The effect of the β-emitting yttrium-90 citrate on the dose–response of dicentric chromosomes in human lymphocytes: a basis for biological dosimetry after radiosynoviorthesis

Abstract Ionizing radiation is extensively utilized in various applications; however, it can lead to significant harm to living systems. In this regard, the radiation absorbed dose is usually evaluated by performing biological dosimetry and physical reconstruction of exposure scenarios. But, this is costly, time-consuming, and maybe impractical for a biodosimetry lab to perform biological dosimetry. This study aimed to assess the applicability and reliability of the Geant4-DNA toolkit as a simulation approach to construct a reliable dose-response curve for biodosimetry purposes as an appropriate substitution for experimental measurements. In this matter, the total number of double-strand breaks (DSBs), due to different doses of low LET radiation qualities on DNA molecules, was calculated and converted to the values of dicentric chromosomes using a mechanistic model of cellular response. Then, the number of dicentric chromosomes induced by 200 kVp X-rays were modified by using a semi-empirical scaling factor for compensating the restriction of simulation code to consider what can happen in a real cell. Next, the trend of dicentrics for 137Cs and 60Co were calculated and modified by the above scaling factor. Finally, the dose-response curves for these gamma sources compared to several published experiments. The suggested calibration curves for 137Cs and 60Co followed a linear quadratic equation: Ydic = 0.0054 (± 0.0133) - 0.0089 (± 0.0212) × D + 0.0568 (± 0.0051) × D2 and Ydic = 0.0052 (± 0.0128) - 0.00568 (± 0.0203) × D + 0.0525 (± 0.0049) × D2 respectively. They revealed a satisfactory agreement with the experimental data reported by others. The Geant4 program developed in this work could provide an appropriate tool for predicting the dose-response (calibration) curve for biodosimetry purposes.

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The 60Co gamma ray dose-response for chromosomal aberrations in human lymphocytes analysed by FISH; applicability to biological dosimetry

International Journal of Radiation Biology ◽

10.1080/095530099139368 ◽

1999 ◽

Vol 75 (10) ◽

pp. 1215-1222 ◽

Cited By ~ 28

Author(s):

P. Finnon, J. E. Moquet, A. A. Edwards

Keyword(s):

Chromosomal Aberrations ◽

Dose Response ◽

Gamma Ray ◽

Human Lymphocytes ◽

Biological Dosimetry ◽

60Co Gamma

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Linear Dose Response of Acrocentric Chromosome Associations (Aca) To Gamma Irradiation In Human Lymphocytes

10.21203/rs.3.rs-1116997/v1 ◽

2021 ◽

Author(s):

Ravindra M Samarth ◽

Puneet Gandhi ◽

Nabo Kumar Chaudhury

Keyword(s):

Gamma Irradiation ◽

Dose Response ◽

Acrocentric Chromosome ◽

Linear Trend ◽

Human Lymphocytes ◽

Regression Equation ◽

Dicentric Chromosomes ◽

Healthy Donors ◽

Chromosome Associations ◽

Dose Dependent Increase

Abstract Purpose: The frequency of acrocentric chromosome associations (ACA) was studied to determine the possible dose-response relation with low doses of gamma irradiation in lymphocytes. Methods: Peripheral blood collected from three healthy donors were irradiated with 0, 0.1, 0.25, 0.5, 0.75, and 1 Gy gamma radiation. Chromosomal preparations were made after 48 hrs culture as per the standard guidelines. Results: The average number of ACA and ACA % were increased significantly with an increase in a dose. The D-G and D-D type of association was most prominent and showed a dose-dependent increase. The ACA frequency in irradiated lymphocytes showed an increase concerning the dose. The fitted regression equation was y=0.4759x+0.1663 (R2=0.9635; p=0.0005). An assessment of dicentric chromosomes (DC) was carried for the same slides. The correlation curve was prepared for ACA frequencies versus DC frequencies, resulting in a regression equation as y=8.659x+0.2.37 (R2=0.8275; p=0.0119). Conclusion: Our results showed an increase in frequencies of ACA in irradiated lymphocytes with an increase in radiation dose and followed a similar linear trend with DC frequency, thus, ACA may serve as a candidate cytogenetic biomarker for radiation biodosimetry especially for low radiation doses.

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Biological dosimetry after yttrium-90 citrate colloid radiosynoviorthesis

Nuklearmedizin ◽

10.1055/s-0038-1625224 ◽

2006 ◽

Vol 45 (05) ◽

pp. 223-228 ◽

Cited By ~ 5

Author(s):

M. Voth ◽

P. Lengsfeld ◽

G. Stephan ◽

E. Schmid ◽

R. Klett

Keyword(s):

Knee Joint ◽

Radiation Effect ◽

Whole Body ◽

Radiation Parameter ◽

Characteristic Radiation ◽

Biological Dosimetry ◽

Dicentric Chromosomes ◽

Blood Specimens ◽

Yttrium 90

Summary:Radiosynoviorthesis (RSO) with the β-particle-emitting nuclide yttrium-90 is an established concept for the treatment of persistent synovitis of the knee joint. The aim of this study was to investigate the biological radiation effect on the basis of a characteristic radiation parameter. Patients, methods: After RSO procedures with yttrium-90 citrate colloid and subsequent immobilisation of the knee, blood specimens of 10 patients were collected immediately before RSO and 11 to 13 days after the intervention. The yield of dicentric chromosomes in the lymphocytes was determined exclusively in metaphases of the first cell cycle in vitro. In addition, activity leakage was measured by wholebody bremsstrahlung-scintigraphy. Results: No statistically significant increase in the number of dicentric chromosomes (26 before treatment and 34 after treatment) in 20 192 cells analyzed from the 20 blood samples could be found as a result of RSO. However, the analysis of at least 1000 cells per blood sample demonstrates a tendency for a biological radiation effect in the blood of patients on the basis of this characteristic radiation parameter. Two of the 10 RSO patients had undergone a second RSO using yttrium- 90 citrate, whereby one patient displayed activity transport out of the knee joint, amounting to 6 MBq. Only for him a radiation effect (about 130 mGy per single RSO) could be calculated by biological dosimetry. Conclusion: Since in general, based on the analysis of dicentric chromosomes in at least 1000 lymphocytes per individual, detection limits for groups of persons after long-term exposures to low-LET radiation of 50-100 mGy are possible, we assume that RSO with yttrium-90 should be associated with a low whole-body radiation exposure.

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