Characterization of pECL18 and pKPN2: a proposed pathway for the evolution of two plasmids that carry identical genes for a Type II restriction-modification system

2002 ◽  
Vol 267 (2) ◽  
pp. 171-178 ◽  
Author(s):  
M. Zakharova ◽  
I. Beletskaya ◽  
M. Denjmukhametov ◽  
T. Yurkova ◽  
L. Semenova ◽  
...  
Keyword(s):  
Type Ii ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals Functional characterization of the type II PamI restriction-modification system derived from plasmid pAMI7 of Paracoccus aminophilusJCM 7686

2011 ◽  
Vol 324 (1) ◽  
pp. 56-63 ◽  
Author(s):  
Lukasz Dziewit ◽  
Katarzyna Kuczkowska ◽  
Marcin Adamczuk ◽  
Monika Radlinska ◽  
Dariusz Bartosik
Keyword(s):  
Functional Characterization ◽  
Type Ii ◽  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals Low-level expression of the Type II restriction–modification system confers potent bacteriophage resistance in Escherichia coli

DNA Research ◽  
2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Karolina Wilkowska ◽  
Iwona Mruk ◽  
Beata Furmanek-Blaszk ◽  
Marian Sektas
Keyword(s):  
Dna Methyltransferase ◽  
Host Bacterium ◽  
Type Ii ◽  
Potential Conflict ◽  
Modification System ◽  
Biological Assays ◽  
Restriction Modification System ◽  
Highly Sensitive ◽  
System Maintenance ◽  
Restriction Modification

Abstract Restriction–modification systems (R–M) are one of the antiviral defense tools used by bacteria, and those of the Type II family are composed of a restriction endonuclease (REase) and a DNA methyltransferase (MTase). Most entering DNA molecules are usually cleaved by the REase before they can be methylated by MTase, although the observed level of fragmented DNA may vary significantly. Using a model EcoRI R–M system, we report that the balance between DNA methylation and cleavage may be severely affected by transcriptional signals coming from outside the R–M operon. By modulating the activity of the promoter, we obtained a broad range of restriction phenotypes for the EcoRI R–M system that differed by up to 4 orders of magnitude in our biological assays. Surprisingly, we found that high expression levels of the R–M proteins were associated with reduced restriction of invading bacteriophage DNA. Our results suggested that the regulatory balance of cleavage and methylation was highly sensitive to fluctuations in transcriptional signals both up- and downstream of the R–M operon. Our data provided further insights into Type II R–M system maintenance and the potential conflict within the host bacterium.

scholarly journals Species-Specific Type II Restriction-Modification System of Xylella fastidiosa Temecula1

2010 ◽  
Vol 76 (12) ◽  
pp. 4092-4095 ◽  
Author(s):  
Ayumi Matsumoto ◽  
Michele M. Igo
Keyword(s):  
Escherichia Coli ◽  
Transformation Efficiency ◽  
Xylella Fastidiosa ◽  
Type Ii ◽  
Modification System ◽  
Restriction Modification System ◽  
Species Specific ◽  
Restriction Modification

ABSTRACT The transformation efficiency of Xylella fastidiosa can be increased by interfering with restriction by the strain-specific type II system encoded by the PD1607 and PD1608 genes. Here, we report results for two strategies: in vitro methylation using M.SssI and isolation of DNA from an Escherichia coli strain expressing the methylase PD1607.

Cloning and characterization of the BamHI restriction modification system

Gene ◽  
1988 ◽  
Vol 74 (1) ◽  
pp. 13 ◽  
Author(s):  
J.E. Brooks ◽  
J.S. Benner ◽  
K.R. Silber ◽  
D.F. Heiter ◽  
L.A. Sznyter ◽  
...  
Keyword(s):  
Modification System ◽  
Restriction Modification System ◽  
Restriction Modification

scholarly journals MmeI: a minimal Type II restriction-modification system that only modifies one DNA strand for host protection

2008 ◽  
Vol 36 (20) ◽  
pp. 6558-6570 ◽  
Author(s):  
Richard D. Morgan ◽  
Tanya K. Bhatia ◽  
Lindsay Lovasco ◽  
Theodore B. Davis
Keyword(s):  
Type Ii ◽  
Modification System ◽  
Host Protection ◽  
Restriction Modification System ◽  
Minimal Type ◽  
Dna Strand ◽  
Restriction Modification
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