Synergistic effects of stromal cell-derived factor-1α and bone morphogenetic protein-2 treatment on odontogenic differentiation of human stem cells from apical papilla cultured in the VitroGel 3D system

2019 ◽  
Vol 378 (2) ◽  
pp. 207-220 ◽  
Author(s):  
Min Xiao ◽  
Jun Qiu ◽  
Rong Kuang ◽  
Beidi Zhang ◽  
Wei Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Morphogenetic Protein ◽  
Stromal Cell ◽  
Synergistic Effects ◽  
Bone Morphogenetic Protein 2 ◽  
Human Stem Cells ◽  
Odontogenic Differentiation ◽  
Morphogenetic Protein ◽  
Apical Papilla ◽  
Stromal Cell Derived Factor

scholarly journals Foxc2 and BMP2 Induce Osteogenic/Odontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Wen Zhang ◽  
Xiaolei Zhang ◽  
Junyuan Li ◽  
Jianmao Zheng ◽  
Xiaoli Hu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Human Stem Cells ◽  
Alizarin Red ◽  
Time Points ◽  
Odontogenic Differentiation ◽  
Morphogenetic Protein ◽  
Apical Papilla

As a transcription factor regulated by bone morphogenetic protein 2 (BMP2), Forkhead c2 (Foxc2) plays a pivot role in osteogenesis/odontogenesis. However, the role of Foxc2 and BMP2 in regulating osteo-/odontogenic differentiation and mineralization of stem cells from apical papilla (SCAP) is still uncertain. In this research, overexpression of Foxc2 gene significantly improved the proliferation of SCAP four days and eight days after transfection, but overexpression of both Foxc2 and BMP2 genes significantly inhibited the proliferation of SCAP eight days after transfection. RT-qPCR and western blot results indicated that SCAP-Foxc2-BMP2 significantly upregulated osteo-/odontogenic genes and proteins at most of the time points in SCAP after transfection. Moreover, SCAP-Foxc2-BMP2 formed notably more alkaline phosphatase-positive and alizarin red-positive mineralized nodules than other three group cells sixteen days after transfection. In conclusion, our findings revealed that Foxc2 and BMP2 synergistically promoted osteo-/odontogenic differentiation and mineralization of SCAP in vitro.

Stromal Cell-Derived Factor-1 Potentiates Bone Morphogenetic Protein-2 Induced Bone Formation

2011 ◽  
Vol 17 (3-4) ◽  
pp. 523-530 ◽  
Author(s):  
Kosaku Higashino ◽  
Manjula Viggeswarapu ◽  
Maggie Bargouti ◽  
Hui Liu ◽  
Louisa Titus ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Stromal Cell ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Stromal Cell Derived Factor
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