Genomics and transcriptomics analyses provide insights into the cold adaptation strategies of an Antarctic bacterium, Cryobacterium sp. SO1

Polar Biology ◽  
2021 ◽  
Author(s):  
C. P. Teoh ◽  
P. Lavin ◽  
D. J. H. Lee ◽  
M. González-Aravena ◽  
N. Najimudin ◽  
...  
2011 ◽  
Vol 4 ◽  
pp. PRI.S5383 ◽  
Author(s):  
M.V. Jagannadham ◽  
S. Saranya

The proteins of an Antarctic bacterium Pseudomonas syringae Lz4W, identified earlier by different membrane protein preparation methods, were combined together and the redundant identities removed. In total, 1479 proteins including 148 outer membrane proteins from this bacterium were predicted by the algorithm PSORTb3.0. A detailed analysis on their subcellular localization was undertaken which was determined using TMHMM, TMB-hunt and BOMP. A comparison of PSORTb predicted outer membrane proteins with BOMP, revealed that most of the proteins predicted by the former, contained β–barrels in the outer membranes. A comparative analysis of PSORTb, TMHMM and TMB-hunt reveals that most of the outer membranes proteins of this bacterium could be identified using this approach. Thus, by using a combination of biochemical and different bioinformatics algorithms, the membrane proteins of P. syringae are analyzed. In particular, PSORTb results are compared and supported by other algorithms, to improve the strength of OM proteins prediction. Several proteins, having an important role in cold adaptation of the organism, could also be identified.


PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192559 ◽  
Author(s):  
Florencia C. Benforte ◽  
Maria A. Colonnella ◽  
Martiniano M. Ricardi ◽  
Esmeralda C. Solar Venero ◽  
Leonardo Lizarraga ◽  
...  

2021 ◽  
Vol 7 (7) ◽  
pp. 528
Author(s):  
Nur Athirah Yusof ◽  
Noor Haza Fazlin Hashim ◽  
Izwan Bharudin

Psychrophilic organisms possess several adaptive strategies which allow them to sustain life at low temperatures between −20 to 20 °C. Studies on Antarctic psychrophiles are interesting due to the multiple stressors that exist on the permanently cold continent. These organisms produce, among other peculiarities, cold-active enzymes which not only have tremendous biotechnological potential but are valuable models for fundamental research into protein structure and function. Recent innovations in omics technologies such as genomics, transcriptomics, proteomics and metabolomics have contributed a remarkable perspective of the molecular basis underpinning the mechanisms of cold adaptation. This review critically discusses similar and different strategies of cold adaptation in the obligate psychrophilic yeast, Glaciozyma antarctica PI12 at the molecular (genome structure, proteins and enzymes, gene expression) and physiological (antifreeze proteins, membrane fluidity, stress-related proteins) levels. Our extensive studies on G. antarctica have revealed significant insights towards the innate capacity of- and the adaptation strategies employed by this psychrophilic yeast for life in the persistent cold. Furthermore, several cold-active enzymes and proteins with biotechnological potential are also discussed.


Extremophiles ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Paula M. Tribelli ◽  
Magdalena Pezzoni ◽  
María Gabriela Brito ◽  
Nahuel V. Montesinos ◽  
Cristina S. Costa ◽  
...  

2019 ◽  
pp. 77-104 ◽  
Author(s):  
Karla Diana Infante Ramírez ◽  
Ana Minerva Arce Ibarra

The main objective of this study was to analyze local perceptions of climate variability and the different adaptation strategies of four communities in the southern Yucatán Peninsula, using the Social-Ecological System (SES) approach. Four SESs were considered: two in the coastal zone and two in the tropical forest zone. Data were collected using different qualitative methodological tools (interviews, participant observation, and focal groups) and the information collected from each site was triangulated. In all four sites, changes in climate variability were perceived as “less rain and more heat”. In the tropical forest (or Maya) zone, an ancestral indigenous weather forecasting system, known as “Xook k’íin” (or “las cabañuelas”), was recorded and the main activity affected by climate variability was found to be slash-and burn farming or the milpa. In the coastal zone, the main activities affected are fishing and tourism. In all the cases analyzed, local climate change adaptation strategies include undertaking alternative work, and changing the calendar of daily, seasonal and annual labor and seasonal migration. The population of all four SESs displayed concern and uncertainty as regards dealing with these changes and possible changes in the future.


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