scholarly journals Hyaluronic Acid Can be a New Plant Growth Regulator for Hybrid Cymbidium Micropropagation

2012 ◽  
Vol 22 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Naruemol Kaewjampa ◽  
Kazuhiko Shimasaki ◽  
Syeda Jabun Nahar
Keyword(s):  
Tissue Culture ◽  
Hyaluronic Acid ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Plant Growth Regulator ◽  
Culture Medium ◽  
Fresh Weight ◽  
Maximum Percentage

Present authors investigated the effect of two biopolymer hyaluronic acid (HA9) and chitosan (Chitosan H), and N6-benzyladenine (BA) supplemented with modified MS on organogenesis in protocorm-like bodies (PLBs) of a Cymbidium cultivar. The percentages of PLB formation from cultures showed 100 % in all treatments except for control (82.7%), 0.1 mg/l HA9 (93.3%) and 10 mg/l Chitosan H (93.3%) treatments. Addition of 0.1 mg/l HA9 to culture medium was highly efficient for PLB multiplication. Both 10 mg/l HA9 and 1.0 mg/l BA significantly increased the number of shoots in cultures. The maximum percentage of shoot (100%) was obtained at 1.0 mg/l chitosan H. Increase in fresh weight showed higher values at BA treatments. Information gained from this study shows that hyaluronic acid (HA9) which is new additive for plant tissue culture act as growth regulator for hybrid Cymbidium micropropagation. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11261Plant Tissue Cult. & Biotech. 22(1): 59-64, 2012 (June)

scholarly journals Peningkatan Induksi Kalus Embriogenik dan Konversi Embrio Somatik Kopi Robusta Klon BP 308

2017 ◽  
Vol 4 (3) ◽  
pp. 121
Author(s):  
Meynarti Sari Dewi Ibrahim ◽  
RR. Sri Hartati
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulator ◽  
Embryogenic Callus ◽  
Plant Growth Regulator ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Fresh Weight ◽  
Robusta Coffee ◽  
Randomized Design

<em>Robusta coffee </em>(Coffea canephora)<em> is a cross-pollinated plant, therefore vegetative propagation is necessary to ensure identical traits with parents, such as tissue culture techniques through somatic embryo. The study aimed to find the effect of plant growth regulator 2.4-D and thidiazuron in inducing embryogenic callus, by adding incision area on leaf explant, and to evaluate addition of GA<sub>3</sub> in increasing somatic embryo conversion. The study was conducted from December 2014 to June 2016 in the Tissue Culture Laboratory, IAARD, Bogor. The research consisted of 2 stages. Stage 1 used a complete randomized design of 2 factors; the first factor was a combination of plant growth regulator 2.4-D (1.0 and 2.0 mg/l) and thidiazuron (1.0; 3.0; and 5.0 mg/l), the second factor was leaf incision (slashed and unslashed). Stage 2 used a complete randomized design, with GA<sub>3 </sub>treatment at different concentrations (0.0; 0.5; and 1.0 mg/l). Observed variables were percentage of callus formation, fresh weight of callus, number of torpedoes, number of somatic embryos at cotyledon stage, and number of germinated embryo. The results showed growth regulatory treatments influenced the percentage of embryogenic callus formation and fresh weight of callus. Extra incision on leaf showed no effect in  embryogenic callus induction. Embryogenic callus inducted using 2.4-D 1.0 mg/l + thidiazuron 5.0 mg/l medium which  then regenerated in ½ MS medium added with kinetin 2 mg/l exhibited  the highest number of germination.  Adding GA<sub>3</sub> 0.1 mg/l in regeneration medium is recommended to increase somatic embryos of Robusta coffee BP 308 clone.</em>

scholarly journals Using Isothiazolone Biocides to Control Microbial and Fungal Contaminants in Plant Tissue Cultures

1998 ◽  
Vol 8 (4) ◽  
pp. 598-601 ◽  
Author(s):  
Randall P. Niedz
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Tissue ◽  
Culture Medium ◽  
Tissue Cultures ◽  
Fungal Contamination ◽  
Plant Tissue Cultures ◽  
Resistant Strains ◽  
Microbial Contaminants ◽  
Fungal Contaminants

Controlling bacterial and fungal contamination in plant tissue cultures is a serious problem. Antibiotics are currently used but are not always effective, can alter plant growth, and are costly, and resistant strains can result with extensive use. Plant preservative mixture (PPM) contains a mixture of two isothiazolones—methylchloroisothiazolinone and methylisothiazolinone, which are a class of broad-spectrum, widely used industrial biocides. The isothiazolones used in PPM are reported by the manufacturer to be nonphytotoxic at concentrations suitable for the prophylactic control of microbial contaminants in plant tissue cultures. Our results indicate that PPM can be routinely added to tissue culture medium to control air- and waterborne bacterial and fungal contaminants effectively.

scholarly journals Development of an efficient in vitro callus proliferation protocol for edible wild rhubarb (Rheum ribes L.)

2021 ◽  
Vol 20 (5) ◽  
pp. 119-126
Author(s):  
Burcu Tuncer
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Low Dose ◽  
Ms Medium ◽  
Fresh Weight ◽  
Hypocotyl Explants ◽  
Callus Proliferation ◽  
Callus Regeneration

Rheum ribes L. is a perennial wild species. Young shoots and flower bunches are freshly consumed, and root and rhizomes are generally used for medicinal purposes. The aim of the present study was to improve the callus proliferation protocol for R. ribes L. under in vitro conditions. For callus induction, hypocotyl explants taken from 14-day old plantlets germinated in Murashige and Skoog (MS) media were cultured in MS media with 9 plant growth regulator (PGR) combinations containing 6-benzylaminopurine (BAP) (2, 3, and 4 mg/L) + naphthylacetic acid (NAA) (0.1, 0.5, and 1 mg/L). Then, for callus proliferation, 4 PGR combinations containing NAA (0.2 mg/L) + thidiazuron (TDZ) (0.5, 1, 2, and 3 mg) were used in the first set of experiments, and 36 PGR combinations containing BAP (1, 2, 3, and 4 mg/L) + indole-3-butyric acid (IBA) (0.2, 0.5, and 1 mg/L), BAP (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L), and TDZ (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L) were used in the second set of experiments. At the end of the second set of experiments, the greatest callus regeneration ratios were obtained due to the combinations including BAP and IBA as well as the low-dose TDZ- (especially 1 mg/L) and NAA- (0.2, 0.5, 1 mg/L) combinations. Regarding callus fresh weights, TDZ + NAA combinations were found to be more successful. The greatest callus fresh weight (12.7 ±0.4 g) was obtained from MS medium supplemented with 2 mg/L TDZ and 0.2 mg/L NAA.

Plant hormones and plant growth regulators in plant tissue culture

1996 ◽  
Vol 32 (4) ◽  
pp. 272-289 ◽  
Author(s):  
Thomas Gaspar ◽  
Claire Kevers ◽  
Claude Penel ◽  
Hubert Greppin ◽  
David M. Reid ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Hormones ◽  
Plant Tissue Culture ◽  
Plant Tissue
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