Comparison of the Mineral and Nutraceutical Profiles of Elephant Garlic (Allium ampeloprasum L.) Grown in Organic and Conventional Fields of Valdichiana, a Traditional Cultivation Area of Tuscany, Italy

In the Valdichiana area (Tuscany, Italy) an ancient native landrace of elephant garlic (Allium ampeloprasum L.), locally known as “Aglione della Valdichiana”, has long been cultivated. The aim of this study was to check whether there are differences in the mineral and nutraceutical profiles of the Aglione della Valdichiana cultivated conventionally and organically. Based on the analysis by ICP-MS of a wide array of major, minor, essential, and non-essential trace elements as well as rare earth elements, and the evaluation of the content of polyphenols, flavonoids, antioxidants, soluble proteins, soluble sugars, and starch, as well as the weight and water content, it was concluded that differences in the mineral and nutraceutical profiles of organically and conventionally grown bulbs were very limited. Only a statistically (p < 0.05) higher concentration of Cd (+2620%), Co (+113%), Mn (+55%), Rb (+180%), and Sb (+180%), as well as glucose (+37%) in conventionally cultivated bulbs emerged. Cadmium was the only element slightly higher than in the “reference plant,” but with a negligible risk (three orders of magnitude lower) for human health based on consumption. It is concluded that we failed to find evidence of healthier food or a higher nutraceutical quality for organically cultivated elephant garlic.

Assessment of Soybean Resistance to Leaf-feeding Insect, Spodoptera litura

One of soybean problem in Indonesia is leaf damage caused by the Spodoptera litura. The aim of the study was to identify the resistance of soybean genotypes againts army worm based on the preference index and morphophysiological characters. 150 soybean genotypes were grown under field condition from February to May 2018 in Malang (East Java, Indonesia). The preference test was conducted in the laboratory using G100H as resistant reference plant to armyworm. The result showed that the preference index was ranged from 0.09-1.82. A total of 105 genotypes showed a low preference index (resistant), 39 genotypes showed a high preference index (susceptible), and a genotype has similar preference index with the reference plant (has equal resistance with G100H). Based on the preference index and morphophysiological characters, 150 soybean genotypes can be grouped into eight clusters. A genotype from cluster VIII (Anjasmoro/Rajabasa-41) has characteristics of low leaf water content, dense leaf trichomes, and the preference index of 0.14. Those characteristics could be the attributes of the soybean genotypes that is resistant to S. litura. The Anjasmoro/Rajabasa-41 was recommended to be cultivated in Indonesian, or may be used as a gene source in the breeding program for S. litura resistance

ANALYTICAL SIMULATION OF A PWR USING MELCOR

After the two most significant nuclear accidents in history – the Chernobyl Reactor Four explosion in Ukraine(1986) and the Fukushima Daiichi accident in Japan (2011) –, the Final Safety Analysis Report (FSAR) included a new chapter (19) dedicated to the Probabilistic Safety Assessment (PSA) and Severe Accident Analysis (SAA), covering accidents with core melting. FSAR is the most important document for licensing of siting, construction, commissioning and operation of a nuclear power plant. In the USA, the elaboration of the FSAR chapter 19 is according to the review and acceptance criteria described in the NUREG-0800 and U.S. Nuclear Regulatory Commission (NRC) Regulatory Guide (RG) 1.200. The same approach is being adopted in Brazil by National Nuclear Energy Commission (CNEN). Therefore, the FSAR elaboration requires a detailed knowledge of severe accident phenomena and an analysis of the design vulnerabilities to the severe accidents, as provided in a PSA – e.g., the identification of the initiating events involving significant Core Damage Frequency (CDF) are made in the PSA Level 1. As part of the design and certification activities of a plant of reference, the Laboratory of Risk Analysis, Evaluating and Management (LabRisco), located in the University of São Paulo (USP), Brazil, has been preparing a group of specialists to model the progression of severe accidents in Pressurized Water Reactors (PWR), to support the CNEN regulatory expectation – since Brazilian Nuclear Power Plants (NPP), i.e., Angra 1, 2 and 3, have PWR type, the efforts of the CNEN are concentrated on accidents at this type of reactor. The initial investigation objectives were on completing the detailed input data for a PWR cooling system model using the U.S. NRC MELCOR 2.2 code, and on the study of the reference plant equipment behavior – by comparing this model results and the reference plant normal operation main parameters, as modeled with RELAP5/MOD2 code.

