Suitability and use of the 15N-isotope dilution method to estimate nitrogen fixation by actinorhizal shrubs

2000 ◽  
Vol 136 (1-3) ◽  
pp. 85-95 ◽  
Author(s):  
Matt D Busse
Keyword(s):  
Nitrogen Fixation ◽  
Isotope Dilution ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
15N Isotope Dilution ◽  
15N Isotope

15N-Isotope dilution as a measure of dinitrogen fixation by Azospirillum brasilense associated with maize

1980 ◽  
Vol 58 (1) ◽  
pp. 21-24 ◽  
Author(s):  
R. J. Rennie
Keyword(s):  
Isotope Dilution ◽  
Azospirillum Brasilense ◽  
Dinitrogen Fixation ◽  
Isotope Dilution Method ◽  
Dilution Method ◽  
15N Isotope Dilution ◽  
Fixed Nitrogen ◽  
15N Isotope ◽  
Maize Rhizosphere

The classical difference method (based on Kjeldahl determinations) and the 15N-isotope dilution method to measure dinitrogen fixation by Azospirillum brasilense associated with maize in vitro were compared. Both methods resulted in an accurate measure of the amount of Azospirilluin-fixed nitrogen found in the maize plant; 12.6% of the maize nitrogen was derived from the atmosphere. When sucrose, malate, or succinate was added as a carbon source to encourage dinitrogen fixation in the maize rhizosphere, atmosphere-derived nitrogen in the maize increased to 19.0, 28.0, and 38.0% of the total nitrogen (the remainder coming from seed nitrogen).

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