Alfalfa, a non-host of pea enation mosaic virus in Washington State

1996 ◽  
Vol 76 (3) ◽  
pp. 521-524 ◽  
Author(s):  
Richard C. Larsen ◽  
Robert E. Klein ◽  
Walter J. Kaiser
Keyword(s):  
Mosaic Virus ◽  
Pacific Northwest ◽  
Reservoir Host ◽  
Washington State ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dot Blot ◽  
Coat Protein Region ◽  
The Pacific ◽  
Faba Beans ◽  
Pea Enation Mosaic Virus

Extensive surveys were conducted in Washington State during a 6-yr period between 1988 and 1994 in an attempt to locate reservoirs of pea enation mosaic virus (PEMV). Many of the peas (Pisum sativum L.), chickpeas (Cicer arietinum L.), lentils (Lens culinaris Medic), or faba beans (Vicia faba L.) collected during the survey, and which expressed symptoms of virus infection, tested positive for PEMV when assayed by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). PEMV could not, however, be detected by ELISA in any of 3230 alfalfa samples collected from eight counties in Washington State, even when samples were taken from alfalfa fields adjacent to infected pea, lentil, or chickpea fields where the virus was present. Dot blot hybridizations using a 4556 nucleotide cDNA fragment of PEMV RNA 1 containing the coat protein region produced strong reactions with positive controls, and did not detect the virus in 597 alfalfa samples tested in 1994. Fifty-six attempts to transmit PEMV into and out of alfalfa by aphid and mechanical transmissions were unsuccessful, while successful transmissions to and from pea and faba bean, or chickpea were made routinely. Our inability to detect PEMV in, or transmit the virus to alfalfa suggests that alfalfa is not currently a host. The overwintering epidemiologically significant reservoir host(s) of PEMV in the Pacific Northwest remains unknown. Key words:Medicago sativa L., legumes, virus survey, pea enation mosaic virus

Molecular characterization of pea enation mosaic virus and bean leafroll virus from the Pacific Northwest, USA

2010 ◽  
Vol 155 (10) ◽  
pp. 1713-1715 ◽  
Author(s):  
B. Vemulapati ◽  
K. L. Druffel ◽  
S. D. Eigenbrode ◽  
A. Karasev ◽  
H. R. Pappu
Keyword(s):  
Mosaic Virus ◽  
Molecular Characterization ◽  
Pacific Northwest ◽  
The Pacific ◽  
Bean Leafroll Virus ◽  
Leafroll Virus ◽  
Pea Enation Mosaic Virus

scholarly journals Identification of a Kulshan caldera correlative tephra in the Palouse loess of Washington State, northwest USA

2016 ◽  
Vol 86 (2) ◽  
pp. 232-241 ◽  
Author(s):  
Georgina E. King ◽  
Nicholas J.G. Pearce ◽  
Helen M. Roberts ◽  
Victoria C. Smith ◽  
John A. Westgate ◽  
...  
Keyword(s):  
Trace Element ◽  
Pacific Northwest ◽  
Washington State ◽  
Fractional Crystallisation ◽  
Early Pleistocene ◽  
East Central ◽  
Trace Element Chemistry ◽  
The Pacific ◽  
Eruptive Volume ◽  
Reversed Polarity

AbstractThe Kulshan caldera formed at ∼1.15 Ma on the present-day site of Mt. Baker, Washington State, northwest USA and erupted a compositionally zoned (dacite-rhyolite) magma and a correlative eruptive, the Lake Tapps tephra. This tephra has previously been described, but only from the Puget Lowland of NW Washington. Here an occurrence of a Kulshan caldera correlative tephra is described from the Quaternary Palouse loess at the Washtucna site (WA-3). Site WA-3 is located in east-central Washington, ∼340 km southeast of the Kulshan caldera and ∼300 km east-southeast of the Lake Tapps occurrence in the Puget Lowland. Major- and trace element chemistry and location of the deposit at Washtucna within reversed polarity sediments indicates that it is not correlative with the Mesa Falls, Rockland, Bishop Ash, Lava Creek B or Huckleberry Ridge tephras. Instead the Washtucna deposit is related to the Lake Tapps tephra by fractional crystallisation, but is chemically distinct, a consequence of its eruption from a compositionally zoned magma chamber. The correlation of the Washtucna occurrence to the Kulshan caldera-forming eruption indicates that it had an eruptive volume exceeding 100 km3, and that its tephra could provide a valuable early-Pleistocene chronostratigraphic marker in the Pacific Northwest.

