Feeding strategies for the enhanced production of α-arbutin in the fed-batch fermentation of Xanthomonas maltophilia BT-112

2013 ◽  
Vol 37 (2) ◽  
pp. 325-329 ◽  
Author(s):  
Chunqiao Liu ◽  
Peng Zhang ◽  
Shurong Zhang ◽  
Tao Xu ◽  
Fang Wang ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Xanthomonas Maltophilia ◽  
Enhanced Production ◽  
Fed Batch Fermentation

scholarly journals DIFFERENT FRUCTOSE FEEDING STRATEGIES FOR POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Yangia sp. ND199

2017 ◽  
Vol 55 (2) ◽  
pp. 195
Author(s):  
Truong Huu Phong ◽  
Dang Minh Khuong ◽  
Duong Van Hop ◽  
Doan Van Thuoc
Keyword(s):  
Batch Fermentation ◽  
Halophilic Bacteria ◽  
Carbon Sources ◽  
Dry Weight ◽  
Halophilic Bacterium ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Mangrove Soil ◽  
Fed Batch Fermentation ◽  
Maximum Cell

Yangia sp. ND199 is a halophilic bacterium isolated from mangrove soil sample. This strain was able to produce polyhydroxyalkanoate (PHA) from different carbon sources. Only homopolymer poly(3-hydroxybutyrate) (PHB) was synthesized when fructose was used as carbon source. The bacterium can accumulate high PHB content during exponential phase. Maximum cell dry weight (CDW) of 7.8 g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fed-batch fermentation with different fructose feeding strategies. The highest CDW of 78.5 g/l, PHB content of 67.5 wt%, and PHB productivity of 1 g/l/h were obtained by using two-stage fed-batch fermentation, is among the highest reported so far for PHB production by halophilic bacteria.         

scholarly journals Production of L (+) Lactic Acid by Lactobacillus casei Ke11: Fed Batch Fermentation Strategies

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 151
Author(s):  
Paola Monteiro de Oliveira ◽  
Larissa Provasi Santos ◽  
Luciana Fontes Coelho ◽  
Paulo Marcelo Avila Neto ◽  
Daiane Cristina Sass ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Batch Fermentation ◽  
Lactobacillus Casei ◽  
Culture Media ◽  
Feeding Strategy ◽  
High Yield ◽  
Feeding Strategies ◽  
Efficient Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Lactic acid and its derivatives are widely used in pharmaceutical, leather, textile and food industries. However, until now there have been few systematic reports on fed-batch fermentation for efficient production and high concentration of l-lactic acid by lactic acid bacteria. This study describes the obtainment of L (+) lactic acid from sucrose using the Lactobacillus casei Ke11 strain through different feeding strategies using an accessible pH neutralizer such as CaCO3. The exponential feeding strategy can increase lactic acid production and productivity (175.84 g/L and 3.74 g/L/h, respectively) with a 95% yield, avoiding inhibition by high initial substrate concentration and, combined with the selected agent controller, avoids the cellular stress that could be caused by the high osmotic pressure of the culture media. The purification of the acid using charcoal and celite, followed by the use of a cation exchange column proved to be highly efficient, allowing a high yield of lactic acid, high removal of sugars and proteins. The described process shows great potential for the production of lactic acid, as well as the simple, efficient and low-cost purification method. This way, this work is useful to the large-scale fermentation of L. casei Ke11 for production of l-lactic acid.

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