A Short Region from the LEU2 Gene of Saccharomyces cerevisiae Functions as an ARS in the Yeast Saccharomyces exiguus Yp74L-3

1998 ◽  
Vol 37 (6) ◽  
pp. 426-430 ◽  
Author(s):  
Taisuke Hisatomi ◽  
Yutaka Wada ◽  
Chiaki Fujisaki ◽  
Michio Tsuboi
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Short Region ◽  
Leu2 Gene ◽  
Saccharomyces Exiguus

scholarly journals Evaluación de levaduras en la producción de etanol a partir de melaza de caña de azúcar

2020 ◽  
Vol 11 (2) ◽  
pp. 115-119
Author(s):  
Miguel Alejandro Tuárez Párraga ◽  
◽  
Mabel Leonela Laz Mero ◽  
Stephany Judith Bermello Ochoa ◽  
◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Utilis ◽  
Microsoft Excel ◽  
C 30 ◽  
Saccharomyces Exiguus

El objetivo de la investigación consistió en evaluar el efecto de la adición de tres tipos de levaduras comerciales Saccharomyces cerevisiae, Saccharomyces exiguus y Candida utilis, utilizando disolución de melaza, las etapas del experimento consistieron en la activación de levadura y fermentación para la producción de etanol mediante una destilación simple; determinando la producción de etanol más óptima del experimento, con diferentes concentraciones de sacarosa (19°Bx, 23°Bx y 27°Bx) y diferentes condiciones ambientales (27°C, 30°C y 33°C). Se aplicó un modelo estadístico de diseño cuadrado latino utilizando el programa Microsoft Excel, tomando como factor de interés tipos de levaduras y factores secundarios los sólidos solubles (°Bx) y temperaturas de almacenamiento (°C). Se formularon nueve tratamientos (T), de los cuales se observaron diferencias significativas (p<0,05) entre los sólidos solubles (°Bx) y (p>0,05) para los tipos de levaduras estudiadas y las temperaturas de almacenamiento. Durante la producción de etanol a partir de la disolución de melaza de caña, el mejor tratamiento fue el T8, utilizando levadura Saccharomyces cerevisiae a 27°Bx y con temperatura de 30°C.

Nucleotide sequence of the 3' terminal region of the LEU2 gene from Saccharomyces cerevisiae

Gene ◽  
1984 ◽  
Vol 31 (1-3) ◽  
pp. 257-261 ◽  
Author(s):  
Byron E. Froman ◽  
Robert C. Tait ◽  
Raymond L. Rodriguez
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Terminal Region ◽  
Leu2 Gene

scholarly journals Genetic mapping of Ty elements in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (2) ◽  
pp. 329-339 ◽  
Author(s):  
H L Klein ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Statistical Analysis ◽  
Genetic Mapping ◽  
Transposable Element ◽  
Genetic Map ◽  
Selectable Marker ◽  
Repeated Element ◽  
Ty Elements ◽  
Reciprocal Exchange ◽  
Leu2 Gene

We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a reciprocal exchange using homology at the LEU2 locus or at the various Ty elements that are dispersed throughout the S. cerevisiae genome. These transformants were used to map genetically sites of several Ty elements. The 24 transformants recovered at Ty sites define 19 distinct loci. Seven of these were placed on the genetic map. Two classes of Ty elements were identified in these experiments: a Ty1-17 class and Ty elements different from Ty1-17. Statistical analysis of the number of transformants at each class of Ty elements shows that there is preferential integration of the CV9 plasmid into the Ty1-17 class.

Extramitochondrial citrate synthase activity in bakers' yeast

1986 ◽  
Vol 6 (2) ◽  
pp. 488-493
Author(s):  
T M Rickey ◽  
A S Lewin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Citrate Synthase ◽  
Glucose Repression ◽  
Yeast Cells ◽  
Mitochondrial Enzyme ◽  
Wild Type ◽  
Reading Frame ◽  
Leu2 Gene ◽  
Growth Requirements ◽  
Wild Type Yeast

We isolated the gene for citrate synthase (citrate oxaloacetate lyase; EC 4.1.3.7) from Saccharomyces cerevisiae and ablated it by inserting the yeast LEU2 gene within its reading frame. This revealed a second, nonmitochondrial citrate synthase. Like the mitochondrial enzyme, this enzyme was sensitive to glucose repression. It did not react with antibodies against mitochondrial citrate synthase. Haploid cells lacking a gene for mitochondrial citrate synthase grew somewhat slower than wild-type yeast cells, but exhibited no auxotrophic growth requirements.

Cloning of the LEU2 gene of Saccharomyces cerevisiae by in vivo recombination

1989 ◽  
Vol 152 (3) ◽  
pp. 263-268 ◽  
Author(s):  
R. Valinger ◽  
G. Braus ◽  
P. Niederberger ◽  
M. K�nzler ◽  
G. Paravicini ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Leu2 Gene ◽  
In Vivo Recombination

scholarly journals RECOMBINATION BETWEEN GENES LOCATED ON NONHOMOLOGOUS CHROMOSOMES IN SACCHAROMYCES CEREVISIAE

Genetics ◽  
1982 ◽  
Vol 101 (3-4) ◽  
pp. 369-404
Author(s):  
Margaret Dubay Mikus ◽  
Thomas D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Interaction ◽  
Common Type ◽  
Genetic Interactions ◽  
The Other ◽  
Physical Analysis ◽  
Ura3 Gene ◽  
Leu2 Gene ◽  
Transfer Of Information ◽  
Repeated Genes

