Culture of yeast cells immobilized by alginate-chitosan microcapsules in aqueous-organic solvent biphasic system

S-licarbazepine was synthesized by asymmetric reduction of oxcarbazepine in organic solvent/phosphate buffer biphasic system with Bacillus anthracis CGMCC No.12337 as catalyst. Effects of many factors on reduction were studied. Dibutyl phthalate/phosphate buffer was selected as the optimal biphasic system for reduction. The optimum reduction conditions are as follows: volume ratio of dibutyl phthalate and phosphate buffer 1:1, 30 g/L iospropanol as co-substrate, phosphate buffer pH 5, substrate concentration 7.92 mmol/L, cell concentration 30 g/L, 32°C，180 rpm. The conversion and enantiometric excess of S-licarbazepine reached 97.32% and 99.80%. An efficient method for synthesis of S-licarbazepine was provided.

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Asymmetric Hydrolysis of Styrene Oxide Catalyzed by Mung Bean Epoxide Hydrolase in Organic Solvent/Buffer Biphasic System

CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION) ◽

10.3724/sp.j.1088.2011.10606 ◽

2014 ◽

Vol 32 (9) ◽

pp. 1557-1563 ◽

Cited By ~ 1

Author(s):

Wenjing CHEN ◽

Wenyong LOU ◽

Xiaoting WANG ◽

Minhua ZONG

Keyword(s):

Organic Solvent ◽

Mung Bean ◽

Epoxide Hydrolase ◽

Styrene Oxide ◽

Biphasic System ◽

Hydrolysis Of

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Peptide synthesis enzymatically catalyzed in a biphasic system: Water-water-immiscible organic solvent

Biotechnology and Bioengineering ◽

10.1002/bit.260230210 ◽

1981 ◽

Vol 23 (2) ◽

pp. 355-360 ◽

Cited By ~ 37

Author(s):

Anatole N. Semenov ◽

Ilya V. Berezin ◽

Karel Martinek

Keyword(s):

Organic Solvent ◽

Peptide Synthesis ◽

Biphasic System ◽

System Water

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Hybrid liquid biphasic system for cell disruption and simultaneous lipid extraction from microalgae Chlorella sorokiniana CY-1 for biofuel production

Biotechnology for Biofuels ◽

10.1186/s13068-019-1591-8 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 5

Author(s):

Guo Yong Yew ◽

Kit Wayne Chew ◽

Marlinda Abdul Malek ◽

Yeek-Chia Ho ◽

Wei-Hsin Chen ◽

...

Keyword(s):

Cell Wall ◽

Organic Solvent ◽

Lipid Extraction ◽

Research Work ◽

Volume Ratio ◽

Single Step ◽

Biofuel Production ◽

Biphasic System ◽

One Step ◽

Extraction Processes

Abstract Background The extraction of lipids from microalgae requires a pretreatment process to break the cell wall and subsequent extraction processes to obtain the lipids for biofuels production. The multistep operation tends to incur high costs and are energy intensive due to longer process operations. This research work applies the combination of radicals from hydrogen peroxide with an organic solvent as a chemical pretreatment method for disrupting the cell wall of microalgae and simultaneously extracting lipids from the biomass in a one-step biphasic solution. Result Several parameters which can affect the biphasic system were analyzed: contact time, volume of solvent, volume ratio, type of organic solvent, biomass amount and concentration of solvents, to extract the highest amount of lipids from microalgae. The results were optimized and up to 83.5% of lipid recovery yield and 94.6% of enhancement was successfully achieved. The results obtain from GC-FID were similar to the analysis of triglyceride lipid standard. Conclusion The profound hybrid biphasic system shows great potential to radically disrupt the cell wall of microalgae and instantaneously extract lipids in a single-step approach. The lipids extracted were tested to for its comparability to biodiesel performance.

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