Enhanced amylase production by Bacillus subtilis using a dual exponential feeding strategy

2004 ◽  
Vol 27 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Hanjing Huang ◽  
Darin Ridgway ◽  
Tingyue Gu ◽  
Murray Moo-Young
Keyword(s):  
Bacillus Subtilis ◽  
Feeding Strategy ◽  
Exponential Feeding ◽  
Amylase Production

EFFECT OF L-CYSTEINE ON α-AMYLASE PRODUCTION AND NUCLEIC ACID METABOLISM IN BACILLUS SUBTILIS

1963 ◽  
Vol 9 (3) ◽  
pp. 337-341 ◽  
Author(s):  
MICHIO OISHI ◽  
SHIGERU KITAYAMA ◽  
HAJIME TAKAHASHI ◽  
BUNJI MARUO
Keyword(s):  
Bacillus Subtilis ◽  
Nucleic Acid ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism ◽  
Amylase Production

scholarly journals Modular pathway engineering of key precursor supply pathways for lacto-N-neotetraose production in Bacillus subtilis

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiaomin Dong ◽  
Nan Li ◽  
Zhenmin Liu ◽  
Xueqin Lv ◽  
Jianghua Li ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Human Milk ◽  
Feeding Strategy ◽  
Intestinal Bacteria ◽  
Fine Tuning ◽  
Pathway Engineering ◽  
Cell Maturation ◽  
Lactose Permease ◽  
Human Milk Oligosaccharides ◽  
Precursor Supply

Abstract Background Lacto-N-neotetraose (LNnT) is one of the important ingredients of human milk oligosaccharides, which can enhance immunity, regulate intestinal bacteria and promote cell maturation. Results In this study, the synthetic pathway of LNnT was constructed by co-expressing the lactose permease (LacY) β-1,3-N-acetylglucosaminyltransferase (LgtA) and β-1,4-galactostltransferase (LgtB) in Bacillus subtilis, resulting in an LNnT titer of 0.61 g/L. Then, by fine-tuning the expression level of LgtB, the growth inhibition was reduced and the LNnT titer was increased to 1.31 g/L. In addition, by modular pathway engineering, the positive-acting enzymes of the UDP-GlcNAc and UDP-Gal pathways were strengthened to balance the two key precursors supply, and the LNnT titer was improved to 1.95 g/L. Finally, the LNnT titer reached 4.52 g/L in a 3-L bioreactor with an optimal glucose and lactose feeding strategy. Conclusions In general, this study showed that the LNnT biosynthesis could be significantly increased by optimizing enzymes expression levels and modular pathway engineering for balancing the precursors supply in B. subtilis.

α-Amylase Production by Free and Immobilized Bacillus subtilis

2000 ◽  
Vol 84-86 (1-9) ◽  
pp. 479-486 ◽  
Author(s):  
Enrique Duran-Paramo ◽  
Oscar Garcia-Kirchner ◽  
Jean-François Hervagault ◽  
Daniel Thomas ◽  
Jean-Noël Barbotin
Keyword(s):  
Bacillus Subtilis ◽  
Amylase Production

scholarly journals Homogeneity and heterogeneity in amylase production by Bacillus subtilis under different growth conditions

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Tina N. Ploss ◽  
Ewoud Reilman ◽  
Carmine G. Monteferrante ◽  
Emma L. Denham ◽  
Sjouke Piersma ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Conditions ◽  
Amylase Production

A segregated model for heterologous amylase production by Bacillus subtilis

2003 ◽  
Vol 32 (3-4) ◽  
pp. 407-413 ◽  
Author(s):  
Hanjing Huang ◽  
Darin Ridgway ◽  
Tingyue Gu ◽  
Murray Moo-Young
Keyword(s):  
Bacillus Subtilis ◽  
Amylase Production ◽  
Segregated Model
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