The Bax inhibitor UvBI-1, a negative regulator of mycelial growth and conidiation, mediates stress response and is critical for pathogenicity of the rice false smut fungus Ustilaginoidea virens

2019 ◽  
Vol 65 (5) ◽  
pp. 1185-1197 ◽  
Author(s):  
Songlin Xie ◽  
Yufu Wang ◽  
Wei Wei ◽  
Chongyang Li ◽  
Yi Liu ◽  
...  
Keyword(s):  
Stress Response ◽  
Mycelial Growth ◽  
Negative Regulator ◽  
Smut Fungus ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
False Smut

Two protein kinases UvPmk1 and UvCDC2 with significant functions in conidiation, stress response and pathogenicity of rice false smut fungus Ustilaginoidea virens

2019 ◽  
Vol 66 (2) ◽  
pp. 409-420 ◽  
Author(s):  
Jintian Tang ◽  
Jing Bai ◽  
Xiaoyang Chen ◽  
Lu Zheng ◽  
Hao Liu ◽  
...  
Keyword(s):  
Stress Response ◽  
Protein Kinases ◽  
Smut Fungus ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
False Smut

scholarly journals Transcription factor UvMsn2 is important for vegetative growth, conidiogenesis, stress response, mitochondrial morphology and pathogenicity in the rice false smut fungus Ustilaginoidea virens

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Yuandi Xu ◽  
Shuang Wu ◽  
Zhaomeng Yu ◽  
Emily Kolojane Moeketsi ◽  
Zhixiang Yang ◽  
...  
Keyword(s):  
Stress Response ◽  
Vegetative Growth ◽  
Rice Blast Fungus ◽  
Smut Fungus ◽  
Mitochondrial Morphology ◽  
Rice Seed ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
Aerial Hyphae ◽  
False Smut

AbstractTranscription factors (TFs) play critical roles in the control of development and pathogenicity of phytopathogens by directly or indirectly regulating the expression of downstream genes. Here, we identified and characterized a zinc finger TF UvMsn2 in Ustilaginoidea virens, a homolog of MoMsn2 from the rice blast fungus. Heterogenous complementation assay revealed that UvMsn2 fully restored the defects of the ∆Momsn2 mutant in vegetative growth, conidiation and pathogenicity. Deletion of UvMsn2 in U. virens led to a reduction of the pathogen in vegetative growth, aerial hyphae and conidiation. Additionally, the ∆Uvmsn2 mutant displayed defects in conidial morphology and germination, as well as mitochondrial morphology. Pathogenicity and toxicity assays revealed that the ∆Uvmsn2 mutant was non-pathogenic and less inhibitory to rice seed germination. The ∆Uvmsn2 mutant showed different sensitivity to various stresses. Further microscopic examination found that UvMsn2 was localized in both cytosol and nucleus, and translocated from cytosol to nucleus under the treatment of NaCl. Our results demonstrate that UvMsn2 is a critical TF that regulates the vegetative growth, conidiogenesis, stress response, mitochondrial morphology and virulence in the rice false smut fungus.

scholarly journals A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1563-1579
Author(s):  
Xiaoyang Chen ◽  
Pingping Li ◽  
Hao Liu ◽  
Xiaolin Chen ◽  
Junbin Huang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Smut Fungus ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
False Smut

scholarly journals UvHOG1 is important for hyphal growth and stress responses in the rice false smut fungus Ustilaginoidea virens

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Dawei Zheng ◽  
Yi Wang ◽  
Yu Han ◽  
Jin-Rong Xu ◽  
Chenfang Wang
Keyword(s):  
Stress Responses ◽  
Hyphal Growth ◽  
Smut Fungus ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
False Smut

scholarly journals UvSMEK1, a Suppressor of MEK Null, Regulates Pathogenicity, Conidiation and Conidial Germination in Rice False Smut Fungus Ustilaginoidea virens

Rice Science ◽  
2021 ◽  
Vol 28 (5) ◽  
pp. 457-465
Author(s):  
Yu Junjie ◽  
Yu Mina ◽  
Song Tianqiao ◽  
Cao Huijuan ◽  
Yong Mingli ◽  
...  
Keyword(s):  
Conidial Germination ◽  
Smut Fungus ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
False Smut

