The Effects of Retinoic Acid on the Insulin-like Growth Factor Axis in Primary Tissue Culture from Hyperparathyroidism

2006 ◽  
Vol 30 (5) ◽  
pp. 714-720 ◽  
Author(s):  
Chris K.M. Wong ◽  
Teresa Lai ◽  
Jeffrey M.P. Holly ◽  
Malcolm H. Wheeler ◽  
Claire E.H. Stewart ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Retinoic Acid ◽  
Growth Factor ◽  
Insulin Like Growth Factor ◽  
Primary Tissue Culture ◽  
Primary Tissue

Retinoic acid regulates insulin-like growth factor II expression in a neuroblastoma cell line.

Endocrinology ◽  
1992 ◽  
Vol 130 (6) ◽  
pp. 3669-3676 ◽  
Author(s):  
K Matsumoto ◽  
C Gaetano ◽  
W H Daughaday ◽  
C J Thiele
Keyword(s):  
Retinoic Acid ◽  
Growth Factor ◽  
Cell Line ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Insulin Like Growth Factor ◽  
Factor Ii

scholarly journals Down-regulation of insulin-like growth factor binding proteins and growth modulation in hepatoma cells by retinoic acid

Hepatology ◽  
1999 ◽  
Vol 29 (4) ◽  
pp. 1091-1098 ◽  
Author(s):  
Dae-Ghon Kim ◽  
Dae-yeol Lee ◽  
Baik-Hwan Cho ◽  
Kyung-Ran You ◽  
Mi-Young Kim ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Growth Factor ◽  
Binding Proteins ◽  
Hepatoma Cells ◽  
Insulin Like Growth Factor ◽  
Growth Modulation ◽  
Down Regulation ◽  
Factor Binding

Effects of ACTH and 3?,5?-cyclic purine nucleotides on the morphology and metabolism of normal adult human adrenocortical cells in primary tissue culture

1978 ◽  
Vol 190 (2) ◽  
Author(s):  
Ubaldo Armato ◽  
GastoneG. Nussdorfer ◽  
Giuliano Neri ◽  
Enrica Draghi ◽  
PaolaG. Andreis ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Normal Adult ◽  
Purine Nucleotides ◽  
Adrenocortical Cells ◽  
Adult Human ◽  
Primary Tissue Culture ◽  
Primary Tissue

Modulation by retinoic acid of insulin-like growth factor (IGF) and IGF binding protein expression in human SK-N-SH neuroblastoma cells

1996 ◽  
Vol 134 (4) ◽  
pp. 474-480 ◽  
Author(s):  
Sylvie Babajko ◽  
Michel Binoux
Keyword(s):  
Retinoic Acid ◽  
Growth Factor ◽  
Protein Expression ◽  
Binding Protein ◽  
Neuroblastoma Cells ◽  
Insulin Like Growth Factor ◽  
Igf Binding ◽  
Igf Receptor ◽  
Igf Binding Protein

Babajko S, Binoux M. Modulation by retinoic acid of insulin-like growth factor (IGF) and IGF binding protein expression in human SK-N-SH neuroblastoma cells. Eur J Endocrinol 1996;134:474–80. ISSN 0804–4643 Growth in neuroblastoma cells is regulated by insulin-like growth factors (IGFs) whose action is modulated by IGF binding proteins (IGFBPs). In this study, SK-N-SH neuroblastoma cells were shown to produce IGF-II, IGFBP-2, IGFBP-4 and small quantities of IGFBP-6. We have studied the effects of a natural morphogen, retinoic acid (RA), on growth and IGFBP expression in these cells. In all experiments, cells were cultured in serum-free medium and treated with 1 μmol/l RA for 12 h. Cell number increased by almost 50% during the first 24 h after the beginning of treatment. This stimulation was inhibited by 80% or more in the presence of the anti-type 1 IGF receptor antibody α-IR3 and anti-IGF-II antibody. The IGF-II concentrations in the culture media, measured after acidic gel filtration, increased about 1.5-fold and Northern blotting showed a concomitant increase in IGF-II mRNA levels. The mitogenic effect of RA therefore reflects its stimulation of IGF-II production. The availability of IGF-II to the cells may also be enhanced because of the proteolysis of IGFBP-2 to which it is bound. After this initial phase, proliferation ceased despite continued IGF-II production between 24 and 72 h. Both IGFBP-2 and IGFBP-4 production decreased, whereas that of IGFBP-6 increased. These changes appeared both in the protein quantities and in their mRNAs. Insulin-like growth factor binding protein 6 has a strong affinity for IGF-II, 5–10 times that of IGFBP-2 and at least 10 times that of the type 1 IGF receptor, and the arrested proliferation may result, at least in part, from sequestration by IGFBP-6 of the IGF-II secreted. Sylvie Babajko, INSERM U142, Hôpital Saint Antoine, 184 rue du Faubourg, St Antoine, 75571 Paris Cedex 12, France

scholarly journals Growth and contractility of cells from fibrocellular epiretinal membranes in primary tissue culture.

1988 ◽  
Vol 72 (2) ◽  
pp. 116-126 ◽  
Author(s):  
D. Y. Jiang ◽  
P. S. Hiscott ◽  
I. Grierson ◽  
D. McLeod
Keyword(s):  
Tissue Culture ◽  
Epiretinal Membranes ◽  
Primary Tissue Culture ◽  
Primary Tissue
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