A yield-independent, 15N-isotope dilution method to estimate legume symbiotic dependence without a non-N2-fixing reference plant

1996 ◽  
Vol 23 (2) ◽  
pp. 196-199
Author(s):  
P. M. Chalk ◽  
C. J. Smith ◽  
P. Hopmans ◽  
S. D. Hamilton
Keyword(s):  
Isotope Dilution ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
15N Isotope Dilution ◽  
15N Isotope ◽  
Reference Plant ◽  
Symbiotic Dependence

15N-Isotope dilution as a measure of dinitrogen fixation by Azospirillum brasilense associated with maize

1980 ◽  
Vol 58 (1) ◽  
pp. 21-24 ◽  
Author(s):  
R. J. Rennie
Keyword(s):  
Isotope Dilution ◽  
Azospirillum Brasilense ◽  
Dinitrogen Fixation ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
15N Isotope Dilution ◽  
Fixed Nitrogen ◽  
15N Isotope ◽  
Maize Rhizosphere

The classical difference method (based on Kjeldahl determinations) and the 15N-isotope dilution method to measure dinitrogen fixation by Azospirillum brasilense associated with maize in vitro were compared. Both methods resulted in an accurate measure of the amount of Azospirilluin-fixed nitrogen found in the maize plant; 12.6% of the maize nitrogen was derived from the atmosphere. When sucrose, malate, or succinate was added as a carbon source to encourage dinitrogen fixation in the maize rhizosphere, atmosphere-derived nitrogen in the maize increased to 19.0, 28.0, and 38.0% of the total nitrogen (the remainder coming from seed nitrogen).

Sign in / Sign up

Export Citation Format

Share Document