scholarly journals The cyclase-associated protein UvCap1 is required for mycelial growth and pathogenicity in the rice false smut fungus

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Hui-Juan Cao ◽  
Jin-Jin Zhang ◽  
Ming-Li Yong ◽  
Mi-Na Yu ◽  
Tian-Qiao Song ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Mycelial Growth ◽  
Pathogenic Fungi ◽  
Camp Signaling ◽  
Deletion Mutants ◽  
Intracellular Camp ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
Camp Signaling Pathway ◽  
False Smut

AbstractRice false smut caused byUstilaginoidea virensis one of the widespread rice diseases across the globe in recent years, however, we know little about its molecular mechanism of infection. The cAMP signaling pathway functions directly in the development and formation of infectious structures to regulate the infection process in many pathogenic fungi. In order to investigate the role of the cAMP signaling pathway inU. virens, UvCap1, a cyclase-associated-protein homologous toSaccharomyces cerevisiaeSrv2 was identified. Three targeted deletion mutants of theUvCAP1gene were obtained with gene replacement strategy assisted with CRISPR-Cas9 system. TheUvCAP1deletion mutants showed defects in mycelial growth and conidial production. Inoculation experiments demonstrated that ΔUvcap1exhibited defects in pathogenicity. Compared with the wild-type strain, ΔUvcap1showed decreased tolerance to sorbitol and H2O2, and increased tolerance to NaCl, CFW and SDS, and the intracellular cAMP level was significantly reduced in ΔUvcap1. Yeast two-hybrid assay identified the interactions of UvCap1 with UvAc1 (adenylase cyclase), two Ras proteins (UvRas1 and UvRas2) and UvSte50. Taken together, as a component of cAMP signaling pathway, UvCap1 plays important roles in the development and pathogenicity ofU. virens.

scholarly journals Role of cAMP in Double Switch of Glucagon Secretion

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 896
Author(s):  
Jan Zmazek ◽  
Vladimir Grubelnik ◽  
Rene Markovič ◽  
Marko Marhl
Keyword(s):  
Glucose Metabolism ◽  
Signaling Pathway ◽  
Cell Model ◽  
Dominant Role ◽  
Glucagon Secretion ◽  
Camp Signaling ◽  
Independent Manner ◽  
Metabolic Switch ◽  
Camp Signaling Pathway ◽  
Double Switch

Glucose metabolism plays a crucial role in modulating glucagon secretion in pancreatic alpha cells. However, the downstream effects of glucose metabolism and the activated signaling pathways influencing glucagon granule exocytosis are still obscure. We developed a computational alpha cell model, implementing metabolic pathways of glucose and free fatty acids (FFA) catabolism and an intrinsically activated cAMP signaling pathway. According to the model predictions, increased catabolic activity is able to suppress the cAMP signaling pathway, reducing exocytosis in a Ca2+-dependent and Ca2+ independent manner. The effect is synergistic to the pathway involving ATP-dependent closure of KATP channels and consequent reduction of Ca2+. We analyze the contribution of each pathway to glucagon secretion and show that both play decisive roles, providing a kind of “secure double switch”. The cAMP-driven signaling switch plays a dominant role, while the ATP-driven metabolic switch is less favored. The ratio is approximately 60:40, according to the most recent experimental evidence.

scholarly journals A novel transcription factor UvCGBP1 regulates development and virulence of rice false smut fungus Ustilaginoidea virens

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1563-1579
Author(s):  
Xiaoyang Chen ◽  
Pingping Li ◽  
Hao Liu ◽  
Xiaolin Chen ◽  
Junbin Huang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Smut Fungus ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
False Smut

scholarly journals Quantitative Proteomics Analysis Reveals the Function of the Putative Ester Cyclase UvEC1 in the Pathogenicity of the Rice False Smut Fungus Ustilaginoidea virens

2021 ◽  
Vol 22 (8) ◽  
pp. 4069
Author(s):  
Xiaoyang Chen ◽  
Zhangxin Pei ◽  
Pingping Li ◽  
Xiabing Li ◽  
Yuhang Duan ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Fungal Pathogens ◽  
Protein Localization ◽  
Proteomics Data ◽  
Ustilaginoidea Virens ◽  
Rice False Smut ◽  
Oxidative Stresses ◽  
Tandem Mass Tag ◽  
Cyclase Domain ◽  
False Smut

