Das molekularpathologische Brevier: was heißt: „hybrid capture“,„anchored multiplex PCR“ und amplikonbasiert?

Study Comparing Human Papillomavirus (HPV) Real-Time Multiplex PCR and Hybrid Capture II INNO-LiPA v2 HPV Genotyping PCR Assays

Journal of Clinical Microbiology ◽

10.1128/jcm.01907-08 ◽

2009 ◽

Vol 47 (7) ◽

pp. 2106-2113 ◽

Cited By ~ 19

Author(s):

T. Iftner ◽

L. Germ ◽

R. Swoyer ◽

S. K. Kjaer ◽

J. G. Breugelmans ◽

...

Keyword(s):

Human Papillomavirus ◽

Multiplex Pcr ◽

Real Time ◽

Hybrid Capture ◽

Hpv Genotyping ◽

Pcr Assays

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A multiplex PCR-SSCP test to genotype bovine β-casein alleles A1 , A2 , A3 , B, and C

Animal Genetics ◽

10.1046/j.1365-2052.1999.00445-6.x ◽

1999 ◽

Vol 30 (4) ◽

pp. 322-323 ◽

Cited By ~ 9

Author(s):

A. Barroso ◽

S. Dunner ◽

J. Ca·ón

Keyword(s):

Multiplex Pcr

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Multiplex PCR zum Nachweis disseminierter Tumorzellen (DTC) im Blut von Patientinnen mit Mammakarzinom

Zentralblatt für Gynäkologie ◽

10.1055/s-2005-870717 ◽

2005 ◽

Vol 127 (03) ◽

Author(s):

J Rom ◽

A Schneeeweiss ◽

V Zieglschmid ◽

C Hollmann ◽

O Böcher ◽

...

Keyword(s):

Multiplex Pcr

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Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v1i2.21506 ◽

2016 ◽

Vol 1 (2) ◽

pp. 38-42 ◽

Cited By ~ 4

Author(s):

Khairun Nessa ◽

Dilruba Ahmed ◽

Johirul Islam ◽

FM Lutful Kabir ◽

M Anowar Hossain

Keyword(s):

Escherichia Coli ◽

Multiplex Pcr ◽

Clinical Laboratory ◽

Proteinase K ◽

Microbiology Laboratory ◽

Pcr Assay ◽

E Coli ◽

Diarrheagenic Escherichia Coli ◽

Multiplex Pcr Assay ◽

Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

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