MODELING LOFW IN A PWR USING MELCOR

The Probabilistic Safety Assessment (PSA) is part of a Nuclear Power Plant (NPP) licensing process. It considers the elaboration and updating of probabilistic models that estimate the risk associated to the operation, allowing the risk monitoring from the design to the plant decommissioning, for both operational as regulatory activities. The PSA identifies those components or plant systems whose unavailability contributes significantly to the Core Damage Frequency (CDF) and to the Large Early Release Frequency (LERF) of radioactive material. Based on the PSA Level 1 results for a reference plant under design, the Analysis, Evaluating and Risk Management Laboratory (LabRisco), located in the University of São Paulo (USP), Brazil, started the analytical investigation of severe accident phenomena using the US Nuclear Regulatory Commission (NRC) MELCOR2.2 code – focusing on the qualification of a group of specialists who will subsidize a PSA Level 2 for the same plant. This PSA Level 1 shows that the accident with large CDF contribution is the Loss of Feed Water Accident (LOFW). Therefore, the initial objective of the investigation was to model the progression of severe accidents during a LOFW for the reference Pressurized Water Reactor (PWR) and to analyze the response of the plant under these accident scenarios. During the course of the hypothetical LOFW in the reference plant, hydrogen was generated – by a reaction between the high temperature steam water and the fuel-cladding inside the reactor pressure vessel (RPV) but not representing a serious threat to the RPV integrity.

High-Efficiency Bioenergy Carbon Capture Integrating Chemical Looping Combustion with Oxygen Uncoupling and a Large Cogeneration Plant

Bioenergy with CO2 capture and storage (BECCS) is a promising negative emission technology (NET). When using sustainably produced biomass as fuel, BECCS allows the production of power and heat with negative CO2 emissions. The main technical challenges hindering the deployment of BECCS technologies include energy penalties associated with the capture process. This work evaluates the performance of an advanced CO2 capture technology, chemical looping with oxygen uncoupling (CLOU), in conjunction with biomass-fired combined heat and power (CHP) generation. Results from a MATLAB/Simulink reactor model were incorporated in a plant and integration model developed in a commercial process simulation software to quantify the key performance indicators of the CLOU-integrated CHP plant. Both energy and exergy analysis were conducted. The results show a remarkably low efficiency penalty of 0.7% compared to a conventional reference plant, and a high carbon capture efficiency of 97%. The low efficiency penalty is due to the high moisture and hydrogen contents of the biomass, and the separation of combustion products and excess air streams in the CLOU process; these together provide an opportunity to recover a significant amount of heat by flue gas condensation at a higher temperature level than what is possible in a conventional boiler. The condensing heat recovery yields an 18 MW generator power increase (3 MW loss in net power output) for the CLOU plant; in the reference plant with conventional boiler, the same scheme could achieve an increase of 9 MW (generator) and a decrease of 8 MW (net).

CRISPR/Cas9-Mediated Mutagenesis of RCO in Cardamine hirsuta

The small crucifer Cardamine hirsuta bears complex leaves divided into leaflets. This is in contrast to its relative, the reference plant Arabidopsis thaliana, which has simple leaves. Comparative studies between these species provide attractive opportunities to study the diversification of form. Here, we report on the implementation of the CRISPR/Cas9 genome editing methodology in C. hirsuta and with it the generation of novel alleles in the RCO gene, which was previously shown to play a major role in the diversification of form between the two species. Thus, genome editing can now be deployed in C. hirsuta, thereby increasing its versatility as a model system to study gene function and evolution.

