PLANT REGENERATION FROM LEAF EXPLANTS AND EFFICIENT AGROBACTERIUM-MEDIATED TRANSFORMATION SYSTEM OF CHRYSANTHEMUM (DENDRANTHEMA GRANDIFLORUM)

2003 ◽  
pp. 333-338 ◽  
Author(s):  
S.Y. Seo ◽  
D.C. Choi ◽  
J.M. Kim ◽  
H.C. Lim ◽  
H.J. Kim ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Leaf Explants ◽  
Transformation System ◽  
Agrobacterium Mediated Transformation ◽  
Dendranthema Grandiflorum

An Agrobacterium-mediated transformation system of pyrethrum (Tanacetum cinerariifolium) based on leaf explants

2013 ◽  
Vol 150 ◽  
pp. 130-134 ◽  
Author(s):  
Jing Mao ◽  
Li-Yuan Cao ◽  
Ling-Fang Kong ◽  
Maarten A. Jongsma ◽  
Cai-Yun Wang
Keyword(s):  
Leaf Explants ◽  
Transformation System ◽  
Agrobacterium Mediated Transformation ◽  
Tanacetum Cinerariifolium

scholarly journals Efficient Agrobacterium-Mediated Transformation of the Commercial Hybrid Poplar Populus Alba × Populus glandulosa Uyeki

2019 ◽  
Vol 20 (10) ◽  
pp. 2594 ◽  
Author(s):  
Chengwei Song ◽  
Liang Lu ◽  
Yayu Guo ◽  
Huimin Xu ◽  
Ruili Li
Keyword(s):  
Genetic Transformation ◽  
Woody Plants ◽  
Functional Characterization ◽  
Hybrid Poplar ◽  
Leaf Explants ◽  
Populus Alba ◽  
Gus Gene ◽  
Transgenic Technology ◽  
Transformation System ◽  
Agrobacterium Mediated Transformation

Transgenic technology is a powerful tool for gene functional characterization, and poplar is a model system for genetic transformation of perennial woody plants. However, the poplar genetic transformation system is limited to a number of model genotypes. Herein, we developed a transformation system based on efficient Agrobacterium-mediated transformation for the hybrid poplar Populus Alba × Populus glandulosa Uyeki, which is a fast-growing poplar species that is suitably grown in the northern part of China. Importantly, we optimized many independent factors and showed that the transformation efficiency was improved significantly using juvenile leaf explants. Explants were infected by an Agrobacterium suspension with the OD600 = 0.6 for 15 min and then co-cultured in dark conditions for 3 days. Using the improved transformation system, we obtained the transgenic poplar with overexpression of β-glucuronidase (GUS) via direct organogenesis without callus induction. Furthermore, we analyzed the GUS gene in the transgenic poplars using PCR, qRT-PCR, and GUS staining. These analyses revealed that the GUS gene was efficiently transformed, and it exhibited various expression levels. Taken together, these results represent a simple, fast, and efficient transformation system of hybrid poplar plants. Our findings may facilitate future studies of gene functions in perennial woody plants and tree breeding via transgenic technology assisted design.

scholarly journals Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

2015 ◽  
Vol 25 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Kee Hwa Bae ◽  
Eui Soo Yoon
Keyword(s):  
Plant Regeneration ◽  
Seed Germination ◽  
Callus Induction ◽  
In Vitro Propagation ◽  
Ornamental Plants ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Temperature Treatment ◽  
In Vitro Plant Regeneration

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

scholarly journals A Regeneration and Agrobacterium-mediated Transformation System for Genetically Diverse Chrysanthemum Cultivars

1998 ◽  
Vol 123 (2) ◽  
pp. 189-194 ◽  
Author(s):  
John M. Sherman ◽  
James W. Moyer ◽  
Margaret E. Daub
Keyword(s):  
High Frequency ◽  
Callus Formation ◽  
Leaf Explants ◽  
Shoot Elongation ◽  
Neomycin Phosphotransferase ◽  
Cut Flower ◽  
Transformation System ◽  
High Concentration ◽  
Dendranthema Grandiflora ◽  
Rooting Medium

An efficient, high-frequency regeneration and Agrobacterium-mediated transformation system was developed allowing the genetic engineering of three chrysanthemum (Dendranthema grandiflora Tzvelev) cultivars: the formerly recalcitrant and economically important cut-flower mum `Polaris' and two potted mums, `Hekla' and `Iridon'. The regeneration protocol used leaf explants on a sequence of media with four hormone regimes. Explants were first cultured on an embryogenesis-type medium containing a high concentration of 2,4-D, which promoted callus formation. Shoot primordia were induced by culture on medium lacking 2,4-D, followed by shoot elongation on a high-cytokinin plus gibberellic acid medium. Finally, elongated shoots were rooted on a low-auxin rooting medium. Transformed plants of the three cultivars were obtained following co-culture of leaf explants with A. tumefaciens strain EHA 105 harboring the plasmid pBI121 containing genes for neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS). Stable transformation of the three cultivars was verified via GUS assays and Southern analysis.

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