The endogenous Drosophila melanogaster retrovirus gypsy can propagate in Drosophila hydei cells

2001 ◽  
Vol 264 (5) ◽  
pp. 588-594 ◽  
Author(s):  
B. V. Syomin ◽  
L. I. Fedorova ◽  
S. A. Surkov ◽  
Y. V. Ilyin
Keyword(s):  
Drosophila Melanogaster ◽  
Drosophila Hydei

Structure and expression of histone H3.3 genes in Drosophila melanogaster and Drosophila hydei

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 586-600 ◽  
Author(s):  
Anna S. Akhmanova ◽  
Petra C. T. Bindels ◽  
Jie Xu ◽  
Koos Miedema ◽  
Hannie Kremer ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Sequence Similarity ◽  
Expression Patterns ◽  
Alternative Polyadenylation ◽  
Cdna Clones ◽  
Drosophila Hydei ◽  
Histone H3.3 ◽  
Somatic Tissues ◽  
Polyadenylation Sites ◽  
Limited Sequence

We demonstrate that in Drosophila melanogaster the histone H3.3 replacement variant is encoded by two genes, H3.3A and H3.3B. We have isolated cDNA clones for H3.3A and cDNA and genomic clones for H3.3B. The genes encode exactly the same protein but are widely divergent in their untranslated regions (UTR). Both genes are expressed in embryos and adults; they are expressed in the gonads as well as in somatic tissues of the flies. However, only one of them, H3.3A, shows strong testes expression. The 3′ UTR of the H3.3A gene is relatively short (~250 nucleotides (nt)). H3.3B transcripts can be processed at several polyadenylation sites, the longest with a 3′ UTR of more than 1500 nt. The 3′ processing sites, preferentially used in the gonads and somatic tissues, are different. We have also isolated the Drosophila hydei homologues of the two H3.3 genes. They are quite similar to the D. melanogaster genes in their expression patterns. However, in contrast to their vertebrate counterparts, which are highly conserved in their noncoding regions, the Drosophila genes display only limited sequence similarity in these regions.Key words: H3.3 histone variant, Drosophila, sequence comparison, alternative polyadenylation, testis expression.

Regulated expression of a Drosophila melanogaster heat shock locus after stable integration in a Drosophila hydei cell line

1985 ◽  
Vol 5 (11) ◽  
pp. 3208-3213
Author(s):  
J H Sinclair ◽  
S E Saunders ◽  
J F Burke ◽  
J H Sang
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cell Line ◽  
Stable Integration ◽  
Drosophila Hydei ◽  
Regulated Expression ◽  
Shock Locus

DNA-mediated cotransformation has been used to transfer a Drosophila melanogaster heat shock locus into cultured Drosophila hydei cells by use of the copia-based selectable vector pCV2gpt and of pMH10A, a cloned 87A7 heat shock locus encoding a mutant heat shock protein (hsp). Transformed lines contain between 50 and 200 copies of both plasmids, each separately organized as a head-to-tail concatemer which is stably maintained in the transformed lines. Exposure of the cotransformants to heat shock temperatures induces the regulated expression of the hsp RNA and the mutant hsp in all the lines analyzed.

scholarly journals VARIATIONS IN THE NUMBER OF THE Y CHROMOSOMAL rRNA GENES IN DROSOPHILA HYDEI

Genetics ◽  
1976 ◽  
Vol 82 (1) ◽  
pp. 25-34
Author(s):  
W Kunz
Keyword(s):  
Drosophila Melanogaster ◽  
Y Chromosome ◽  
X Chromosome ◽  
Nucleolus Organizer ◽  
Rrna Genes ◽  
Wild Type ◽  
Drosophila Hydei