ABSTRACT We constructed strains of Saccharomyces cerevisiae that contained two different mutant alleles of either the leu2 gene or the ura3 gene. These repeated genes were located on nonhomologous chromosomes; the two ura3- alleles were located on chromosomes V and XII and the two leu2- alleles were located on chromosomes III and XII. Genetic interactions between the two mutant copies of a gene were detected by the generation of either Leu+ or Ura+ revertants. Both spontaneous and ultraviolet irradiation-induced revertants were examined. By genetic and physical analysis, we have shown that Leu+ or Ura+ revertants can arise by a variety of different genetic interactions. The most common type of genetic interaction is the nonreciprocal transfer of information from one repeat to the other. We also detected reciprocal recombination between repeated genes, resulting in reciprocally translocated chromosomes.

Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae

1987 ◽  
Vol 7 (6) ◽  
pp. 2087-2096
Author(s):  
B Sauer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Functional Expression ◽  
Yeast Saccharomyces Cerevisiae ◽  
Efficient Manner ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Leu2 Gene ◽  
Recombination System ◽  
A Site ◽  
Site Specific Recombination System

The procaryotic cre-lox site-specific recombination system of coliphage P1 was shown to function in an efficient manner in a eucaryote, the yeast Saccharomyces cerevisiae. The cre gene, which codes for a site-specific recombinase, was placed under control of the yeast GALI promoter. lox sites flanking the LEU2 gene were integrated into two different chromosomes in both orientations. Excisive recombination at the lox sites (as measured by loss of the LEU2 gene) was promoted efficiently and accurately by the Cre protein and was dependent upon induction by galactose. These results demonstrate that a procaryotic recombinase can enter a eucaryotic nucleus and, moreover, that the ability of the Cre recombinase to perform precise recombination events on the chromosomes of S. cerevisiae is unimpaired by chromatin structure.

In vivo construction of linear vectors based on killer plasmids from Kluyveromyces lactis: selection of a nuclear gene results in attachment of telomeres

1989 ◽  
Vol 9 (9) ◽  
pp. 3931-3937
Author(s):  
J Kämper ◽  
F Meinhardt ◽  
N Gunge ◽  
K Esser
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Kluyveromyces Lactis ◽  
Nuclear Gene ◽  
Leu2 Gene ◽  
In Vivo Recombination ◽  
Hybrid Vectors ◽  
Hybrid Molecules ◽  
Specific Sequences ◽  
Selection Of

Linear vectors based on plasmids pGKL1 and pGKL2 from Kluyveromyces lactis were obtained by in vivo recombination in Saccharomyces cerevisiae and selected for integration of the nuclear LEU2 gene. The linear hybrid molecules obtained had no proteins attached to their 5' ends, as is found for native pGKL plasmids. However, telomere-specific sequences were added to the ends of pGKL1. In contrast to the cytoplasmically localized pGKL plasmids, the newly obtained linear hybrid vectors probably replicate within the nucleus and provide evidence that the nuclear LEU2 gene cannot be expressed in the cytoplasm.

Fusion of the Saccharomyces cerevisiae leu2 gene to an Escherichia coli beta-galactosidase gene

1983 ◽  
Vol 3 (4) ◽  
pp. 580-586
Author(s):  
A E Martinez-Arias ◽  
M J Casadaban
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Translation Initiation ◽  
Agar Medium ◽  
Yeast Cells ◽  
Galactosidase Activity ◽  
Chromogenic Substrates ◽  
Translation Initiation Region ◽  
Leu2 Gene ◽  
Beta Galactosidase

The promoter and translation initiation region of the Saccharomyces cerevisiae leu2 gene was fused to the Escherichia coli beta-galactosidase gene. This fusion located the control region of the leu gene and orientated its direction of expression. When the fusion was placed into yeast cells, beta-galactosidase was expressed under the same regulatory pattern as the original leu2 gene product: its synthesis was repressed in the presence of leucine and threonine. Sensitive chromogenic substrates for beta-galactosidase were used to detect expression in isolated colonies growing on agar medium. Mutant yeast cells with increased beta-galactosidase activity were identified by the color of the colonies they formed. One class of mutants obtained appeared to affect ars1 plasmid maintenance, and another class appeared to affect beta-galactoside uptake.

Genetic mapping of Ty elements in Saccharomyces cerevisiae

1984 ◽  
Vol 4 (2) ◽  
pp. 329-339
Author(s):  
H L Klein ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Statistical Analysis ◽  
Genetic Mapping ◽  
Transposable Element ◽  
Genetic Map ◽  
Selectable Marker ◽  
Repeated Element ◽  
Ty Elements ◽  
Reciprocal Exchange ◽  
Leu2 Gene

We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a reciprocal exchange using homology at the LEU2 locus or at the various Ty elements that are dispersed throughout the S. cerevisiae genome. These transformants were used to map genetically sites of several Ty elements. The 24 transformants recovered at Ty sites define 19 distinct loci. Seven of these were placed on the genetic map. Two classes of Ty elements were identified in these experiments: a Ty1-17 class and Ty elements different from Ty1-17. Statistical analysis of the number of transformants at each class of Ty elements shows that there is preferential integration of the CV9 plasmid into the Ty1-17 class.

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