Effect of basal medium, growth regulators and Phytagel concentration on initiation of embryogenic cultures from immature zygotic embryos of loblolly pine ( Pinus taeda L.)

1998 ◽  
Vol 17 (4) ◽  
pp. 298-301 ◽  
Author(s):  
X. Y. Li ◽  
F. H. Huang ◽  
E. E. Gbur Jr.
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Growth Regulators ◽  
Basal Medium ◽  
Zygotic Embryos ◽  
Embryogenic Cultures ◽  
Immature Zygotic Embryos ◽  
Pinus Taeda L

Regeneration of transgenic loblolly pine (Pinus taeda L.) from zygotic embryos transformed with Agrobacterium tumefaciens

Planta ◽  
2001 ◽  
Vol 213 (6) ◽  
pp. 981-989 ◽  
Author(s):  
Wei Tang ◽  
Ron Sederoff ◽  
Ross Whetten
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Zygotic Embryos ◽  
Pinus Taeda L

Initiation of embryogenic cultures and somatic embryo development in loblolly pine (Pinustaeda)

1990 ◽  
Vol 20 (6) ◽  
pp. 810-817 ◽  
Author(s):  
M. R. Becwar ◽  
R. Nagmani ◽  
S. R. Wann
Keyword(s):  
Somatic Embryo ◽  
Embryo Development ◽  
Loblolly Pine ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Basal Medium ◽  
Embryogenic Tissue ◽  
Zygotic Embryos ◽  
Embryogenic Cultures ◽  
Somatic Embryo Development

Immature zygotic embryo explants (isolated or with intact megagametophytes) from 10 loblolly pine (Pinustaeda L.) clones (7-34, 7-56, 11-9, 11-16, 11-25, 10-1003, 10-1007, 10-1011, 10-1018, and 10-1019) were surveyed for their potential to form embryogenic tissue from the suspensor region of zygotic embryos. After over 14 000 explants were cultured, embryogenic cultures were initiated from explants of 8 of the 10 clones; only explants from clones 11-25 and 10-1019 were not responsive. Embryogenic tissue was initiated from zygotic embryos with intact megagametophytes on MSG basal medium with no exogenous plant growth regulators or with 2–5 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0–1 mg/L N6-benzyladenine (BA). The highest initiation frequency (5%) was obtained from isolated zygotic embryos of clone 7-34 less than 0.5 mm in length just prior to cotyledon primordia development on DCR basal medium with 3 mg/L 2,4-D and 0.5 mg/L BA. Two types of embryogenic cultures were maintained on medium with 2,4-D and BA: (i) those that contained pre-embryonal masses of cells interspersed with unaggregated suspensorlike cells, but which rarely contained well-formed somatic embryos, and (ii) those that frequently contained well-formed somatic embryos. Somatic embryo development from both types of cultures progressed to a precotyledonary stage on medium with 2.6 mg/L abscisic acid.

scholarly journals A Consensus Map for Loblolly Pine (Pinus taeda L.). I. Construction and Integration of Individual Linkage Maps From Two Outbred Three-Generation Pedigrees

Genetics ◽  
1999 ◽  
Vol 151 (1) ◽  
pp. 321-330 ◽  
Author(s):  
Mitchell M Sewell ◽  
Bradley K Sherman ◽  
David B Neale
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Mapping Function ◽  
Genetic Maps ◽  
Linkage Data ◽  
Linkage Maps ◽  
Consensus Map ◽  
Pinus Taeda L

Abstract A consensus map for loblolly pine (Pinus taeda L.) was constructed from the integration of linkage data from two unrelated three-generation outbred pedigrees. The progeny segregation data from restriction fragment length polymorphism, random amplified polymorphic DNA, and isozyme genetic markers from each pedigree were recoded to reflect the two independent populations of parental meioses, and genetic maps were constructed to represent each parent. The rate of meiotic recombination was significantly greater for males than females, as was the average estimate of genome length for males {1983.7 cM [Kosambi mapping function (K)]} and females [1339.5 cM(K)]. The integration of individual maps allows for the synthesis of genetic information from independent sources onto a single consensus map and facilitates the consolidation of linkage groups to represent the chromosomes (n = 12) of loblolly pine. The resulting consensus map consists of 357 unique molecular markers and covers ∼1300 cM(K).

