Gastric smooth muscle cells manifest an abnormal phenotype in Parkinson’s disease rats with gastric dysmotility

2020 ◽  
Vol 381 (2) ◽  
pp. 217-227 ◽  
Author(s):  
Xiao-Lin Xiu ◽  
Li-Fei Zheng ◽  
Xiao-Yu Liu ◽  
Yan-Yan Fan ◽  
Jin-Xia Zhu
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Abnormal Phenotype ◽  
Gastric Smooth Muscle

Characterization of opioid receptors on smooth muscle cells from guinea pig stomach

1992 ◽  
Vol 262 (3) ◽  
pp. G461-G469 ◽  
Author(s):  
L. Zhang ◽  
Z. F. Gu ◽  
T. Pradhan ◽  
R. T. Jensen ◽  
P. N. Maton
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Opioid Receptors ◽  
Muscle Cells ◽  
Receptor Subtypes ◽  
Selective Antagonist ◽  
Kappa Agonist ◽  
Gastric Smooth Muscle ◽  
Selective Ligand ◽  
Guinea Pig Stomach

On the basis of opioid-stimulated contraction of dispersed gastric smooth muscle cells it has been suggested that these cells possess opioid receptors of three subtypes: kappa (kappa), mu (mu), and delta (delta). We have used selective peptidase-resistant radioligands, agonists and antagonists, to examine receptor subtypes on dispersed gastric smooth muscle cells from guinea pigs prepared by collagenase digestion. The kappa-agonist U-50488H, the mu-agonist [D-Ala2,N-Me-Phe4,Gly5-ol]enkephalin (DAGO), and the delta-agonist [D-Pen2,Pen5]enkephalin (DPDPE) each caused muscle contraction. The concentrations required to caused half-maximal contraction were U50488H (6 pM) greater than DAGO (13 pM) greater than DPDPE (6 nM). The abilities of these agonists to inhibit binding of [3H]U-69593 (kappa-preferring) by 50% were U50488H (43 nM) greater than DAGO (43 microM) greater than DPDPE (200 microM). Their abilities to inhibit binding of [3H]naloxone (mu-preferring) by 50% were DAGO (0.2 microM) greater than U50488H (10 microM) greater than DPDPE (greater than 100 microM). No binding could be detected with the delta-selective ligand [3H]DPDPE. The kappa-preferring antagonist Mr2266 (10 nM) preferentially inhibited contraction stimulated by the kappa-agonist U50488H, and naltrexone (10 nM) (mu-selective antagonist) preferentially inhibited contraction stimulated by the mu-agonist DAGO. ICI 174864 (200 microM; delta-selective antagonist) had no effect on contraction stimulated by mu-, kappa-, or delta-agonists. Contraction stimulated by the delta-agonist DPDPE was inhibited by both kappa- and mu-receptor antagonists. Studies on the effect of the antagonists on binding of [3H]naloxone and [3H]U69593 also provided evidence for kappa- and mu-sites but nor for delta-sites.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Non-genomic effects of progesterone on Rho kinase II in rat gastric smooth muscle cells

2013 ◽  
Vol 49 (0) ◽  
pp. 55-62 ◽  
Author(s):  
Othman Al-Shboul ◽  
Ayman Mustafa ◽  
Farah Al-hashimi
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Rho Kinase ◽  
Muscle Cells ◽  
Gastric Smooth Muscle ◽  
Genomic Effects

Role of Potassium Channels in the Effects of Hydrogen Sulfide on Contractility of Gastric Smooth Muscle Cells in Rats

2018 ◽  
Vol 54 (5) ◽  
pp. 400-407 ◽  
Author(s):  
I. F. Shaidullov ◽  
M. U. Shafigullin ◽  
L. M. Gabitova ◽  
F. G. Sitdikov ◽  
A. L. Zefirov ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Hydrogen Sulfide ◽  
Potassium Channels ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Gastric Smooth Muscle

Muscarinic receptor size on smooth muscle cells and membranes

1986 ◽  
Vol 251 (2) ◽  
pp. G195-G200
Author(s):  
S. M. Collins ◽  
C. Y. Jung ◽  
A. K. Grover
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscarinic Receptor ◽  
Plasma Membranes ◽  
Muscle Cells ◽  
High Energy ◽  
Membrane Preparation ◽  
Galactose Oxidase ◽  
Radiation Inactivation ◽  
Gastric Smooth Muscle

The loss of [3H]quinuclidinyl benzilate ([3H]QNB) binding following high-energy radiation was used to compare the muscarinic receptor size on single smooth muscle cells isolated by collagenase digestion from the canine stomach and on plasma membranes derived from intact gastric smooth muscle without exposure to exogenous proteolysis. Radiation inactivation of galactose oxidase (68 kdaltons), yeast alcohol dehydrogenase (160 kdaltons), and pyruvate kinase (224 kdaltons) activities were used as molecular-weight standards. Radiation inactivation of [3H]QNB binding to rat brain membranes, which gave a target size of 86 kdaltons, served as an additional control. In isolated smooth muscle cells, the calculated size of the muscarinic receptor was 80 +/- 8 kdaltons. In contrast, in a smooth muscle enriched plasma membrane preparation, muscarinic receptor size was significantly smaller at 45 +/- 3 kdaltons. Larger molecular sizes were obtained either in the presence of protease inhibitors (62 +/- 4 kdaltons) or by using a crude membrane preparation of gastric smooth muscle 86 +/- 7 kdaltons).

