58-64
Author(s):  
N Williams ◽  
I Bertoncello ◽  
H Kavnoudias ◽  
K Zsebo ◽  
I McNiece
Keyword(s):  
Stem Cell ◽  
Growth Factors ◽  
Stem Cell Factor ◽  
Single Cells ◽  
Marrow Cell ◽  
Cell Number ◽  
Cell Populations ◽  
Proliferative Potential ◽  
Agar Culture ◽  
Gm Csf

The role of recombinant rat stem cell factor (rrSCF) was studied on defined primitive bone marrow cell populations. In agar culture of 500 lineage-negative/Sca-1-positive (Lin-/Sca-1+) cells, rrSCF alone stimulates small colonies of predominantly granulocytic cells. The combinations of rrSCF plus interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), or macrophage CSF (CSF-1) stimulated primitive progenitor cells defined as high proliferative potential colony-forming cells (HPP-CFC). Synergistic increases in total colony numbers were obtained with rrSCF plus GM-CSF, granulocyte CSF (G-CSF), CSF-1, or IL-6, but not IL-1 or IL-3. Lin-/Sca-1+ cells were incubated in liquid culture at 3,000 cells/mL for 6 days in the presence of rrSCF alone or in combination with other growth factors. The total number of cells was increased twofold in the presence of rrSCF, with the progeny primarily myeloid in nature. The greatest increase in cell number was obtained with rrSCF plus IL-3, where the cell number increased 40-fold. These factors also stimulated an increase in HPP-CFC (10-fold) and GM-CFC (500-fold). To determine if these interactions were direct, single Lin-/Sca-1+ cells were sorted into microtiter wells and the cell proliferation scored 6 days later. RrSCF synergized with IL-3, IL-6, and G-CSF to stimulate the proliferation of single cells. The cells in positive wells were subcultured into colony-forming assays and up to 400 CFC per well were obtained after 14 days incubation of the secondary cultures. These data demonstrate that rrSCF acts in combination with various growth factors to directly stimulate the amplification potential of hematopoietic primitive precursors, resulting in differentiation of these precursors.

scholarly journals Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag

2021 ◽  
Vol 2 (3) ◽  
pp. 100751
Author(s):  
Yuefeng Li ◽  
Kiran Nakka ◽  
Thomas Olender ◽  
Philippe Gingras-Gelinas ◽  
Matthew Man-Kin Wong ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Stem Cell ◽  
Cell Populations ◽  
Stem Cell Populations

Notch Signaling Is Required to Maintain All Neural Stem Cell Populations – Irrespective of Spatial or Temporal Niche

2006 ◽  
Vol 28 (1-2) ◽  
pp. 34-48 ◽  
Author(s):  
Tania O. Alexson ◽  
Seiji Hitoshi ◽  
Brenda L. Coles ◽  
Alan Bernstein ◽  
Derek van der Kooy
Keyword(s):  
Stem Cell ◽  
Notch Signaling ◽  
Neural Stem Cell ◽  
Cell Populations ◽  
Temporal Niche ◽  
Stem Cell Populations

scholarly journals Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0140143 ◽  
Author(s):  
Maria Fernanda Forni ◽  
Aline Ramos Maia Lobba ◽  
Alexandre Hamilton Pereira Ferreira ◽  
Mari Cleide Sogayar
Keyword(s):  
Stem Cell ◽  
Cell Populations ◽  
Stem Cell Populations

scholarly journals Activation of two distinct Sox9-EGFP-expressing intestinal stem cell populations during crypt regeneration after irradiation

2012 ◽  
Vol 302 (10) ◽  
pp. G1111-G1132 ◽  
Author(s):  
Laurianne Van Landeghem ◽  
M. Agostina Santoro ◽  
Adrienne E. Krebs ◽  
Amanda T. Mah ◽  
Jeffrey J. Dehmer ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Populations ◽  
Intestinal Epithelial ◽  
P53 Signaling ◽  
Reporter Mice ◽  
Radiation Induced ◽  
Molecular Phenotypes ◽  
Stem Cell Populations ◽  
Induced Changes

Recent identification of intestinal epithelial stem cell (ISC) markers and development of ISC reporter mice permit visualization and isolation of regenerating ISCs after radiation to define their functional and molecular phenotypes. Previous studies in uninjured intestine of Sox9-EGFP reporter mice demonstrate that ISCs express low levels of Sox9-EGFP (Sox9-EGFP Low), whereas enteroendocrine cells (EEC) express high levels of Sox9-EGFP (Sox9-EGFP High). We hypothesized that Sox9-EGFP Low ISCs would expand after radiation, exhibit enhanced proliferative capacities, and adopt a distinct gene expression profile associated with rapid proliferation. Sox9-EGFP mice were given 14 Gy abdominal radiation and studied between days 3 and 9 postradiation. Radiation-induced changes in number, growth, and transcriptome of the different Sox9-EGFP cell populations were determined by histology, flow cytometry, in vitro culture assays, and microarray. Microarray confirmed that nonirradiated Sox9-EGFP Low cells are enriched for Lgr5 mRNA and mRNAs enriched in Lgr5-ISCs and identified additional putative ISC markers. Sox9-EGFP High cells were enriched for EEC markers, as well as Bmi1 and Hopx, which are putative markers of quiescent ISCs. Irradiation caused complete crypt loss, followed by expansion and hyperproliferation of Sox9-EGFP Low cells. From nonirradiated intestine, only Sox9-EGFP Low cells exhibited ISC characteristics of forming organoids in culture, whereas during regeneration both Sox9-EGFP Low and High cells formed organoids. Microarray demonstrated that regenerating Sox9-EGFP High cells exhibited transcriptomic changes linked to p53-signaling and ISC-like functions including DNA repair and reduced oxidative metabolism. These findings support a model in which Sox9-EGFP Low cells represent active ISCs, Sox9-EGFP High cells contain radiation-activatable cells with ISC characteristics, and both participate in crypt regeneration.

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