Genomic characterization of pea enation mosaic virus-2 from the Pacific Northwestern USA

Begomoviruses are plant viruses that cause major losses to many economically important crops. Although they are poorly understood, begomoviruses infecting wild plants may have an important role as reservoirs in the epidemiology of viral diseases. This study reports the discovery and genomic characterization of three novel bipartite begomoviruses from wild and cultivated African basil (Ocimum gratissimum) plants collected in Uganda, East Africa. Based on the symptoms shown by the infected plants, the names proposed for these viruses are Ocimum yellow vein virus (OcYVV), Ocimum mosaic virus (OcMV), and Ocimum golden mosaic virus (OcGMV). Genome and phylogenetic analyses suggest that DNA-A of OcGMV is mostly related to begomoviruses infecting tomato in Africa, whereas those of OcYVV and OcMV are closely related to one another and highly divergent within the Old World begomoviruses. The DNA-A of all characterized begomovirus isolates are of a recombinant nature, revealing the role of recombination in the evolution of these begomoviruses. The viruses characterized here are the first identified in O. gratissimum and the first in Ocimum spp. in the African continent and could have important epidemiological consequences for cultivated basils and other important crops. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

Download Full-text

Genomic Characterization of a New Enamovirus Infecting Common Bean

10.21203/rs.3.rs-1038451/v1 ◽

2021 ◽

Author(s):

Ruo-bin Lu ◽

Ping-xiu Lan ◽

Ru-jing Kang ◽

Guan-lin Tan ◽

Xiao-jiao Chen ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Novel Species ◽

Open Reading Frames ◽

Amino Acid Sequence Level ◽

Disease Symptoms ◽

Bean Plants ◽

Genomic Characterization ◽

Pea Enation Mosaic Virus ◽

Reading Frames

Abstract A novel enamovirus was identified from bean plants with disease symptoms. Its genome of 5,781 nucleotides (nt) encodes five open reading frames. The virus and other species of the genus Enamovirus share identities of 50.4%-68.4% at the complete genome, and 19.9%-51.9% of P0, 24.9%-52.5% of P1, 33.4%-62.9% of P1-P2, 30.6%-81.1% of P3, 32.3%-74.2% of P3-P5 at amino acid sequence level, respectively. Phylogenetic analysis showed that the virus is most closely related to Alfalfa enamovirus 1 and Pea enation mosaic virus 1 in the genus Enamovirus within family Solemoviridae. These results suggest that the virus should be considered as a novel species in the genus Enamovirus and tentatively named as “bean enamovirus 1”.

Download Full-text

Characterization of a Satellite RNA Associated with Pea Enation Mosaic Virus

Journal of General Virology ◽

10.1099/0022-1317-70-5-1075 ◽

1989 ◽

Vol 70 (5) ◽

pp. 1075-1084 ◽

Cited By ~ 20

Author(s):

S. A. Demler ◽

G. A. De Zoeten

Keyword(s):

Mosaic Virus ◽

Satellite Rna ◽

Pea Enation Mosaic Virus

Download Full-text

Molecular and phenotypic characterization of variation related to pea enation mosaic virus resistance in lentil (Lens culinaris Medik.)

Canadian Journal of Plant Science ◽

10.4141/cjps-2014-077 ◽

2014 ◽

Vol 94 (8) ◽

pp. 1333-1344 ◽

Cited By ~ 1

Author(s):

Shalu Jain ◽

Lyndon D. Porter ◽

Ajay Kumar ◽

Reyazul R. Mir ◽

Sanford D. Eigenbrode ◽

...

Keyword(s):

Mosaic Virus ◽

Virus Resistance ◽

Lens Culinaris ◽

Polymorphic Information Content ◽

Field Tests ◽

Phenotypic Characterization ◽

Group Method ◽

Improvement Programs ◽

Pea Enation Mosaic Virus

Jain, S., Porter, L. D., Kumar, A., Mir, R. R., Eigenbrode, S. D. and McPhee, K. E. 2014. Molecular and phenotypic characterization of variation related to pea enation mosaic virus resistance in lentil (Lens culinaris Medik.). Can. J. Plant Sci. 94: 1333–1344. Identification of genetically diverse lentil germplasm with resistance to pea enation mosaic virus (PEMV) through the combined approach of molecular marker analysis and phenotyping could prove useful in breeding programs. A total of 44 lentil (Lens culinaris Medik.) accessions, were screened for resistance to PEMV. Two accessions (PI 431663 and PI 432028) were identified with resistance to PEMV in field tests while several accessions were found resistant in greenhouse screenings. Thirty-six polymorphic simple sequence repeat (SSR) markers which produced 43 loci with 2 to 12 alleles per locus were used for genetic diversity analysis. The polymorphic information content (PIC) values for these markers ranged from 0.22–0.85 with a mean of 0.55 per marker. Using allelic data of 36 SSR primer pairs, dissimilarity ranging from 0.12 to 0.74 was calculated. Cluster analysis performed using the unweighted pair group method with arithmetic mean (UPGMA) determined that most of PEMV-resistant accessions were grouped in one cluster along with other accessions from Iran, Chile, Ethiopia, India, Pakistan, Turkey, Afghanistan and Lebanon. All the adapted cultivars originating from North and South America were grouped in another cluster along with some European accessions. The 44 accessions were classified into 4 subpopulations using Structure 2.2 software complimenting the results of UPGMA analysis and indicated the effect of geographical origin on the grouping of accessions. The results of this study can be used to select genetically diverse PEMV-resistant accessions for lentil improvement programs.

Download Full-text

Alfalfa, a non-host of pea enation mosaic virus in Washington State

Canadian Journal of Plant Science ◽

10.4141/cjps96-095 ◽

1996 ◽

Vol 76 (3) ◽

pp. 521-524 ◽

Cited By ~ 9

Author(s):

Richard C. Larsen ◽

Robert E. Klein ◽

Walter J. Kaiser

Keyword(s):

Mosaic Virus ◽

Pacific Northwest ◽

Reservoir Host ◽

Washington State ◽

Enzyme Linked Immunosorbent Assay ◽

Dot Blot ◽

Coat Protein Region ◽

The Pacific ◽

Faba Beans ◽

Pea Enation Mosaic Virus

Extensive surveys were conducted in Washington State during a 6-yr period between 1988 and 1994 in an attempt to locate reservoirs of pea enation mosaic virus (PEMV). Many of the peas (Pisum sativum L.), chickpeas (Cicer arietinum L.), lentils (Lens culinaris Medic), or faba beans (Vicia faba L.) collected during the survey, and which expressed symptoms of virus infection, tested positive for PEMV when assayed by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). PEMV could not, however, be detected by ELISA in any of 3230 alfalfa samples collected from eight counties in Washington State, even when samples were taken from alfalfa fields adjacent to infected pea, lentil, or chickpea fields where the virus was present. Dot blot hybridizations using a 4556 nucleotide cDNA fragment of PEMV RNA 1 containing the coat protein region produced strong reactions with positive controls, and did not detect the virus in 597 alfalfa samples tested in 1994. Fifty-six attempts to transmit PEMV into and out of alfalfa by aphid and mechanical transmissions were unsuccessful, while successful transmissions to and from pea and faba bean, or chickpea were made routinely. Our inability to detect PEMV in, or transmit the virus to alfalfa suggests that alfalfa is not currently a host. The overwintering epidemiologically significant reservoir host(s) of PEMV in the Pacific Northwest remains unknown. Key words:Medicago sativa L., legumes, virus survey, pea enation mosaic virus

Download Full-text