Three-dimensional true FISP for high-resolution imaging of the whole brain

2003 ◽  
Vol 13 (7) ◽  
pp. 1577-1582 ◽  
Author(s):  
B. Schmitz ◽  
T. Hagen ◽  
W. Reith
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
High Resolution Imaging ◽  
Whole Brain ◽  
True Fisp ◽  
Resolution Imaging

scholarly journals Plastic embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging

2017 ◽  
Vol 8 (8) ◽  
pp. 3583 ◽  
Author(s):  
Yadong Gang ◽  
Xiuli Liu ◽  
Xiaojun Wang ◽  
Qi Zhang ◽  
Hongfu Zhou ◽  
...  
Keyword(s):  
High Resolution ◽  
Large Volume ◽  
Three Dimensional ◽  
High Resolution Imaging ◽  
Plastic Embedding ◽  
Resolution Imaging

scholarly journals Three-Dimensional High-Resolution Imaging of Gold Nanorods Uptake in Sentinel Lymph Nodes

Nano Letters ◽  
2011 ◽  
Vol 11 (7) ◽  
pp. 2938-2943 ◽  
Author(s):  
Yeongri Jung ◽  
Roberto Reif ◽  
Yaguang Zeng ◽  
Ruikang K. Wang
Keyword(s):  
High Resolution ◽  
Lymph Nodes ◽  
Gold Nanorods ◽  
Three Dimensional ◽  
Sentinel Lymph Nodes ◽  
High Resolution Imaging ◽  
Resolution Imaging

In Vivo Three-Dimensional High-Resolution Imaging of Rodent Retina with Spectral-Domain Optical Coherence Tomography

2007 ◽  
Vol 48 (4) ◽  
pp. 1808 ◽  
Author(s):  
Marco Ruggeri ◽  
Hassan Wehbe ◽  
Shuliang Jiao ◽  
Giovanni Gregori ◽  
Maria E. Jockovich ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
High Resolution ◽  
Three Dimensional ◽  
Spectral Domain ◽  
High Resolution Imaging ◽  
Optical Coherence ◽  
Resolution Imaging

scholarly journals 3D-printed microplate inserts for long term high-resolution imaging of live brain organoids

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Mariana Oksdath Mansilla ◽  
Camilo Salazar-Hernandez ◽  
Sally L. Perrin ◽  
Kaitlin G. Scheer ◽  
Gökhan Cildir ◽  
...  
Keyword(s):  
High Resolution ◽  
Cell Tracking ◽  
Culture Conditions ◽  
Significant Advance ◽  
High Resolution Imaging ◽  
Whole Brain ◽  
3D Printed ◽  
Resolution Imaging ◽  
Brain Organoid

Abstract Background Organoids are a reliable model used in the study of human brain development and under pathological conditions. However, current methods for brain organoid culture generate tissues that range from 0.5 to 2 mm of size, which need to be constantly agitated to allow proper oxygenation. The culture conditions are, therefore, not suitable for whole-brain organoid live imaging, required to study developmental processes and disease progression within physiologically relevant time frames (i.e. days, weeks, months). Results Here we designed 3D-printed microplate inserts adaptable to standard 24 multi-well plates, which allow the growth of multiple organoids in pre-defined and fixed XYZ coordinates. This innovation facilitates high-resolution imaging of whole-cerebral organoids, allowing precise assessment of organoid growth and morphology, as well as cell tracking within the organoids, over long periods. We applied this technology to track neocortex development through neuronal progenitors in brain organoids, as well as the movement of patient-derived glioblastoma stem cells within healthy brain organoids. Conclusions This new bioengineering platform constitutes a significant advance that permits long term detailed analysis of whole-brain organoids using multimodal inverted fluorescence microscopy.

scholarly journals Three-dimensional imaging of mitochondrial cristae complexity using cryo-soft X-ray tomography

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Carla C. Polo ◽  
Miriam H. Fonseca-Alaniz ◽  
Jian-Hua Chen ◽  
Axel Ekman ◽  
Gerry McDermott ◽  
...  
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Linear Attenuation Coefficient ◽  
High Resolution Imaging ◽  
Mitochondrial Matrix ◽  
Morphological Parameter ◽  
Mitochondrial Membranes ◽  
X Ray ◽  
Resolution Imaging ◽  
Neuronal Disorders

AbstractMitochondria are dynamic organelles that change morphology to adapt to cellular energetic demands under both physiological and stress conditions. Cardiomyopathies and neuronal disorders are associated with structure-related dysfunction in mitochondria, but three-dimensional characterizations of the organelles are still lacking. In this study, we combined high-resolution imaging and 3D electron density information provided by cryo-soft X-ray tomography to characterize mitochondria cristae morphology isolated from murine. Using the linear attenuation coefficient, the mitochondria were identified (0.247 ± 0.04 µm−1) presenting average dimensions of 0.90 ± 0.20 µm in length and 0.63 ± 0.12 µm in width. The internal mitochondria structure was successfully identified by reaching up the limit of spatial resolution of 35 nm. The internal mitochondrial membranes invagination (cristae) complexity was calculated by the mitochondrial complexity index (MCI) providing quantitative and morphological information of mitochondria larger than 0.90 mm in length. The segmentation to visualize the cristae invaginations into the mitochondrial matrix was possible in mitochondria with MCI ≥ 7. Altogether, we demonstrated that the MCI is a valuable quantitative morphological parameter to evaluate cristae modelling and can be applied to compare healthy and disease state associated to mitochondria morphology.

scholarly journals Nano-laminography for three-dimensional high-resolution imaging of flat specimens

2013 ◽  
Vol 8 (05) ◽  
pp. C05006-C05006 ◽  
Author(s):  
L Helfen ◽  
F Xu ◽  
H Suhonen ◽  
L Urbanelli ◽  
P Cloetens ◽  
...  
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
High Resolution Imaging ◽  
Resolution Imaging
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