Species-Specific Type II Restriction-Modification System of Xylella fastidiosa Temecula1

ABSTRACT The transformation efficiency of Xylella fastidiosa can be increased by interfering with restriction by the strain-specific type II system encoded by the PD1607 and PD1608 genes. Here, we report results for two strategies: in vitro methylation using M.SssI and isolation of DNA from an Escherichia coli strain expressing the methylase PD1607.

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Cloning the KpnI restriction-modification system in Escherichia coli

Gene ◽

10.1016/0378-1119(91)90014-3 ◽

1991 ◽

Vol 97 (1) ◽

pp. 97-102 ◽

Cited By ~ 6

Author(s):

Alan W. Hammond ◽

Gary F. Gerard ◽

Deb K. Chatterjee

Keyword(s):

Escherichia Coli ◽

Modification System ◽

Restriction Modification System ◽

Restriction Modification

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Plasmid pEC156, a Naturally Occurring Escherichia coli Genetic Element That Carries Genes of the EcoVIII Restriction-Modification System, Is Mobilizable among Enterobacteria

PLoS ONE ◽

10.1371/journal.pone.0148355 ◽

2016 ◽

Vol 11 (2) ◽

pp. e0148355 ◽

Cited By ~ 9

Author(s):

Olesia Werbowy ◽

Tadeusz Kaczorowski

Keyword(s):

Escherichia Coli ◽

Genetic Element ◽

Modification System ◽

Restriction Modification System ◽

Naturally Occurring ◽

Restriction Modification

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Type II DNA restriction-modification system and an endonuclease from the ruminal bacterium Fibrobacter succinogenes S85.

Journal of Bacteriology ◽

10.1128/jb.174.16.5275-5283.1992 ◽

1992 ◽

Vol 174 (16) ◽

pp. 5275-5283 ◽

Cited By ~ 17

Author(s):

S F Lee ◽

C W Forsberg ◽

A M Gibbins

Keyword(s):

Fibrobacter Succinogenes ◽

Type Ii ◽

Ruminal Bacterium ◽

Modification System ◽

Restriction Modification System ◽

Dna Restriction ◽

Restriction Modification

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Characterization of the archaeal, plasmid-encoded type II restriction-modification system MthTI from Methanobacterium thermoformicicum THF: homology to the bacterial NgoPII system from Neisseria gonorrhoeae.

Journal of Bacteriology ◽

10.1128/jb.174.17.5719-5726.1992 ◽

1992 ◽

Vol 174 (17) ◽

pp. 5719-5726 ◽

Cited By ~ 27

Author(s):

J Nölling ◽

W M de Vos

Keyword(s):

Neisseria Gonorrhoeae ◽

Type Ii ◽

Modification System ◽

Restriction Modification System ◽

Restriction Modification

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Low-level expression of the Type II restriction–modification system confers potent bacteriophage resistance in Escherichia coli

DNA Research ◽

10.1093/dnares/dsaa003 ◽

2020 ◽

Vol 27 (1) ◽

Author(s):

Karolina Wilkowska ◽

Iwona Mruk ◽

Beata Furmanek-Blaszk ◽

Marian Sektas

Keyword(s):

Dna Methyltransferase ◽

Host Bacterium ◽

Type Ii ◽

Potential Conflict ◽

Modification System ◽

Biological Assays ◽

Restriction Modification System ◽

Highly Sensitive ◽

System Maintenance ◽

Restriction Modification

Abstract Restriction–modification systems (R–M) are one of the antiviral defense tools used by bacteria, and those of the Type II family are composed of a restriction endonuclease (REase) and a DNA methyltransferase (MTase). Most entering DNA molecules are usually cleaved by the REase before they can be methylated by MTase, although the observed level of fragmented DNA may vary significantly. Using a model EcoRI R–M system, we report that the balance between DNA methylation and cleavage may be severely affected by transcriptional signals coming from outside the R–M operon. By modulating the activity of the promoter, we obtained a broad range of restriction phenotypes for the EcoRI R–M system that differed by up to 4 orders of magnitude in our biological assays. Surprisingly, we found that high expression levels of the R–M proteins were associated with reduced restriction of invading bacteriophage DNA. Our results suggested that the regulatory balance of cleavage and methylation was highly sensitive to fluctuations in transcriptional signals both up- and downstream of the R–M operon. Our data provided further insights into Type II R–M system maintenance and the potential conflict within the host bacterium.

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The EcoKI Type I Restriction-Modification System in Escherichia coli Affects but Is Not an Absolute Barrier for Conjugation

Journal of Bacteriology ◽

10.1128/jb.02418-14 ◽

2014 ◽

Vol 197 (2) ◽

pp. 337-342 ◽

Cited By ~ 27

Author(s):

Louise Roer ◽

Frank M. Aarestrup ◽

Henrik Hasman

Keyword(s):

Escherichia Coli ◽

Rapid Evolution ◽

Type I ◽

Modification System ◽

Major Barrier ◽

Exogenous Dna ◽

Content Type ◽

Restriction Modification System ◽

K 12 ◽

Restriction Modification

The rapid evolution of bacteria is crucial to their survival and is caused by exchange, transfer, and uptake of DNA, among other things. Conjugation is one of the main mechanisms by which bacteria share their DNA, and it is thought to be controlled by varied bacterial immune systems. Contradictory results about restriction-modification systems based on phenotypic studies have been presented as reasons for a barrier to conjugation with and other means of uptake of exogenous DNA. In this study, we show that inactivation of the R.EcoKI restriction enzyme in strainEscherichia coliK-12 strain MG1655 increases the conjugational transfer of plasmid pOLA52, which carriers two EcoKI recognition sites. Interestingly, the results were not absolute, and uptake of unmethylated pOLA52 was still observed in the wild-type strain (with an intacthsdRgene) but at a reduction of 85% compared to the uptake of the mutant recipient with a disruptedhsdRgene. This leads to the conclusion that EcoKI restriction-modification affects the uptake of DNA by conjugation but is not a major barrier to plasmid transfer.

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