scholarly journals High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

2004 ◽  
Vol 22 (12) ◽  
Author(s):  
H. Hisano ◽  
Y. Kimoto ◽  
H. Hayakawa ◽  
J. Takeichi ◽  
T. Domae ◽  
...  
1997 ◽  
Vol 33 (4) ◽  
pp. 288-292 ◽  
Author(s):  
Winnie Teo ◽  
Prakash Lakshmanan ◽  
Prakash Kumar ◽  
Chong-Jin Goh ◽  
Sanjay Swarup

2001 ◽  
Vol 18 (1) ◽  
pp. 77-79 ◽  
Author(s):  
Ancharida AKARACHARANYA ◽  
Young-Eui CHOI ◽  
Tomonobu KUSANO ◽  
Atsuhiko SHINMYO ◽  
Hiroshi SANO

2018 ◽  
Vol 111 (1) ◽  
pp. 5 ◽  
Author(s):  
Avishek DATTA ◽  
Meutia ZAHARA ◽  
Patchareeya BOONKORKAEW ◽  
Abha MISHRA

<p><em>Phalaenopsis </em>orchids are one of the most beautiful flowering plants. The objective of this study was to identify the best plant growth regulator combination and medium for the growth and direct shoot formation from leaf explants of the hybrid <em>Phalaenopsis</em> ‘Pink’. Leaf tips segments from <em>in vitro </em>young plants were cultured on half-strength Murashige and Skoog (MS) and Vacin and Went (VW) media supplemented with different concentrations of auxin [α-napthaleneacetic acid (NAA)] and cytokinins [6-benzylaminopurine (BAP) or thidiazuron (TDZ)]. The explants that were cultured on 0 mg l<sup>–1</sup> NAA and 3 mg l<sup>–1</sup> BAP supplemented to half-strength MS medium formed shoots successfully within 10 weeks of culture with 5 % regenerants and 50 % survival frequency. The explants cultured on 0.5 mg l<sup>–1</sup> NAA and 1.5 mg l<sup>–1</sup> TDZ supplemented to half-strength MS medium developed calluses and shoots within 11 weeks of culture with 25 % regenerants and 90 % survival frequency. Future research needs to be directed to find out the shortest time of shoot regeneration to produce viable plants with a high survival frequency.</p>


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