In vitro transformation of adult rat hepatic progenitor cells into pancreatic endocrine hormone-producing cells

2008 ◽  
Vol 15 (3) ◽  
pp. 310-317 ◽  
Author(s):  
Hiroyuki Kawasaki ◽  
Toru Mizuguchi ◽  
Yamato Kikkawa ◽  
Hideki Oshima ◽  
Yasushi Sasaki ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Hepatic Progenitor Cells ◽  
Adult Rat ◽  
In Vitro Transformation ◽  
Endocrine Hormone

scholarly journals Identification of adult rat hepatic progenitor cells that can reconstitute the liver

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Mladen Yovchev ◽  
Petar Grozdanov ◽  
Hongchao Zhou ◽  
Chandan Guha ◽  
Mariana Dabeva
Keyword(s):  
Progenitor Cells ◽  
Hepatic Progenitor Cells ◽  
Adult Rat

scholarly journals Cooperation between PDGF and FGF converts slowly dividing O-2Aadult progenitor cells to rapidly dividing cells with characteristics of O-2Aperinatal progenitor cells.

1992 ◽  
Vol 118 (4) ◽  
pp. 889-900 ◽  
Author(s):  
G Wolswijk ◽  
M Noble
Keyword(s):  
Progenitor Cells ◽  
Adult Brain ◽  
High Rate ◽  
Adult Rat ◽  
Large Numbers ◽  
Dividing Cells ◽  
Rapid Generation ◽  
And Migration

We have shown previously that oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells isolated from adult rat optic nerves can be distinguished in vitro from their perinatal counterparts on the basis of their much slower rates of division, differentiation, and migration when grown in the presence of cortical astrocytes or PDGF. This behavior is consistent with in vivo observations that there is only a modest production of oligodendrocytes in the adult CNS. As such a behavior is inconsistent with the likely need for a rapid generation of oligodendrocytes following demyelinating damage to the mature CNS, we have been concerned with identifying in vitro conditions that allow O-2Aadult progenitor cells to generate rapidly large numbers of progeny cells. We now provide evidence that many slowly dividing O-2Aadult progenitor cells can be converted to rapidly dividing cells by exposing adult optic nerve cultures to both PDGF and bFGF. In addition, these O-2Aadult progenitor cells appear to acquire other properties of O-2Aperinatal progenitor cells, such as bipolar morphology and high rate of migration. Although many O-2Aadult progenitor cells in cultures exposed to bFGF alone also divide rapidly, these cells are multipolar and migrate little in vitro. Oligodendrocytic differentiation of O-2Aadult progenitor cells, which express receptors for bFGF in vitro, is almost completely inhibited in cultures exposed to bFGF or bFGF plus PDGF. As bFGF and PDGF appear to be upregulated and/or released after injury to the adult brain, this particular in vitro response of O-2Aadult progenitor cells to PDGF and bFGF may be of importance in the generation of large numbers of new oligodendrocytes in vivo following demyelination.

1082 MYOFIBROBLASTS MODULATE THE TRANS-DIFFERENTIATION OF HEPATIC PROGENITOR CELLS IN VITRO

2011 ◽  
Vol 54 ◽  
pp. S428-S429
Author(s):  
E. Fairhall ◽  
K. Wallace ◽  
K. Charlton ◽  
M. Wright
Keyword(s):  
Progenitor Cells ◽  
Hepatic Progenitor Cells

In Vitro Insulin Production and Analysis of Pancreatic Transcription Factors in Induced Human Hepatic Progenitor Cells

2010 ◽  
Vol 12 (5) ◽  
pp. 373-378 ◽  
Author(s):  
Aleem A. Khan ◽  
Alaganuru Rajendraprasad ◽  
N. Parveen ◽  
Mahaboob V. Shaik ◽  
Santosh K. Tiwari ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Progenitor Cells ◽  
Hepatic Progenitor Cells ◽  
Insulin Production

Neural Progenitor Cells Derived from the Adult Rat Subventricular Zone: Characterization and Transplantation