Long-term effects of stand density management and genotype on wood properties of loblolly pine (Pinus taeda L.) in the mid-South USA

2021 ◽  
Vol 491 ◽  
pp. 119176
Author(s):  
Michael A. Blazier ◽  
Thomas Hennessey ◽  
Laurence Schimleck ◽  
Scott Abbey ◽  
Ryan Holbrook ◽  
...  
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Stand Density ◽  
Wood Properties ◽  
Long Term Effects ◽  
Stand Density Management ◽  
Pinus Taeda L

scholarly journals Four Site-Preparation Techniques for Regenerating Pine-Hardwood Mixtures in the Piedmont

1997 ◽  
Vol 21 (3) ◽  
pp. 116-122 ◽  
Author(s):  
Thomas A. Waldrop
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Height Growth ◽  
Site Preparation ◽  
Site Conditions ◽  
Growing Seasons ◽  
Southern Appalachian ◽  
Medium Quality ◽  
Pinus Taeda L ◽  
Preparation Techniques

Abstract Four variations of the fell-and-burn technique, a system developed to produce mixed pine-hardwood stands in the Southern Appalachian Mountains, were compared in the Piedmont region. All variations of this technique successfully improved the commercial value of low-quality hardwood stands by introducing a pine component. After six growing seasons, loblolly pine (Pinus taeda L.) occupied the dominant crown position and oaks the codominant position in fell-and-burn treated stands on poor to medium quality sites. The precise timing of felling residual stems, as prescribed by the fell-and-burn technique, may be flexible because winter and spring felling produced similar results. Although summer site preparation burns reduced hardwood height growth by reducing the length of the first growing season, they did not improve pine survival or growth. Pines were as tall as hardwoods within four growing seasons in burned plots and within six growing seasons in unburned plots. Additional research is needed to determine the level or intensity of site preparation needed to establish pine-hardwood mixtures over a range of site conditions. South. J. Appl. For. 21(3):116-122.

scholarly journals Genotypic Stability Effects on Predicted Family Responses to Silvicultural Treatments in Loblolly Pine

1997 ◽  
Vol 21 (2) ◽  
pp. 84-89 ◽  
Author(s):  
Steven E. McKeand ◽  
Robert P. Crook ◽  
H. Lee Allen
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Growth Responses ◽  
Intensive Culture ◽  
Genotype By Environment ◽  
Silvicultural Treatments ◽  
Genotypic Stability ◽  
Superior Genotypes ◽  
Using Data ◽  
Pinus Taeda L

Abstract The lack of rank change in growth characteristics when open-pollinated families of loblolly pine (Pinus taeda L.) are planted on different sites in the Southeast has greatly simplified breeding for superior genotypes. Although family rank does not usually change, genotype by environment interactions (GxE) may be very important in operational deployment of families in regeneration programs. Using data from GxE trials and two site preparation-fertilization-herbicide trials, we estimated the growth that different families should achieve following application of these silvicultural practices. Better performing families tend to be most responsive to site changes (i.e. genetically unstable). Growth responses to silvicultural treatment will be overestimated if only the most responsive families are used in silvicultural research trials. Similarly, genetic gains will be overestimated if gain trials are planted on only the best sites or receive intensive culture. South. J. Appl. For. 21(2):84-89.

Somatic embryogenesis in loblolly pine ( Pinus taeda L.): improving culture initiation with abscisic acid and silver nitrate

2003 ◽  
Vol 22 (2) ◽  
pp. 85-95 ◽  
Author(s):  
G. S. Pullman ◽  
K. Namjoshi ◽  
Y. Zhang
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Silver Nitrate ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Culture Initiation ◽  
Pinus Taeda L
Sign in / Sign up

Export Citation Format

Share Document