scholarly journals Effects of projected climate change on energy supply and demand in the Pacific Northwest and Washington State

2010 ◽  
Vol 102 (1-2) ◽  
pp. 103-128 ◽  
Author(s):  
Alan F. Hamlet ◽  
Se-Yeun Lee ◽  
Kristian E. B. Mickelson ◽  
Marketa M. Elsner
Keyword(s):  
Climate Change ◽  
Pacific Northwest ◽  
Energy Supply ◽  
Supply And Demand ◽  
Washington State ◽  
The Pacific

First Report of Downy Mildew of Chives Caused by Peronospora destructor in the Pacific Northwest

2003 ◽  
Vol 4 (1) ◽  
pp. 38
Author(s):  
Dean A. Glawe
Keyword(s):  
Downy Mildew ◽  
Pacific Northwest ◽  
Puget Sound ◽  
Washington State ◽  
King County ◽  
Specialty Crops ◽  
First Report ◽  
Peronospora Destructor ◽  
The Pacific ◽  
Allium Schoenoprasum

Chive (Allium schoenoprasum L.) is one of the specialty crops grown by farmers in the Puget Sound region of Washington State. In September, 2002, downy mildew symptoms were observed in a 0.2 hectare field planting of chive near Fall City, King County, WA. Downy mildew had not been reported previously on chive in the Pacific Northwest. Accepted for publication 15 April 2003. Published 12 May 2003.

Genomic characterization of pea enation mosaic virus-2 from the Pacific Northwestern USA

2011 ◽  
Vol 156 (10) ◽  
pp. 1897-1900 ◽  
Author(s):  
B. Vemulapati ◽  
K. L. Druffel ◽  
S. D. Eigenbrode ◽  
A. Karasev ◽  
H. R. Pappu
Keyword(s):  
Mosaic Virus ◽  
The Pacific ◽  
Genomic Characterization ◽  
Pea Enation Mosaic Virus

scholarly journals First Report of Cucumber Mosaic Virus in Eryngium amethystinum, Canna spp., and Aquilegia hybrids in Ohio

Plant Disease ◽  
1997 ◽  
Vol 81 (11) ◽  
pp. 1331-1331 ◽  
Author(s):  
J. R. Fisher ◽  
M.-C. Sanchez-Cuevas ◽  
S. T. Nameth ◽  
V. L. Woods ◽  
C. W. Ellett
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Plant Viruses ◽  
Early Summer ◽  
Reservoir Host ◽  
Enzyme Linked Immunosorbent Assay ◽  
Herbaceous Plant ◽  
First Report ◽  
Severe Stunting ◽  
Banding Profile

Eryngium amethystinum (amethyst sea holly) is a herbaceous plant commonly grown as an ornamental perennial in U.S.D.A. hardiness zones 3 to 8. The plant thrives in dry areas with infertile soils and the flowers are often used in dried floral arrangements. Canna spp. (Canna), soft perennials (U.S.D.A. zone 9 and above), are becoming popular flowering plants because of their bright flowers and spectacular foliage. There are a variety of species that fall under the heading Canna spp., of which the most popular are C. glauca, C. indica, C. edulis, and C. iridiflora. Hybrids of Aquilegia (garden columbine), a hardy perennial (U.S.D.A. zones 3 to 9), flower in late spring through early summer. The genus is made up of a wide variety of cultivars. E. amethystinum exhibiting severe mosaic, yellowing, and stunting, along with Canna plants exhibiting severe stunting, chlorotic and distorted foliage, and mosaic, and garden columbine plants exhibiting stunting, leaf curl, chlorosis, and mosaic, collected from commercial plantings throughout the central Ohio area, were analyzed for the presence of virus infection with viral-associated, double-stranded RNA (dsRNA) analysis. dsRNA analysis resulted in a banding profile typical of that seen with members of the cucumovirus family of plant viruses. Plants positive for cucumovurus-like dsRNA were tested with a direct antibody sandwich enzyme-linked immunosorbent assay (ELISA). ELISA results confirmed the presence of cucumber mosaic virus (CMV) in all symptomatic plants tested. No evidence of dsRNA or CMV was found in any of the asymptomatic plants tested. Because all of these hosts are common in the perennial garden, they could serve as a reservoir host of CMV for other plants in the garden. This is the first report of CMV in E. amethystinum, Canna spp., and Aquilegia hybrids in Ohio.