The nucleotide sequence and genome organization of two victoriviruses from the rice false smut fungus Ustilaginoidea virens

Virus Genes ◽  
2014 ◽  
Vol 48 (3) ◽  
pp. 570-573 ◽  
Author(s):  
Jie Zhong ◽  
Qian Zhou ◽  
Xiang Hua Lei ◽  
Dan Chen ◽  
Hong Hong Shang ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Genome Organization ◽  
Smut Fungus ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
False Smut

scholarly journals The cyclase-associated protein UvCap1 is required for mycelial growth and pathogenicity in the rice false smut fungus

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Hui-Juan Cao ◽  
Jin-Jin Zhang ◽  
Ming-Li Yong ◽  
Mi-Na Yu ◽  
Tian-Qiao Song ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Mycelial Growth ◽  
Pathogenic Fungi ◽  
Camp Signaling ◽  
Deletion Mutants ◽  
Intracellular Camp ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
Camp Signaling Pathway ◽  
False Smut

AbstractRice false smut caused byUstilaginoidea virensis one of the widespread rice diseases across the globe in recent years, however, we know little about its molecular mechanism of infection. The cAMP signaling pathway functions directly in the development and formation of infectious structures to regulate the infection process in many pathogenic fungi. In order to investigate the role of the cAMP signaling pathway inU. virens, UvCap1, a cyclase-associated-protein homologous toSaccharomyces cerevisiaeSrv2 was identified. Three targeted deletion mutants of theUvCAP1gene were obtained with gene replacement strategy assisted with CRISPR-Cas9 system. TheUvCAP1deletion mutants showed defects in mycelial growth and conidial production. Inoculation experiments demonstrated that ΔUvcap1exhibited defects in pathogenicity. Compared with the wild-type strain, ΔUvcap1showed decreased tolerance to sorbitol and H2O2, and increased tolerance to NaCl, CFW and SDS, and the intracellular cAMP level was significantly reduced in ΔUvcap1. Yeast two-hybrid assay identified the interactions of UvCap1 with UvAc1 (adenylase cyclase), two Ras proteins (UvRas1 and UvRas2) and UvSte50. Taken together, as a component of cAMP signaling pathway, UvCap1 plays important roles in the development and pathogenicity ofU. virens.

scholarly journals Identification and characterization of the Nep1-like protein UvNLP in rice false smut fungus Ustilaginoidea virens

2020 ◽  
Author(s):  
Yufu Wang ◽  
Jinsong Qu ◽  
Yueran Liu ◽  
Geng Sun ◽  
Zuoqian Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Real Time ◽  
Real Time Pcr ◽  
Plant Defence ◽  
Smut Fungus ◽  
Post Inoculation ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
False Smut ◽  
Defence Responses

Abstract Background The necrosis- and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) are a broadly distributed superfamily of protiens in plant-associated microorganisms, including bacteria, fungi, and oomycetes. NLPs are considered to be important virulence factors, and some have been well studied. However, the role of NLPs in Ustilaginoidea virens , the agent of rice false smut fungus, remains to be uncovered.Results A protein containing NLP-specific NPP1 domain was identified in U. virens and named UvNLP. Phylogenetic analysis revealed that UvNLP was a type 2 NLP, and real-time PCR revealed that UvNLP was highly expressed at 3 days post-inoculation. A yeast secretion assay demonstrated that UvNLP contained a functional signal peptide (SP). Furthermore, transient expression of UvNLP in Nicotiana benthamiana induced weak cell death, and replacement of the SP enhanced cell death. Real-time PCR indicated that UvNLP induced the expression of plant defence related genes.Conclusions We identified a secreted protein UvNLP in U. virens that was infection related and could induce plant cell death and defence responses. This study provided evidence that NLPs act as proteinaceous MAMPs, giving insight into the elucidation of the pathogenicity mechanism of U. virens. The UvNLP might be used as a potential plant defence inducer for disease control in practice.

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