scholarly journals DIFFERENT FRUCTOSE FEEDING STRATEGIES FOR POLY(3-HYDROXYBUTYRATE) PRODUCTION BY Yangia sp. ND199

2017 ◽  
Vol 55 (2) ◽  
pp. 195
Author(s):  
Truong Huu Phong ◽  
Dang Minh Khuong ◽  
Duong Van Hop ◽  
Doan Van Thuoc
Keyword(s):  
Batch Fermentation ◽  
Halophilic Bacteria ◽  
Carbon Sources ◽  
Dry Weight ◽  
Halophilic Bacterium ◽  
Feeding Strategies ◽  
Fed Batch ◽  
Mangrove Soil ◽  
Fed Batch Fermentation ◽  
Maximum Cell

Yangia sp. ND199 is a halophilic bacterium isolated from mangrove soil sample. This strain was able to produce polyhydroxyalkanoate (PHA) from different carbon sources. Only homopolymer poly(3-hydroxybutyrate) (PHB) was synthesized when fructose was used as carbon source. The bacterium can accumulate high PHB content during exponential phase. Maximum cell dry weight (CDW) of 7.8 g/l and PHB content of 49 wt% were obtained after 27 h of cultivation in batch fermentation. High CDW and PHB content were achieved by using fed-batch fermentation with different fructose feeding strategies. The highest CDW of 78.5 g/l, PHB content of 67.5 wt%, and PHB productivity of 1 g/l/h were obtained by using two-stage fed-batch fermentation, is among the highest reported so far for PHB production by halophilic bacteria.         

scholarly journals Spruce sugars and poultry hydrolysate as growth medium in repeated fed-batch fermentation processes for production of yeast biomass

2019 ◽  
Vol 43 (4) ◽  
pp. 723-736 ◽  
Author(s):  
David Lapeña ◽  
Pernille M. Olsen ◽  
Magnus Ø. Arntzen ◽  
Gergely Kosa ◽  
Volkmar Passoth ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Dry Weight ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Growth Media ◽  
Fish Feed ◽  
Fed Batch ◽  
Wickerhamomyces Anomalus ◽  
Fed Batch Fermentation ◽  
Lignocellulosic Sugars

AbstractThe production of microbial protein in the form of yeast grown on lignocellulosic sugars and nitrogen-rich industrial residues is an attractive approach for reducing dependency on animal and plant protein. Growth media composed of enzymatically saccharified sulfite-pulped spruce wood, enzymatic hydrolysates of poultry by-products and urea were used for the production of single-cell protein. Strains of three different yeast species, Cyberlindnera jadinii, Wickerhamomyces anomalus and Blastobotrys adeninivorans, were cultivated aerobically using repeated fed-batch fermentation up to 25 L scale. Wickerhamomyces anomalus was the most efficient yeast with yields of 0.6 g of cell dry weight and 0.3 g of protein per gram of glucose, with cell and protein productivities of 3.92 g/L/h and 1.87 g/L/h, respectively. Using the conditions developed here for producing W. anomalus, it would take 25 industrial (200 m3) continuously operated fermenters to replace 10% of the fish feed protein used in Norway.

scholarly journals High-Cell-Density Cyclic Fed-Batch Fermentation of a Poly(3-Hydroxybutyrate)-Accumulating Thermophile, Chelatococcus sp. Strain MW10

2010 ◽  
Vol 76 (23) ◽  
pp. 7890-7895 ◽  
Author(s):  
Mohammad H. A. Ibrahim ◽  
Alexander Steinb�chel
Keyword(s):  
Cell Density ◽  
Batch Fermentation ◽  
High Cell Density ◽  
Dry Weight ◽  
Thermophilic Bacterium ◽  
High Cell ◽  
Fed Batch ◽  
G Cell ◽  
Fed Batch Fermentation

ABSTRACT Different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium, Chelatococcus sp. strain MW10, with the aim of achieving high-cell-density (HCD) growth and high poly(3-hydroxybutyrate) [poly(3HB)] productivity. Enhanced cultivation was achieved by a cyclic fed-batch fermentation (CFBF) technique (42-liter scale). Maximal poly(3HB) productivity was obtained during the second cycle [16.8 � 4.2 g poly(3HB)/liter]. At the end of CFBF (265 h), an HCD of up to 115.0 � 4.3 g cell dry weight/liter was achieved.

scholarly journals Maximization of Siderophores Production from Biocontrol Agents, Pseudomonas aeruginosa F2 and Pseudomonas fluorescens JY3 Using Batch and Exponential Fed-Batch Fermentation

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 455 ◽  
Author(s):  
Gaber Attia Abo-Zaid ◽  
Nadia Abdel-Mohsen Soliman ◽  
Ahmed Salah Abdullah ◽  
Ebaa Ebrahim El-Sharouny ◽  
Saleh Mohamed Matar ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Dry Weight ◽  
Antagonistic Activity ◽  
Culture Broth ◽  
Biocontrol Agents ◽  
Bacterial Isolates ◽  
Damping Off ◽  
Fed Batch ◽  
Fluorescent Pseudomonas ◽  
Fed Batch Fermentation