scholarly journals First Report of Alfalfa mosaic virus in Lavandula officinalis

Plant Disease ◽  
2004 ◽  
Vol 88 (8) ◽  
pp. 908-908 ◽  
Author(s):  
Ll. Martínez-Priego ◽  
M. C. Córdoba ◽  
C. Jordá
Keyword(s):  
Mosaic Virus ◽  
Screening Program ◽  
Sequence Data ◽  
Alfalfa Mosaic Virus ◽  
Mediterranean Area ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Coat Protein Region ◽  
C 30 ◽  
Lavandula Officinalis

For several years, in ornamental nurseries in the Mediterranean area of Spain, stunting and yellow leaf spotting have been observed in young plants of Lavandula officinalis. Symptoms eventually disappeared as the plants matured. During the summer of 2003, the number of plantlets affected and the intensity of symptoms increased significantly. Symptomatic plants tested positive using enzyme-linked immunosorbent assay (ELISA) (Phyto-Diagnostics, INRA, France) for the presence of Alfalfa mosaic virus (AMV). ELISA results were verified using reverse transcription-polymerase chain reaction (RT-PCR). Total RNA extracts from symptomatic plants were analyzed using primers designed specifically for the coat protein region of AMV utilizing sequence data from GenBank Accession No. AF215664: AMVcoat-F: GT GGT GGG AAA GCT GGT AAA and AMVcoat-R: CAC CCA GTG GAG GTC AGC ATT. The thermocycling schedule was as follows: reverse transcriptase step at 50°C for 30 min, first PCR cycle at 94°C for 2 min, 35 cycles each of 30 s at 94°C, 30 s at 54°C, 30 s at 72°C, followed by a final extension at 72°C for 10 min. A 700-pb PCR product of the expected size was obtained from plants that were positive for AMV using ELISA. The two systems provide for rapid detection of AMV in L. officinalis. A regular screening program will assist in providing virus-free plants to ornamental nurseries. These results demonstrate the presence of AMV in L. officinalis. Alfalfa (Medicago sativa L.) is a typical source of AMV. However, because the nurseries where L. officinalis is grown are not in the vicinity of alfalfa fields, we suggest the source of the infection originated in the propagation material. AMV has currently been reported in L. officinalis only in Italy and France (1). To our knowledge, this is the first report of AMV in L. officinalis in Spain. Reference: (1): A. Garibaldi et al. Ed. Edagricole-Edisioni Agricole della Calderini s.r.l., Bologna, 2000.

First Report of Powdery Mildew of Myosotis sylvatica (Wood Forget-me-not) Caused by Golovinomyces cynoglossi (Erysiphe cynoglossi) in Washington State

2004 ◽  
Vol 5 (1) ◽  
pp. 18
Author(s):  
Dean A. Glawe
Keyword(s):  
Powdery Mildew ◽  
Pacific Northwest ◽  
Washington State ◽  
Causal Organism ◽  
First Report ◽  
The Pacific ◽  
Western Washington ◽  
Symptoms And Signs

Wood forget-me-not cultivars are popular ornamentals in the Pacific Northwest. In western Washington, this species frequently displays symptoms and signs of powdery mildew after anthesis. This report records the disease from King, Pierce, Snohomish, and Skagit counties and describes and illustrates symptoms of the disease as well as taxonomically important features of the causal organism. Accepted for publication 29 October 2004. Published 24 November 2004.

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