Relative contribution of different transmembrane segments to the CFTR chloride channel pore

2013 ◽  
Vol 466 (3) ◽  
pp. 477-490 ◽  
Author(s):  
Wuyang Wang ◽  
Yassine El Hiani ◽  
Hussein N. Rubaiy ◽  
Paul Linsdell
Keyword(s):  
Chloride Channel ◽  
Channel Pore ◽  
Transmembrane Segments ◽  
Relative Contribution

TMEM16E (GDD1) Exhibits Protein Instability and Distinct Characteristics in Chloride Channel/Pore Forming Ability

2013 ◽  
Vol 229 (2) ◽  
pp. 181-190 ◽  
Author(s):  
Ta To Tran ◽  
Kei Tobiume ◽  
Chikara Hirono ◽  
Shinichi Fujimoto ◽  
Kuniko Mizuta ◽  
...  
Keyword(s):  
Chloride Channel ◽  
Channel Pore ◽  
Protein Instability

Thiocyanate as a probe of the cystic fibrosis transmembrane conductance regulator chloride channel pore

2001 ◽  
Vol 79 (7) ◽  
pp. 573-579 ◽  
Author(s):  
Paul Linsdell
Keyword(s):  
Cystic Fibrosis ◽  
Steady State ◽  
Mole Fraction ◽  
Chloride Channel ◽  
Theory Model ◽  
Anion Binding ◽  
Rate Theory ◽  
Patch Clamp Recording ◽  
Channel Pore ◽  
Mammalian Cell Lines

Immediately following exposure to thiocyanate (SCN–)-containing solutions, the cystic fibrosis conductance regulator Cl– channel exhibits high unitary SCN– conductance and anomalous mole fraction behaviour, suggesting the presence of multiple anion binding sites within the channel pore. However, under steady-state conditions SCN– conductance is very low. Here I show, using patch clamp recording from CFTR-transfected mammalian cell lines, that under steady-state conditions neither SCN– conductance nor SCN– permeability show anomalous mole fraction behaviour. Instead, SCN– conductance, permeability, and block of Cl– permeation can all be reproduced by a rate theory model that assumes only a single intrapore anion binding site. These results suggest that under steady-state conditions the interaction between SCN– and the CFTR channel pore can be understood by a simple model whereby SCN– ions enter the pore more easily than Cl–, and bind within the pore more tightly than Cl–. The implications of these findings for investigating and understanding the mechanism of anion permeation are discussed.Key words: chloride channel, permeation, anion binding, multi-ion pore behaviour, rate theory model.

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