CamRegBase: a gene regulation database for the biofuel crop, Camelina sativa

Camelina is an annual oilseed plant from the Brassicaceae family that is gaining momentum as a biofuel winter cover crop. However, a significant limitation in further enhancing its utility as a producer of oils that can be used as biofuels, jet fuels or bio-based products is the absence of a repository for all the gene expression and regulatory information that is being rapidly generated by the community. Here, we provide CamRegBase (https://camregbase.org/) as a one-stop resource to access Camelina information on gene expression and co-expression, transcription factors, lipid associated genes and genome-wide orthologs in the close-relative reference plant Arabidopsis. We envision this as a resource of curated information for users, as well as a repository of new gene regulation information.

Design and Characterization of a Pressure Sensor Based on FBG on Steel Substrate

In this paper a new optical, Fiber Bragg Grating (FBG) based, pressure sensor is presented. The sensor adopts a stainless steel membrane with a FBG sensor attached and is thought to be used in a multi parametric multi-sensor system aimed at the detection of water leakages in potable water networks. The sensor has been experimentally characterized on a reference plant in the range of pressure 0–6 bar. Main metrological characteristics of the lab-scale prototype developed are: a sensitivity of 0.314 nm/bar, an accuracy of about 39 mbar and a resolution of about 1.5 mbar.

Marking RNA: m6A writers, readers, and functions in Arabidopsis

N6-methyladenosine (m6A) emerges as an important modification in eukaryotic mRNAs. m6A has first been reported in 1974, and its functional significance in mammalian gene regulation and importance for proper development have been well established. An arsenal of writer, eraser, and reader proteins accomplish deposition, removal, and interpretation of the m6A mark, resulting in dynamic function. This led to the concept of an epitranscriptome, the compendium of RNA species with chemical modification of the nucleobases in the cell, in analogy to the epigenome. While m6A has long been known to also exist in plant mRNAs, proteins involved in m6A metabolism have only recently been detected by mutant analysis, homology search, and mRNA interactome capture in the reference plant Arabidopsis thaliana. Dysregulation of the m6A modification causes severe developmental abnormalities of leaves and roots and altered timing of reproductive development. Furthermore, m6A modification affects viral infection. Here, we discuss recent progress in identifying m6A sites transcriptome-wide, in identifying the molecular players involved in writing, removing, and reading the mark, and in assigning functions to this RNA modification in A. thaliana. We highlight similarities and differences to m6A modification in mammals and provide an outlook on important questions that remain to be addressed.

An Overview of Evapotranspiration Guide for Irrigated Crops in Turkey

The “Action Plan for the Water Use in Agriculture Activation Program” in the Tenth Five-Year Development Plan, prepared by The Ministry of Development for the period 2014-2018, is one of the most important indicators of the increasing importance of water resources and irrigation. It’s published according to Blaney-Criddle method with the data of the Crop Evapotranspiration Guideline in 1982, for a guide to be updated by General Directorate of Agricultural Research And Policies (TAGEM) and General Directorate of State Hydraulic Works (DSI) in 2013, using the Food and Agriculture Organization (FAO) Penman Monteith method. While DSİ undertakes the calculation of reference plant water consumption (ET0), effective precipitation and irrigation water requirement of this update, TAGEM is responsible for the compilation of information obtained from irrigation researches, calculation of plant coefficient (kc) values, publication of guides in written and electronic media realized. In this study, five guides (Balıkesir, Çanakkale, Edirne, Kocaeli and Tekirdağ) in three different climatic regions (Akdeniz Ardı, Marmara and İç Geçit-Marmara) were used in this guide, which the two institutions jointly organize and update with intensive effort, TUIK 2012-2016 considering the data, the county made on the basis of agriculture and untreated plants, guide place whether to get cases examined, the importance and aspects to be developed contacts have tried to reveal.