Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in rice calli

2014 ◽  
Vol 159 (12) ◽  
pp. 3219-3230 ◽  
Author(s):  
Myunghwan Jung ◽  
Yun Ji Shin ◽  
Ju Kim ◽  
Seung-Bin Cha ◽  
Won-Jung Lee ◽  
...  
Keyword(s):  
Immune Responses ◽  
Oral Administration ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein ◽  
Rice Calli

scholarly journals Immunogenicity in Swine of Orally Administered Recombinant Lactobacillus plantarum Expressing Classical Swine Fever Virus E2 Protein in Conjunction with Thymosin α-1 as an Adjuvant

2015 ◽  
Vol 81 (11) ◽  
pp. 3745-3752 ◽  
Author(s):  
Yi-Gang Xu ◽  
Xue-Ting Guan ◽  
Zhong-Mei Liu ◽  
Chang-Yong Tian ◽  
Li-Chun Cui
Keyword(s):  
Immune Responses ◽  
Lactobacillus Plantarum ◽  
Classical Swine Fever Virus ◽  
Oral Vaccine ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Economic Losses ◽  
E2 Protein ◽  
Content Type ◽  
Cellular Immune

ABSTRACTClassical swine fever, caused by classical swine fever virus (CSFV), is a highly contagious disease that results in enormous economic losses in pig industries. The E2 protein is one of the main structural proteins of CSFV and is capable of inducing CSFV-neutralizing antibodies and cytotoxic T lymphocyte (CTL) activitiesin vivo. Thymosin α-1 (Tα1), an immune-modifier peptide, plays a very important role in the cellular immune response. In this study, genetically engineeredLactobacillus plantarumbacteria expressing CSFV E2 protein alone (L. plantarum/pYG-E2) and in combination with Tα1 (L. plantarum/pYG-E2-Tα1) were developed, and the immunogenicity of each as an oral vaccine to induce protective immunity against CSFV in pigs was evaluated. The results showed that recombinantL. plantarum/pYG-E2 andL. plantarum/pYG-E2-Tα1 were both able to effectively induce protective immune responses in pigs against CSFV infection by eliciting immunoglobulin A (IgA)-based mucosal, immunoglobulin G (IgG)-based humoral, and CTL-based cellular immune responses via oral vaccination. Significant differences (P< 0.05) in the levels of immune responses were observed betweenL. plantarum/pYG-E2-Tα1 andL. plantarum/pYG-E2, suggesting a better immunogenicity ofL. plantarum/pYG-E2-Tα1 as a result of the Tα1 molecular adjuvant that can enhance immune responsiveness and augment specific lymphocyte functions. Our data suggest that the recombinantLactobacillusmicroecological agent expressing CSFV E2 protein combined with Tα1 as an adjuvant provides a promising strategy for vaccine development against CSFV.

Gold nanoparticles enhance immune responses in mice against recombinant classical swine fever virus E2 protein

2020 ◽  
Vol 42 (7) ◽  
pp. 1169-1180 ◽  
Author(s):  
Yafei Li ◽  
Qianyue Jin ◽  
Peiyang Ding ◽  
Wen Zhou ◽  
Yongxiao Chai ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Immune Responses ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein

Immune responses induced by a BacMam virus expressing the E2 protein of classical swine fever virus in mice

2009 ◽  
Vol 125 (2) ◽  
pp. 145-150 ◽  
Author(s):  
Miao Li ◽  
Yu-Fei Wang ◽  
Yu Wang ◽  
Hui Gao ◽  
Na Li ◽  
...  
Keyword(s):  
Immune Responses ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein

Hypervariable antigenic region 1 of classical swine fever virus E2 protein impacts antibody neutralization

Vaccine ◽  
2016 ◽  
Vol 34 (33) ◽  
pp. 3723-3730 ◽  
Author(s):  
Xun Liao ◽  
Zuohuan Wang ◽  
Tong Cao ◽  
Chao Tong ◽  
Shichao Geng ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Antibody Neutralization ◽  
E2 Protein ◽  
Antigenic Region

Immunogenicity of a lettuce-derived vaccine candidate expressing the E2 protein against classical swine fever virus

2013 ◽  
Vol 113 (3) ◽  
pp. 483-490 ◽  
Author(s):  
Jinn-Chin Yiu ◽  
Cheng-Wei Liu ◽  
Ruei-Yuan Su ◽  
Wan-Jun Lai ◽  
Menq-Jiau Tseng ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Vaccine Candidate ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein

scholarly journals Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Qiang Wei ◽  
Yilin Bai ◽  
Yapeng Song ◽  
Yunchao Liu ◽  
Wei Yu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Classical Swine Fever Virus ◽  
Expression System ◽  
Classical Swine Fever ◽  
Neutralizing Antibody ◽  
Baculovirus Expression System ◽  
Viral Envelope ◽  
Fever Virus ◽  
Complete Protection ◽  
Glycoprotein E2

AbstractClassical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and Erns are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and Erns were expressed using the baculovirus system and their protective immunity in rabbits were tested. Twenty CSFV seronegative rabbits were randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-Erns, or their combination (CSFV-E2 + Erns). Besides, a commercial CSFV vaccine (C-strain) and PBS were used as positive or negative controls, respectively. Four weeks after the second immunization, all the rabbits were challenged with 100 RID50 of CSFV C-strain. High levels of CSFV E2-specific antibody, neutralizing antibody and cellular immune responses to CSFV were elicited in the rabbits inoculated with C-strain, CSFV-E2, and CSFV-E2 + Erns. And the rabbits inoculated with the three vaccines received complete protection against CSFV C-strain. However, no neutralizing antibody was detected in the Erns vaccinated rabbits and the rabbits exhibited fever typical of CSFV, suggesting the Erns alone is not able to induce a protective immune response. Taken together, while the Erns could not confer protection against CSFV, E2 and E2 + Erns could not only elicit humoral and cell-mediated immune responses but also confer complete protection against CSFV C-strain in rabbits.

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