ABSTRACT The number of rRNA cistrons is measured by filter saturation hybridization in different stocks of D. hydei, where the wild-type X chromosome has one nucleolus organizer (NO) and the wild-type Y has two separated NO's. (see PDF) females having no X chromosomal NO show an rDNA content exceeding that of a Y chromosome. An even greater increase in the rRNA cistron number is measured in two translocation stocks where the (see PDF) is combined with one half of a Y and, therefore, each stock contains only one of the two Y chromosomal NO's. But when the same Y fragments are brought together with a wild-type X chromosome they lose about one-half of their rRNA cistrons within one generation. Males with two complementary Y fragments but having no X chromosomal NO show a considerably higher rDNA content than the (see PDF) females, although both are equal in respect of their NO number. Consideration is given to related phenomena in Drosophila melanogaster.

scholarly journals Y chromosome loops in Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 120 (4) ◽  
pp. 1015-1034
Author(s):  
S Bonaccorsi ◽  
C Pisano ◽  
F Puoti ◽  
M Gatti
Keyword(s):  
Drosophila Melanogaster ◽  
Y Chromosome ◽  
Primary Spermatocyte ◽  
Maximum Size ◽  
Specific Region ◽  
Meiotic Metaphase ◽  
Fixation Procedure ◽  
Physical Size ◽  
Drosophila Hydei ◽  
Large Clusters

Abstract Primary spermatocyte nuclei of fixed testes of Drosophila melanogaster exhibit three large clusters of thread-like structures, each consisting of two long, continuous, loop-shaped filaments. No comparable intranuclear structures are observed in spermatogonia, secondary spermatocytes or spermatids. The threads begin to form in young spermatocytes, grow throughout spermatocyte development, reach their maximum size in mature spermatocytes and disintegrate prior to meiotic metaphase I. The presence of each cluster of threads depends upon the presence of a specific region of the Y chromosome; when this region is deleted the cluster is absent, and when it is duplicated the cluster is also duplicated. Together these observations strongly suggest that these structures represent giant Y chromosome lampbrush-like loops analogous to those described in Drosophila hydei. Two antibodies, one polyclonal and one monoclonal, differentially react with the three loops of D. melanogaster. Moreover, two of these loops are specifically stained by Giemsa at pH 10. By indirect immunofluorescence with these antibodies followed by Giemsa staining, each loop can be unambiguously identified and its presence and normality readily assessed. This enabled us to perform fine mapping experiments to determine the relationships between the Y chromosome fertility factors and the loops. The loop-forming sites map within the kl-5, kl-3 and ks-1 fertility factors. Regions h3 and h21 of the Y chromosome correspond to the loop-forming sites of kl-5 and ks-1, respectively. Each of these regions contains about 1300 kb of DNA and spans about one-third of its locus. The loop-forming site of the kl-3 locus is coextensive with region h7-h9 which contains about 4300 kb of DNA and corresponds to the minimum physical size of this locus. These data suggest that each loop is an integral part of a different fertility factor, representing the cytological manifestation of its activity in primary spermatocytes. The kl-2, kl-1 and ks-2 fertility regions do not produce any visible intranuclear structure and do not affect the kl-5, kl-3 and ks-1 loops. Thus, these loci may either not form loops at all or produce loop-like structures that we are unable to see because they are physically minute, destroyed by our fixation procedure, or both.

Transfection of Drosophila melanogaster Transposable Elements into the Drosophila hydei Cell Line

1987 ◽  
pp. 251-263
Author(s):  
TADASHI MIYAKE ◽  
NAOMI MAE ◽  
TADAYOSHI SHIBA ◽  
SHUNZO KONDO ◽  
MANABU TAKAHISA ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Transposable Elements ◽  
Cell Line ◽  
Drosophila Hydei

Interspecific sequence comparison of the muscle-myosin heavy-chain genes from Drosophila hydei and Drosophila melanogaster

1994 ◽  
Vol 39 (4) ◽  
pp. 357-368 ◽  
Author(s):  
Koos Miedema ◽  
Harry Harhangi ◽  
Stef Mentzel ◽  
Monique Wilbrink ◽  
Anna Akhmanova ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Sequence Comparison ◽  
Drosophila Hydei ◽  
Myosin Heavy Chain Genes ◽  
Muscle Myosin
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