Achieving desired plant growth regulator levels in liquid plant tissue culture media that include activated carbon

2005 ◽  
Vol 24 (4) ◽  
pp. 201-208 ◽  
Author(s):  
Stephen C. Van Winkle ◽  
Gerald S. Pullman
2012 ◽  
Vol 22 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Naruemol Kaewjampa ◽  
Kazuhiko Shimasaki ◽  
Syeda Jabun Nahar

Present authors investigated the effect of two biopolymer hyaluronic acid (HA9) and chitosan (Chitosan H), and N6-benzyladenine (BA) supplemented with modified MS on organogenesis in protocorm-like bodies (PLBs) of a Cymbidium cultivar. The percentages of PLB formation from cultures showed 100 % in all treatments except for control (82.7%), 0.1 mg/l HA9 (93.3%) and 10 mg/l Chitosan H (93.3%) treatments. Addition of 0.1 mg/l HA9 to culture medium was highly efficient for PLB multiplication. Both 10 mg/l HA9 and 1.0 mg/l BA significantly increased the number of shoots in cultures. The maximum percentage of shoot (100%) was obtained at 1.0 mg/l chitosan H. Increase in fresh weight showed higher values at BA treatments. Information gained from this study shows that hyaluronic acid (HA9) which is new additive for plant tissue culture act as growth regulator for hybrid Cymbidium micropropagation. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11261Plant Tissue Cult. & Biotech. 22(1): 59-64, 2012 (June)


2012 ◽  
Vol 47 (2) ◽  
pp. 161-166
Author(s):  
Salim Khan ◽  
Abdullah Al Maruf ◽  
Shahina Aktera ◽  
Ahashan Habiba ◽  
Tanjina Akhtar Banua ◽  
...  

Attempts have been made to find a substitute for agar as gelling agent. The study was conducted on commercially important crop plants like    potato varieties (Diamond and Granola) and Stevia. 'Isubgol', the mucilaginous husk, derived from Plantago ovata was successfully used as a gelling material in tissue culture media. The price of 'Isubgol husk' is cheaper than the conventionally used agar and it had reduced the price of gelling agent approximately by 47.5 % in plant tissue culture media. The use of 'Isubgol husk' along with agar can reduce the cost of gelling agents. The response from media gelled with Isubgol husk in potato and Stevia was similar to that from media solidified with agar. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11447 Bangladesh J. Sci. Ind. Res. 47(2), 161-166, 2012


2019 ◽  
Vol 12 (4) ◽  
pp. 1521
Author(s):  
R. Subbaiya ◽  
B. Aakash ◽  
A. Shanmugaraja ◽  
R. Devika ◽  
S. Chozhavendhan ◽  
...  

1990 ◽  
Vol 20 (2) ◽  
pp. 131-135 ◽  
Author(s):  
H. Wayne Elmore ◽  
Barbara Samples ◽  
Sanjeev Sharma ◽  
Marcia Harrison

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