Twenty fluorescent Pseudomonas isolates were tested for their ability to produce siderophores on chrome azurol S (CAS) agar plates and their antagonistic activity against six plant pathogenic fungal isolates was assessed. Scaling-up production of siderophores from the promising isolates, P. aeruginosa F2 and P. fluorescens JY3 was performed using batch and exponential fed-batch fermentation. Finally, culture broth of the investigated bacterial isolates was used for the preparation of two economical bioformulations for controlling Fusarium oxysporum and Rhizoctonia solani. The results showed that both isolates yielded high siderophore production and they were more effective in inhibiting the mycelial growth of the tested fungi compared to the other bacterial isolates. Exponential fed-batch fermentation gave higher siderophore concentrations (estimated in 10 µL), which reached 67.05% at 46 h and 45.59% at 48 h for isolates F2 and JY3, respectively, than batch fermentation. Formulated P. aeruginosa F2 and P. fluorescens JY3 decreased the damping-off percentage caused by F. oxysporum with the same percentage (80%), while, the reduction in damping-off percentage caused by R. solani reached 87.49% and 62.5% for F2 and JY3, respectively. Furthermore, both formulations increased the fresh and dry weight of shoots and roots of wheat plants. In conclusion, bio-friendly formulations of siderophore-producing fluorescent Pseudomonas isolates can be used as biocontrol agents for controlling some plant fungal diseases.

scholarly journals Production of L (+) Lactic Acid by Lactobacillus casei Ke11: Fed Batch Fermentation Strategies

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 151
Author(s):  
Paola Monteiro de Oliveira ◽  
Larissa Provasi Santos ◽  
Luciana Fontes Coelho ◽  
Paulo Marcelo Avila Neto ◽  
Daiane Cristina Sass ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Batch Fermentation ◽  
Lactobacillus Casei ◽  
Culture Media ◽  
Feeding Strategy ◽  
High Yield ◽  
Feeding Strategies ◽  
Efficient Production ◽  
Fed Batch ◽  
Fed Batch Fermentation

Lactic acid and its derivatives are widely used in pharmaceutical, leather, textile and food industries. However, until now there have been few systematic reports on fed-batch fermentation for efficient production and high concentration of l-lactic acid by lactic acid bacteria. This study describes the obtainment of L (+) lactic acid from sucrose using the Lactobacillus casei Ke11 strain through different feeding strategies using an accessible pH neutralizer such as CaCO3. The exponential feeding strategy can increase lactic acid production and productivity (175.84 g/L and 3.74 g/L/h, respectively) with a 95% yield, avoiding inhibition by high initial substrate concentration and, combined with the selected agent controller, avoids the cellular stress that could be caused by the high osmotic pressure of the culture media. The purification of the acid using charcoal and celite, followed by the use of a cation exchange column proved to be highly efficient, allowing a high yield of lactic acid, high removal of sugars and proteins. The described process shows great potential for the production of lactic acid, as well as the simple, efficient and low-cost purification method. This way, this work is useful to the large-scale fermentation of L. casei Ke11 for production of l-lactic acid.

scholarly journals Conversion of Glycerol to Poly(3-Hydroxypropionate) in Recombinant Escherichia coli

2009 ◽  
Vol 76 (2) ◽  
pp. 622-626 ◽  
Author(s):  
Björn Andreeßen ◽  
Alvin Brian Lange ◽  
Horst Robenek ◽  
Alexander Steinbüchel
Keyword(s):  
Escherichia Coli ◽  
Batch Fermentation ◽  
Ralstonia Eutropha ◽  
Dry Weight ◽  
Recombinant Escherichia Coli ◽  
Pha Synthase ◽  
Fed Batch ◽  
Interesting Application ◽  
Serovar Typhimurium ◽  
Fed Batch Fermentation

ABSTRACT We have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3HP)]. For this, the genes for glycerol dehydratase (dhaB1) of Clostridium butyricum, propionaldehyde dehydrogenase (pduP) of Salmonella enterica serovar Typhimurium LT2, and polyhydroxyalkanoate (PHA) synthase (phaC1) of Ralstonia eutropha were expressed in recombinant Escherichia coli. Poly(3HP) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. The present study shows an interesting application to engineer a poly(3HP) synthesis pathway in bacteria.

A Study of Fermentation for Human Interleukin-11 with Recombinant E. Coli

2005 ◽  
Vol 3 (1) ◽  
Author(s):  
Kaili Zhao ◽  
Yiru Gan ◽  
Zisheng Jason Zhang
Keyword(s):  
Carbon Source ◽  
Batch Fermentation ◽  
Carbon Sources ◽  
Biological Properties ◽  
Operational Parameters ◽  
Fed Batch ◽  
E Coli ◽  
Dry Cell Weight ◽  
Fed Batch Fermentation ◽  
Interleukin 11

Recombinant human interleukin-11(rhIL-11) is still the only therapy drug for thrombocytopenia. Although the biological properties and clinical behaviors of rhIL-11 have been studied extensively, its bioprocess development data have rarely been reported. In this work, fermentation conditions for recombinant E. coli, which expressed interleukin-11 as a fusion protein, were investigated. Batch and fed-batch fermentation experiments were performed in shake flasks and in a BioFlo fermenter with different medium and carbon sources under different operational parameters. Based on the results of the study, M9Ca medium was shown as the better medium and glycerol was identified to be a better carbon source than glucose for the expression of rhIL-11. When glycerol was used as the carbon source in fed-batch fermentation, a dry cell weight of 9.2 g/L and a rhIL-11 expression of 22%, were achieved. The results could be used as references in the optimization of the fermentation process.

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