High-yield production of canine parvovirus virus-like particles in a baculovirus expression system

2015 ◽  
Vol 161 (3) ◽  
pp. 705-710 ◽  
Author(s):  
Hongli Jin ◽  
Xiaohong Xia ◽  
Bing Liu ◽  
Yu Fu ◽  
Xianping Chen ◽  
...  
Keyword(s):  
Expression System ◽  
Baculovirus Expression System ◽  
Canine Parvovirus ◽  
High Yield ◽  
Virus Like Particles ◽  
Baculovirus Expression ◽  
Yield Production ◽  
High Yield Production

scholarly journals Enhanced production of Canine parvovirus VP2  protein using improved Baculovirus expression  system  and its immunogenicity

2020 ◽  
Author(s):  
Dao Chang ◽  
Yangkun Liu ◽  
Yangyang Chen ◽  
Xiaomin Hu ◽  
Andrey Burov ◽  
...  
Keyword(s):  
Expression System ◽  
Baculovirus Expression System ◽  
Canine Parvovirus ◽  
Full Length ◽  
Recombinant Viruses ◽  
Vp2 Protein ◽  
Enhanced Production ◽  
Baculovirus Expression ◽  
Yield Production ◽  
High Level

Abstract Background : Canine parvovirus (CPV) is now recognized as a serious threat to dog industry worldwide. Vaccination remains the principal tool to control CPV infection. However, due to low yield, production of VP2 protein of CPV in baculovirus expression system remains challenging. The aim of this study was to increase the VP2 protein production by using a improved baculovirus expression system (Multibac) and evaluate the immunogenicity of the purified VP2 protein in mice. Results: The results showed that CPV VP2 protein was successfully expressed in the improved baculovirus expression system efficiently. A high level of expression of the full length VP2 protein was achieved using our modified system. The recombinant virus carrying two copies of VP2 showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein could react with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody with good reactogenicity. The mice were then immunized with purified full length VP2 protein to evaluate its immunogenicity. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions: Full length CPV VP2 protein was successfully expressed at high level and purified efficiently. And it stimulated mice to produce high level of antibody. The full length VP2 expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection.

scholarly journals Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Dao Chang ◽  
Yangkun Liu ◽  
Yangyang Chen ◽  
Xiaomin Hu ◽  
Andrey Burov ◽  
...  
Keyword(s):  
Expression System ◽  
Baculovirus Expression System ◽  
Canine Parvovirus ◽  
Vp2 Protein ◽  
Baculovirus Expression

High-yield production of a functional bacteriophage lysin with antipneumococcal activity using a plant virus-based expression system

2015 ◽  
Vol 200 ◽  
pp. 10-16 ◽  
Author(s):  
Urtė Starkevič ◽  
Luisa Bortesi ◽  
Marius Virgailis ◽  
Modestas Ružauskas ◽  
Anatoli Giritch ◽  
...  
Keyword(s):  
Plant Virus ◽  
Expression System ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

Vibrio natriegens: An Alternative Expression System for the High-Yield Production of Isotopically Labeled Proteins

Biochemistry ◽  
2019 ◽  
Vol 58 (25) ◽  
pp. 2799-2803 ◽  
Author(s):  
Walter Becker ◽  
Florian Wimberger ◽  
Klaus Zangger
Keyword(s):  
Expression System ◽  
High Yield ◽  
Yield Production ◽  
Alternative Expression ◽  
High Yield Production

Process intensification for high yield production of influenza H1N1 Gag virus-like particles using an inducible HEK-293 stable cell line

Vaccine ◽  
2017 ◽  
Vol 35 (33) ◽  
pp. 4220-4228 ◽  
Author(s):  
Alina Venereo-Sanchez ◽  
Melanie Simoneau ◽  
Stéphane Lanthier ◽  
Parminder Chahal ◽  
Lucie Bourget ◽  
...  
Keyword(s):  
Cell Line ◽  
Process Intensification ◽  
High Yield ◽  
Stable Cell Line ◽  
Hek 293 ◽  
Virus Like Particles ◽  
Yield Production ◽  
High Yield Production ◽  
Influenza H1n1

Production of recombinant new canine parvovirus 2a viral protein 2 in SF9 cells using a baculovirus expression system

2020 ◽  
Vol 44 (3) ◽  
pp. 142-145
Author(s):  
Hyeonhae Choi ◽  
Seyeon Park ◽  
Yoon-Hee Lee ◽  
Ju-Yeon Lee ◽  
Jae Young Song ◽  
...  
Keyword(s):  
Viral Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Canine Parvovirus ◽  
Sf9 Cells ◽  
Baculovirus Expression

High-yield production of authentic human growth hormone using a plant virus-based expression system

2005 ◽  
Vol 3 (6) ◽  
pp. 613-620 ◽  
Author(s):  
Mario Gils ◽  
Romy Kandzia ◽  
Sylvestre Marillonnet ◽  
Victor Klimyuk ◽  
Yuri Gleba
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Plant Virus ◽  
Expression System ◽  
Human Growth ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production
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