Isolation and characterization of Schizosaccharomyces pombe mutants with defective NAD-dependent malic enzyme
1986 ◽
Vol 32
(6)
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pp. 481-486
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Keyword(s):
To obtain NAD-dependent malic enzyme mutants of Schizosaccharomyces pombe, a colony color indicator screening system was developed. Mutants defective for malic acid utilization (mau mutants) are yellow, while wild-type colonies are blue on the defined bromcresol green based indicator medium. NAD-dependent malic enzyme mutants were distinguished from other mau mutants by subsequent, starch gel electrophoresis, spectrophotometry, complementation tests, and intermediate pool analysis with cell-free extracts.
1963 ◽
Vol 16
(2)
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pp. 375
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1981 ◽
Vol 256
(23)
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pp. 12336-12342
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1982 ◽
Vol 257
(23)
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pp. 14492-14498
1990 ◽
Vol 46
(8)
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pp. 863-867
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2014 ◽
Vol 30
(3)
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pp. 377-390
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1985 ◽
Vol 5
(7)
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pp. 1543-1553
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