Ultrastructural localization of the NADPH-diaphorase activity in the Leydig cells of aging mice

2001 ◽  
Vol 203 (5) ◽  
pp. 383-391 ◽  
Author(s):  
R. Ruffoli ◽  
Maria Anita Giambelluca ◽  
Franco Giannessi ◽  
Paola Soldani ◽  
Lucia Grasso ◽  
...  
Keyword(s):  
Leydig Cells ◽  
Ultrastructural Localization ◽  
Nadph Diaphorase ◽  
Diaphorase Activity

Ultrastructural Localization of NADPH-Diaphorase Activity in the Endothelial Cells of Human Nasal Respiratory Mucosa

1999 ◽  
Vol 13 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Riccardo Ruffoli ◽  
Bruno Fattori ◽  
Paola Soldani ◽  
Maria A. Giambelluca ◽  
Francesco Giannessi
Keyword(s):  
Endothelial Cells ◽  
Ultrastructural Localization ◽  
Nadph Diaphorase ◽  
Respiratory Mucosa ◽  
Diaphorase Activity

Cytochemical localisation of the NADPH diaphorase activity in the Leydig cells of the mouse

1998 ◽  
Vol 109 (3) ◽  
pp. 241-248 ◽  
Author(s):  
F. Giannessi ◽  
R. Ruffoli ◽  
M. A. Giambelluca ◽  
G. Morelli ◽  
F. Menchini Fabris
Keyword(s):  
Leydig Cells ◽  
Nadph Diaphorase ◽  
Diaphorase Activity

256 NADPH-diaphorase activity in the paraventricular and sypraoptic nuclei in postnatal rat

1997 ◽  
Vol 28 ◽  
pp. S49
Author(s):  
YiMu Yang ◽  
Hitoshi Ozawa ◽  
Kazunari Yuri ◽  
Mitsuhiro Kawata
Keyword(s):  
Nadph Diaphorase ◽  
Diaphorase Activity

Distribution of NADPH-diaphorase activity in the central nervous system of the young and adult land snail Megalobulimus abbreviatus

2010 ◽  
Vol 42 (5) ◽  
pp. 307-313 ◽  
Author(s):  
P. Rigon ◽  
J. de Castilhos ◽  
C.G. Molina ◽  
D.M. Zancan ◽  
M. Achaval
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Land Snail ◽  
Nadph Diaphorase ◽  
Diaphorase Activity ◽  
The Central Nervous System

5-Hydroxytryptophan activates colonic myenteric neurons and propulsive motor function through 5-HT4 receptors in conscious mice

2007 ◽  
Vol 292 (1) ◽  
pp. G419-G428 ◽  
Author(s):  
L. Wang ◽  
V. Martínez ◽  
H. Kimura ◽  
Y. Taché
Keyword(s):  
Motor Function ◽  
Colonic Motility ◽  
Distal Colon ◽  
Nadph Diaphorase ◽  
Maximal Response ◽  
Myenteric Neurons ◽  
Diaphorase Activity ◽  
Colonic Motor ◽  
Different Populations ◽  
Fos Expression

Serotonin [5-hydroxytryptamine (5-HT)] acts as a modulator of colonic motility and secretion. We characterized the action of the 5-HT precursor 5-hydroxytryptophan (5-HTP) on colonic myenteric neurons and propulsive motor activity in conscious mice. Fos immunoreactivity (IR), used as a marker of neuronal activation, was monitored in longitudinal muscle/myenteric plexus whole mount preparations of the distal colon 90 min after an intraperitoneal injection of 5-HTP. Double staining of Fos IR with peripheral choline acetyltransferase (pChAT) IR or NADPH-diaphorase activity was performed. The injection of 5-HTP (0.5, 1, 5, or 10 mg/kg ip) increased fecal pellet output and fluid content in a dose-related manner, with a peak response observed within the first 15 min postinjection. 5-HTP (0.5–10 mg/kg) dose dependently increased Fos expression in myenteric neurons, with a maximal response of 9.9 ± 1.0 cells/ganglion [ P < 0.05 vs. vehicle-treated mice (2.3 ± 0.6 cells/ganglion)]. There was a positive correlation between Fos expression and fecal output. Of Fos-positive ganglionic cells, 40 ± 4% were also pChAT positive and 21 ± 5% were NADPH-diaphorase positive in response to 5-HTP, respectively. 5-HTP-induced defecation and Fos expression were completely prevented by pretreatment with the selective 5-HT4 antagonist RS-39604. These results show that 5-HTP injected peripherally increases Fos expression in different populations of cholinergic and nitrergic myenteric neurons in the distal colon and stimulates propulsive colonic motor function through 5-HT4 receptors in conscious mice. These findings suggest an important role of activation of colonic myenteric neurons in the 5-HT4 receptor-mediated colonic propulsive motor response.

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