Nucleotide sequence and infectious transcripts from a full-length cDNA clone of the carmovirus Melon necrotic spot virus

2003 ◽  
Vol 148 (3) ◽  
pp. 599-607 ◽  
Author(s):  
J. A. D�az ◽  
J. J. Bernal ◽  
E. Moriones ◽  
M. A. Aranda
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Full Length ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Full Length Cdna

scholarly journals Nucleotide sequence of a full-length cDNA clone encoding preproparathyroid hormone from pig and rat

1987 ◽  
Vol 15 (16) ◽  
pp. 6740-6740 ◽  
Author(s):  
Hans-Jürgen Schmelzer ◽  
Gerhard Gross ◽  
Georg Widera ◽  
Hubert Mayer
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Full Length ◽  
Full Length Cdna

scholarly journals Complete Nucleotide Sequence and Full-Length cDNA Clone of S.A.AR86, a South African Alphavirus Related to Sindbis

Virology ◽  
1996 ◽  
Vol 222 (2) ◽  
pp. 464-469 ◽  
Author(s):  
Dennis A. Simpson ◽  
Nancy L. Davis ◽  
Seh-Ching Lin ◽  
Darcy Russell ◽  
Robert E. Johnston
Keyword(s):  
Nucleotide Sequence ◽  
South African ◽  
Cdna Clone ◽  
Complete Nucleotide Sequence ◽  
Full Length ◽  
Full Length Cdna

scholarly journals Nucleotide Sequence and Infectivity of a Full-Length cDNA Clone of Panicum Mosaic Virus

Virology ◽  
1998 ◽  
Vol 241 (1) ◽  
pp. 141-155 ◽  
Author(s):  
Massimo Turina ◽  
Miko Maruoka ◽  
Judit Monis ◽  
A.O. Jackson ◽  
Karen-Beth G. Scholthof
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Cdna Clone ◽  
Full Length ◽  
Full Length Cdna

Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene family

1987 ◽  
Vol 9 (3) ◽  
pp. 197-203 ◽  
Author(s):  
Mark L. Tucker ◽  
Mary L. Durbin ◽  
Michael T. Clegg ◽  
Lowell N. Lewis
Keyword(s):  
Nucleotide Sequence ◽  
Gene Family ◽  
Cdna Clone ◽  
Full Length ◽  
Full Length Cdna

scholarly journals Nucleotide sequence of a full length cDNA clone of light harvesting chlorophyll a/b binding protein gene from green dark-grown pine (Pinus tunbergii) seedling

1988 ◽  
Vol 16 (24) ◽  
pp. 11829-11829 ◽  
Author(s):  
Naoki Yamamoto ◽  
Makoto Matsuoka ◽  
Yuriko Kano-Murakami ◽  
Yoshiyuki Tanaka ◽  
Ohashi Yuko
Keyword(s):  
Nucleotide Sequence ◽  
Chlorophyll A ◽  
Cdna Clone ◽  
Protein Gene ◽  
Light Harvesting ◽  
Binding Protein ◽  
Full Length ◽  
Full Length Cdna ◽  
Binding Protein Gene

scholarly journals Human delta-aminolevulinate dehydratase: nucleotide sequence of a full-length cDNA clone.

1986 ◽  
Vol 83 (20) ◽  
pp. 7703-7707 ◽  
Author(s):  
J. G. Wetmur ◽  
D. F. Bishop ◽  
C. Cantelmo ◽  
R. J. Desnick
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Full Length ◽  
Full Length Cdna ◽  
Aminolevulinate Dehydratase

scholarly journals Molecular Characterization of a Melon necrotic spot virus Strain That Overcomes the Resistance in Melon and Nonhost Plants

2004 ◽  
Vol 17 (6) ◽  
pp. 668-675 ◽  
Author(s):  
Juan A. Díaz ◽  
Cristina Nieto ◽  
Enrique Moriones ◽  
Verónica Truniger ◽  
Miguel A. Aranda
Keyword(s):  
Cdna Clone ◽  
Recessive Gene ◽  
Genomic Region ◽  
Open Reading Frames ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Melon Necrotic Spot Virus ◽  
Gomphrena Globosa ◽  
Resistance Trait ◽  
Cucumis Melo L

Resistance of melon (Cucumis melo L.) to Melon necrotic spot virus (MNSV) is inherited as a single recessive gene, denoted nsv. No MNSV isolates described to date (e.g., MNSV-Mα5), except for the MNSV-264 strain described here, are able to overcome the resistance conferred by nsv. Analysis of protoplasts of susceptible (Nsv/-) and resistant (nsv/nsv) melon cultivars inoculated with MNSV-264 or MNSV-Mα5 indicated that the resistance trait conferred by this gene is expressed at the single-cell level. The nucleotide sequence of the MNSV-264 genome has a high nucleotide identity with the sequences of other MNSV isolates, with the exception of its genomic 3′-untranslated region (3′-UTR), where less than 50% of the nucleotides are shared between MNSV-264 and the other two MNSV isolates completely sequenced to date. Uncapped RNAs transcribed from a full-length MNSV-264 cDNA clone were infectious and caused symptoms indistinguishable from those caused by the parental viral RNA. This cDNA clone allowed generation of chimeric mutants between MNSV-264 and MNSV-Mα5 through the exchange of the last 74 nucleotides of their coat protein (CP) open reading frames and the complete 3′-UTRs. Analysis of protoplasts of susceptible and resistant melon cultivars inoculated with chimeric mutants clearly showed that the MNSV avirulence determinant resides in the exchanged region. The carboxy-termini of the CP of both isolates are identical; therefore, the avirulence determinant likely consists of the RNA sequence itself. We also demonstrated that this genomic region contains the determinant for the unique ability of the isolate MNSV-264 to infect noncucurbit hosts (Nicotiana benthamiana and Gomphrena globosa).

Sign in / Sign up

Export Citation Format

Share Document