Hairy root production in Arabidopsis thaliana : cotransformation with a promoter-trap vector results in complex T-DNA integration patterns

1999 ◽  
Vol 19 (2) ◽  
pp. 133-142 ◽  
Author(s):  
M. Karimi ◽  
M. Van Montagu ◽  
G. Gheysen
Keyword(s):  
Arabidopsis Thaliana ◽  
Hairy Root ◽  
Root Production ◽  
Dna Integration ◽  
Promoter Trap

scholarly journals Evaluation for Efficient Hairy Root Production Assessed by GFP-gene Expression in Tomato Cultivars Inoculated with Agrobacterium rhizogenes MAFF07-20001.

2003 ◽  
Vol 20 (3) ◽  
pp. 257-261
Author(s):  
Takeshi FUKUMOTO ◽  
Teruo NONOMURA ◽  
Yoshinori MATSUDA ◽  
Shin-ichirou KOMAKI ◽  
Nobuyuki MORIURA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Root Production ◽  
Gfp Gene ◽  
Tomato Cultivars

Optimizing Carrot Hairy Root Production for Monoxenic Culture of Arbuscular Mycorrhizal Fungi in Iran

2005 ◽  
Vol 6 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Y.R. Danesh . ◽  
E. Mohammadi Goltape . ◽  
A. Alizadeh . ◽  
M. Modarres Sanavy .
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Hairy Root ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Root Production ◽  
Monoxenic Culture

Characterization of a T-DNA promoter trap line of Arabidopsis thaliana uncovers a cryptic bi-directional promoter

Gene ◽  
2013 ◽  
Vol 524 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Pritu Pratibha ◽  
Sunil Kumar Singh ◽  
Isha Sharma ◽  
Ravi Kumar ◽  
Ramamurthy Srinivasan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Promoter Trap ◽  
Trap Line

scholarly journals Agrobacterium tumefaciens Transformation of the Radiation Hypersensitive Arabidopsis thaliana Mutants uvh1 and rad5

1998 ◽  
Vol 11 (11) ◽  
pp. 1136-1141 ◽  
Author(s):  
Jaesung Nam ◽  
Kirankumar S. Mysore ◽  
Stanton B. Gelvin
Keyword(s):  
Arabidopsis Thaliana ◽  
Agrobacterium Tumefaciens ◽  
Transformation Process ◽  
Stable Transformation ◽  
Wild Type ◽  
Dna Integration ◽  
Agrobacterium Tumefaciens Transformation ◽  
Inoculation Assay

The Arabidopsis thaliana mutants uvh1 and rad5, originally identified as radiation hypersensitive, were reported to be deficient in T-DNA integration based on the relative efficiencies of stable transformation and T-DNA transfer. We reassessed these mutants for susceptibility to transformation by Agrobacterium tumefaciens. The mutant rad5 showed a significant reduction in the efficiency of transient as well as stable transformation, compared with its wild-type progenitor. These data indicate that rad5 is blocked at a step in the transformation process prior to T-DNA integration. We additionally found, using both an in vitro root inoculation and an in vivo flower bolt inoculation assay, that the mutant uvh1 is as susceptible to A. tumefaciens-mediated transformation as is its wild-type progenitor, C10.

scholarly journals Sugar availability modulates polyisoprenoid and phytosterol profiles in Arabidopsis thaliana hairy root culture

2013 ◽  
Vol 1831 (2) ◽  
pp. 438-447 ◽  
Author(s):  
Adam Jozwiak ◽  
Magdalena Ples ◽  
Karolina Skorupinska-Tudek ◽  
Magdalena Kania ◽  
Marta Dydak ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture

scholarly journals Optimising production of carrot hairy roots

2004 ◽  
Vol 57 ◽  
pp. 77-80
Author(s):  
H.J. Ridgway ◽  
J. Kandula ◽  
A. Stewart
Keyword(s):  
New Zealand ◽  
Arbuscular Mycorrhizal Fungi ◽  
Hairy Root ◽  
Hairy Roots ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Root Production ◽  
Monoxenic Culture ◽  
Carrot Root ◽  
Positive Effect

This research describes the production of carrot hairy roots for monoxenic culture of New Zealand arbuscular mycorrhizal fungi (AMF) Induction of hairy roots on mature carrot root sections was carried out using Agrobacterium rhizogenes isolate A4T that had been grown in either LauriaBertani (/ acetosyringone) or Yeast Mannitol medium (24 or 48 h incubation) Results showed both methods could initiate hairy root production The source of the carrots was one of the most important factors with mature freshly harvested carrots showing better hairy root production compared to coolstored carrots Dissection of the cortex to expose the cambium had a positive effect on the least optimal treatment

T-DNA transfer and T-DNA integration efficiencies upon Arabidopsis thaliana root explant cocultivation and floral dip transformation

Planta ◽  
2013 ◽  
Vol 238 (6) ◽  
pp. 1025-1037 ◽  
Author(s):  
Rim Ghedira ◽  
Sylvie De Buck ◽  
Frédéric Van Ex ◽  
Geert Angenon ◽  
Ann Depicker
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Transfer ◽  
Dna Integration ◽  
Floral Dip ◽  
Root Explant
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