15N-Isotope dilution as a measure of dinitrogen fixation by Azospirillum brasilense associated with maize
Keyword(s):
The classical difference method (based on Kjeldahl determinations) and the 15N-isotope dilution method to measure dinitrogen fixation by Azospirillum brasilense associated with maize in vitro were compared. Both methods resulted in an accurate measure of the amount of Azospirilluin-fixed nitrogen found in the maize plant; 12.6% of the maize nitrogen was derived from the atmosphere. When sucrose, malate, or succinate was added as a carbon source to encourage dinitrogen fixation in the maize rhizosphere, atmosphere-derived nitrogen in the maize increased to 19.0, 28.0, and 38.0% of the total nitrogen (the remainder coming from seed nitrogen).
1987 ◽
Vol 19
(4)
◽
pp. 405-409
◽
Keyword(s):
2002 ◽
Vol 1
(1)
◽
pp. 17-22
◽
Keyword(s):
1996 ◽
Vol 23
(2)
◽
pp. 196-199
◽
Keyword(s):
2000 ◽
Vol 136
(1-3)
◽
pp. 85-95
◽
Keyword(s):
1998 ◽
pp. 307-312
◽
Keyword(s):
2001 ◽
Vol 33
(3)
◽
pp. 409-412
◽
Keyword(s):