Nucleotide sequence and genomic organization of melon necrotic spot virus

Abstract A new distribution map is given for Melon necrotic spot virus (Tombusviridae: Carmovirus). The hosts include melon (Cucumis melo), cucumber (Cucumis sativus) and watermelon (Citrullus lanatus). Information is given on the geographical distribution in Europe (France, Greece, Crete, Italy, Sardinia, Netherlands, Norway, Spain, Canary islands, mainland Spain, Sweden, UK, England and Wales), Asia (China, Jiangsu, Iran, Israel, Japan, Hokkaido, Honshu, Kyushu, Shikoku, Korea Republic, Syria and Turkey), Africa (Tunisia), North America (Canada, Ontario, Mexico, USA, California) Central America and Caribbean (Guatemala, Honduras and Panama) and South America (Uruguay). The virus is transmitted by the fungal vector Olpidium bornovanus (syn. O. radicale) (Chytridiomycota: Olpidiaceae).

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The structure of melon necrotic spot virus determined at 2.8 Å resolution

Acta Crystallographica Section F Structural Biology and Crystallization Communications ◽

10.1107/s1744309107066481 ◽

2007 ◽

Vol 64 (1) ◽

pp. 8-13 ◽

Cited By ~ 11

Author(s):

Yasunobu Wada ◽

Hideaki Tanaka ◽

Eiki Yamashita ◽

Chikako Kubo ◽

Tamaki Ichiki-Uehara ◽

...

Keyword(s):

Necrotic Spot ◽

Spot Virus ◽

Melon Necrotic Spot Virus

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A new cryptic virus belonging to the family Partitiviridae was found in watermelon co-infected with Melon necrotic spot virus

Virus Genes ◽

10.1007/s11262-013-0937-8 ◽

2013 ◽

Vol 47 (2) ◽

pp. 382-384 ◽

Cited By ~ 10

Author(s):

Noa Sela ◽

Oded Lachman ◽

Victoria Reingold ◽

Aviv Dombrovsky

Keyword(s):

Necrotic Spot ◽

Spot Virus ◽

Cryptic Virus ◽

Melon Necrotic Spot Virus ◽

The Family

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Efficacy of composting infected plant residues in reducing the viability of Pepper mild mottle virus, Melon necrotic spot virus and its vector, the soil-borne fungus Olpidium bornovanus

Crop Protection ◽

10.1016/j.cropro.2009.12.021 ◽

2010 ◽

Vol 29 (4) ◽

pp. 342-348 ◽

Cited By ~ 4

Author(s):

M.I. Aguilar ◽

M.L. Guirado ◽

J.M. Melero-Vara ◽

J. Gómez

Keyword(s):

Infected Plant ◽

Mottle Virus ◽

Plant Residues ◽

Necrotic Spot ◽

Spot Virus ◽

Pepper Mild Mottle Virus ◽

Melon Necrotic Spot Virus ◽

Olpidium Bornovanus

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The Intra- and Intercellular Movement of Melon necrotic spot virus (MNSV) Depends on an Active Secretory Pathway

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-23-3-0263 ◽

2010 ◽

Vol 23 (3) ◽

pp. 263-272 ◽

Cited By ~ 44

Author(s):

Ainhoa Genovés ◽

Jose Antonio Navarro ◽

Vicente Pallás

Keyword(s):

Intracellular Transport ◽

Secretory Pathway ◽

Fluorescent Proteins ◽

Plant Viruses ◽

Cell Periphery ◽

Dependent Manner ◽

Necrotic Spot ◽

Spot Virus ◽

Melon Necrotic Spot Virus ◽

Viral Movement Protein

Plant viruses hijack endogenous host transport machinery to aid their intracellular spread. Here, we study the localization of the p7B, the membrane-associated viral movement protein (MP) of the Melon necrotic spot virus (MNSV), and also the potential involvement of the secretory pathway on the p7B targeting and intra- and intercellular virus movements. p7B fused to fluorescent proteins was located throughout the endoplasmic reticulum (ER) at motile Golgi apparatus (GA) stacks that actively tracked the actin microfilaments, and at the plasmodesmata (PD). Hence, the secretory pathway inhibitor, Brefeldin A (BFA), and the overexpression of the GTPase-defective mutant of Sar1p, Sar1[H74L], fully retained the p7B within the ER, revealing that the protein is delivered to PD in a BFA-sensitive and COPII-dependent manner. Disruption of the actin cytoskeleton with latrunculin B led to the accumulation of p7B in the ER, which strongly suggests that p7B is also targeted to the cell periphery in an actin-dependent manner. Remarkably, the local spread of the viral infection was significantly restricted either with the presence of BFA or under the overexpression of Sar1[H74L], thus revealing the involvement of an active secretory pathway in the intracellular movement of MNSV. Overall, these findings support a novel route for the intracellular transport of a plant virus led by the GA.

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