Gastric smooth muscle cells possess two classes of endothelin receptors but only one alters contraction

1994 ◽  
Vol 266 (4) ◽  
pp. G713-G721 ◽  
Author(s):  
Y. Kitsukawa ◽  
Z. F. Gu ◽  
P. Hildebrand ◽  
R. T. Jensen
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Contraction ◽  
Muscle Cells ◽  
The Other ◽  
Stomach Muscle ◽  
Dependent Manner ◽  
High Affinity ◽  
Gastric Smooth Muscle ◽  
Eta Receptor

Endothelin (ET)-like immunoreactivity and ET binding sites are widely distributed in the gastrointestinal tract, and ET causes contraction of stomach muscle strips. To determine whether ETs could interact with gastric smooth muscle cells directly and alter function, we measured binding of 125I-ET-1, 125I-ET-2, and 125I-ET-3 to dispersed gastric smooth muscle cells from guinea pig and their abilities to alter cell length. Each ligand bound in a time- and temperature-dependent manner, which was specific and saturable. Analysis of the dose-inhibition curves of both ET-1 and ET-3 for binding of each ligand indicated the presence of two classes of receptors, one class (ETA receptor) with a high affinity for ET-1 and ET-2 but a low affinity for ET-3, and the other (ETB receptor) with a high affinity for ET-1, ET-2, and ET-3. The ligands were rapidly internalized by both receptors; however, it was greater with ETA receptors. ET-1 stimulated muscle contraction (50% effective concentration approximately 2 nM), whereas ET-3 did not stimulate contraction or cause relaxation. These results demonstrate that gastric smooth muscle cells possess two classes of ET receptors. One type (ETA) has a high affinity for ET-1 and ET-2 and a low affinity for ET-3, and receptor occupation results in rapid ligand internalization and muscle contraction; the other type (ETB) has a high affinity for ET-1, ET-2, and ET-3, and receptor occupation results in a lesser degree of ligand internalization than the ETA receptor and does not alter contractile behavior.

scholarly journals Erratum to: Role of Potassium Channels in the Effects of Hydrogen Sulfide on Contractility of Gastric Smooth Muscle Cells in Rats

2019 ◽  
Vol 55 (1) ◽  
pp. 84-84
Author(s):  
I. F. Shaidullov ◽  
M. U. Shafigullin ◽  
L. M. Gabitova ◽  
F. G. Sitdikov ◽  
A. L. Zefirov ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Hydrogen Sulfide ◽  
Potassium Channels ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Gastric Smooth Muscle

Smooth muscle cells from guinea pig stomach possess high-affinity galanin receptors that mediate relaxation

1994 ◽  
Vol 266 (5) ◽  
pp. G839-G845 ◽  
Author(s):  
Z. F. Gu ◽  
T. K. Pradhan ◽  
D. H. Coy ◽  
R. T. Jensen
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Maximal Effect ◽  
Dependent Manner ◽  
High Affinity ◽  
Gastric Smooth Muscle ◽  
Guinea Pig Stomach ◽  
Galanin Receptors

Galanin-like immunoactivity occurs in nerves and plexi in muscle layers throughout gastrointestinal tract including the stomach. Galanin can affect gastric emptying and contraction or relaxation of gastric muscle in different species. The aim of this study was to investigate the direct effect of galanin on dispersed gastric smooth muscle cells and to characterize any galanin receptors that mediated any effect. Dispersed gastric smooth muscle cells were prepared from guinea pig stomach by collagenase digestion. Porcine galanin (p-galanin; 1 microM) did not stimulate contraction when present alone; however, p-galanin (1 microM) inhibited carbachol-induced contraction with a half-maximal effect at 7 nM. p-Galanin (1 microM) increased cellular adenosine 3',5'-cyclic monophosphate (cAMP) content by 10 s and caused a maximal increase of 80% over basal. 125I-galanin (porcine) bound to dispersed cells in a time- and temperature-dependent manner. Binding was saturable, reversible, and specific. Binding of 125I-galanin was inhibited almost equally by porcine and rat galanin (Ki = 6-8 nM) but was not inhibited by the galanin-associated peptide [preprogalanin-(108-123)]. The fragment galanin-(1-16) was equally potent to rat galanin; however, the fragment galanin-(9-29) was 56-fold less potent (Ki = 370 nM). Computer analysis demonstrated there were two binding sites for p-galanin on gastric smooth muscle cells, a high-affinity site (Kd = 2.6 nM) with low capacity (Bmax = 175 fmol/mg protein) and a low-affinity site (Kd = 150 nM) with large capacity (Bmax = 3,611 fmol/mg protein).(ABSTRACT TRUNCATED AT 250 WORDS)

Nonadrenergic inhibitory postsynaptic potentials of gastric smooth muscle cells

1973 ◽  
Vol 4 (2) ◽  
pp. 165-169
Author(s):  
E. S. Atanasova ◽  
I. A. Vladimirova ◽  
M. F. Shuba
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Postsynaptic Potentials ◽  
Inhibitory Postsynaptic Potentials ◽  
Gastric Smooth Muscle
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