Rice false smut is a fungal disease distributed worldwide and caused by Ustilaginoidea virens. In this study, we identified a putative ester cyclase (named as UvEC1) as being significantly upregulated during U. virens infection. UvEC1 contained a SnoaL-like polyketide cyclase domain, but the functions of ketone cyclases such as SnoaL in plant fungal pathogens remain unclear. Deletion of UvEC1 caused defects in vegetative growth and conidiation. UvEC1 was also required for response to hyperosmotic and oxidative stresses and for maintenance of cell wall integrity. Importantly, ΔUvEC1 mutants exhibited reduced virulence. We performed a tandem mass tag (TMT)-based quantitative proteomic analysis to identify differentially accumulating proteins (DAPs) between the ΔUvEC1-1 mutant and the wild-type isolate HWD-2. Proteomics data revealed that UvEC1 has a variety of effects on metabolism, protein localization, catalytic activity, binding, toxin biosynthesis and the spliceosome. Taken together, our findings suggest that UvEC1 is critical for the development and virulence of U. virens.

scholarly journals Assessment of cellular mechanisms contributing to cAMP compartmentalization in pulmonary microvascular endothelial cells

2012 ◽  
Vol 302 (6) ◽  
pp. C839-C852 ◽  
Author(s):  
Wei P. Feinstein ◽  
Bing Zhu ◽  
Silas J. Leavesley ◽  
Sarah L. Sayner ◽  
Thomas C. Rich
Keyword(s):  
Endothelial Cells ◽  
Adenylyl Cyclase ◽  
Signaling Pathway ◽  
Camp Signaling ◽  
Microvascular Endothelial Cells ◽  
Camp Production ◽  
Barrier Integrity ◽  
Pulmonary Microvascular Endothelial Cells ◽  
Camp Signaling Pathway ◽  
Microvascular Endothelial

Cyclic AMP signals encode information required to differentially regulate a wide variety of cellular responses; yet it is not well understood how information is encrypted within these signals. An emerging concept is that compartmentalization underlies specificity within the cAMP signaling pathway. This concept is based on a series of observations indicating that cAMP levels are distinct in different regions of the cell. One such observation is that cAMP production at the plasma membrane increases pulmonary microvascular endothelial barrier integrity, whereas cAMP production in the cytosol disrupts barrier integrity. To better understand how cAMP signals might be compartmentalized, we have developed mathematical models in which cellular geometry as well as total adenylyl cyclase and phosphodiesterase activities were constrained to approximate values measured in pulmonary microvascular endothelial cells. These simulations suggest that the subcellular localizations of adenylyl cyclase and phosphodiesterase activities are by themselves insufficient to generate physiologically relevant cAMP gradients. Thus, the assembly of adenylyl cyclase, phosphodiesterase, and protein kinase A onto protein scaffolds is by itself unlikely to ensure signal specificity. Rather, our simulations suggest that reductions in the effective cAMP diffusion coefficient may facilitate the formation of substantial cAMP gradients. We conclude that reductions in the effective rate of cAMP diffusion due to buffers, structural impediments, and local changes in viscosity greatly facilitate the ability of signaling complexes to impart specificity within the cAMP signaling pathway.

scholarly journals Increased Rho-kinase-mediated prostate contractions associated with impairment of β-adrenergic-cAMP-signaling pathway by chronic nitric oxide deficiency

2015 ◽  
Vol 758 ◽  
pp. 24-30 ◽  
Author(s):  
Fabiano Beraldi Calmasini ◽  
Luiz Osório Silveira Leiria ◽  
Marcos José Alves ◽  
Fernando Ricardo Báu ◽  
Eduardo Costa Alexandre ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Signaling Pathway ◽  
Rho Kinase ◽  
Camp Signaling ◽  
Camp Signaling Pathway ◽  
Nitric Oxide Deficiency

ChemInform Abstract: Ansellone A (I), a Sesterterpenoid Isolated from the Nudibranch Cadlina luteromarginata and the Sponge Phorbas sp., Activates the cAMP Signaling Pathway.

ChemInform ◽  
2010 ◽  
Vol 41 (48) ◽  
pp. no-no
Author(s):  
Julie Daoust ◽  
Angelo Fontana ◽  
Catherine E. Merchant ◽  
Nicole J. de Voogd ◽  
Brian O. Patrick ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Camp Signaling ◽  
Camp Signaling Pathway
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