2007 ◽  
Vol 16 (8) ◽  
pp. 799-810 ◽  
Author(s):  
Kevin Chen ◽  
Stephanie M. Hughes ◽  
Bronwen Connor
Keyword(s):  
Progenitor Cells ◽  
Subventricular Zone ◽  
Neural Progenitor Cells ◽  
Therapeutic Potential ◽  
Neural Progenitor ◽  
Adult Rat ◽  
Cell Purification ◽  
Adult Rat Brain ◽  
Percoll Density Gradient

In order to fully characterize and determine the therapeutic potential of adult neural progenitor cells (NPCs), it is important to be able to isolate and study NPCs from animals such as rats, in which there are existing models of brain injury and disease. The focus of this study was to characterize the cultivation, differentiation, and transplantation of adult rat NPCs isolated from the subventricular zone of the lateral ventricles. We examined strategies for cell purification using a Percoll density gradient, and cell expansion using a range of maintenance medium and plating densities. Purification by Percoll gradient enriched a population of cells expressing nestin and SOX2, but resulted in a significant reduction in neurosphere generation. Culturing adult rat NPCs in Neurobasal-A media and plating at 200,000 cell/ml resulted in a higher percentage of cells surviving to generate neurospheres compared to culture in DMEM/F12 or NS-A media. On induction of differentiation, adult rat NPCs were capable of generating neurons, astrocytes, and oligodendrocytes in vitro that survived for up to 8 weeks, demonstrating multipotentiality of these cells. In addition, a population of cells continued to proliferate during the initial phase of differentiation, suggesting the presence of two populations of NPCs during differentiation. Cultured adult rat NPCs also survived and differentiated into astrocytes 6 weeks after transplantation into the striatum of the normal adult rat brain. In conclusion, we have optimized techniques that allow for the routine isolation, culture, and transplantation of multipotent NPCs derived from the adult rat SVZ.

scholarly journals Two populations of Thy1-positive mesenchymal cells regulate in vitro maturation of hepatic progenitor cells

2007 ◽  
Vol 292 (2) ◽  
pp. G526-G534 ◽  
Author(s):  
Naoko Kamo ◽  
Kentaro Yasuchika ◽  
Hideaki Fujii ◽  
Toshitaka Hoppo ◽  
Takafumi Machimoto ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
In Vitro Maturation ◽  
Periodic Acid ◽  
Mesenchymal Cell ◽  
Mesenchymal Cells ◽  
Pcr Analysis ◽  
Hepatic Progenitor Cells ◽  
Pas Staining ◽  
Two Populations

We previously reported that the in vitro maturation of CD49f+Thy1−CD45− (CD49f positive) fetal hepatic progenitor cells (HPCs) is supported by Thy1-positive mesenchymal cells derived from the fetal liver. These mesenchymal cell preparations contain two populations, one of a cuboidal shape and the other spindle shaped in morphology. In this study, we determined that the mucin-type transmembrane glycoprotein gp38 could distinguish cuboidal cells from spindle cells by immunocytochemistry. RT-PCR analysis revealed differences between isolated CD49f±Thy1+gp38+CD45− (gp38 positive) cells and CD49f±Thy1+gp38−CD45− (gp38 negative) cells, whereas both cells expressed mesenchymal cell markers. The coculture with gp38-positive cells promoted the maturation of CD49f-positive HPCs, which was estimated by positivity for periodic acid-Schiff (PAS) staining, whereas the coculture with gp38-negative cells maintained CD49f-positive HPCs negative for PAS staining. The expression of mature hepatocyte markers, such as tyrosine aminotransferase, tryptophan-2,3-dioxygenase, and glucose-6-phosphatase, were upregulated on HPCs by coculture with gp38-positive cells. Furthermore, transmission electron microscopy revealed the acquisition of mature hepatocyte features by HPCs cocultured with gp38-positive cells. This effect on maturation of HPCs was inhibited by the addition of conditioned medium derived from gp38-negative cells. By contrast, the upregulation of bromodeoxyuridine incorporation by HPCs demonstrated the proliferative effect of coculture with gp38-negative cells. In conclusion, these results suggest that in vitro maturation of HPCs promoted by gp38-positive cells may be opposed by an inhibitory effect of gp38-negative cells, which likely maintain the immature, proliferative state of HPCs.

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