Pretensive Shared Reality

Imaginative pretend play is often thought of as the domain of young children, yet adults regularly engage in elaborated, fantastical, social-mediated pretend play. We draw on multiple examples, but focus on Table-Top Role Playing games (TTRPG) - and specifically, the most popular and enduring table-top role-playing games, Dungeons & Dragons (D&D) - as a primary example of such play. We describe imaginative play in adults via the term ‘pretensive shared reality'; Shared Pretensive Reality describes the ability of a group of individuals to employ a range of higher-order cognitive functions to explicitly and implicitly share representations of a bounded fictional reality in predictable and coherent ways, such that this constructed reality may be explored and invented/embellished with shared intentionality in an ad hoc manner. Pretensive Shared Reality facilitates multiple individual and social outcomes, including generating personal and group-level enjoyment or mirth, the creation or maintenance of social groups, or the safe exploration of individual self concepts (such as alternative expression of a players sexual or gender identity). Importantly, Pretensive Shared Reality (both within the specific context of table-top role-playing games, and other instances) are primarily co-operative and co-creative. Our conception links the widespread existence and forms of adult imaginative play to childhood pretense, places it within a developmental and evolutionary context, and argues that pretensive shared realities - which underpin many forms of imaginative culture - are an important topic of study unto themselves, and may be utilized to provide methodological insight into a variety of psychological domains.

Virtual Reality: Confinement and Engulfment; Replacement and Re-placement

This chapter explicates Virtual Reality (VR) as the first instantiation of the post-screen. Specifically, it interrogates VR’s sense of immersion via two vectors in the post-screen’s “forgetting” of screen boundaries – confinement of a viewer’s visual field with restricted viewing devices; and engulfment by being surrounded with large screens. The chapter’s key idea is its alternative expression of VR’s relations of reality as an immersive media form, which it argues shifts from the critical paradigms of replacement to re-placement. Through theoretical critique and readings of various applications of VR, the chapter argues for re-placement as a more ethical and generative space for thinking through VR’s relations of the real. In turn, where and how the actual and the virtual is re-placed informs the very purpose of media itself.

Stellar dynamics in the periodic cube

ABSTRACT We use the problem of dynamical friction within the periodic cube to illustrate the application of perturbation theory in stellar dynamics, testing its predictions against measurements from N-body simulations. Our development is based on the explicitly time-dependent Volterra integral equation for the cube’s linear response, which avoids the subtleties encountered in analyses based on complex frequency. We obtain an expression for the self-consistent response of the cube to steady stirring by an external perturber. From this, we show how to obtain the familiar Chandrasekhar dynamical friction formula and construct an elementary derivation of the Lenard–Balescu equation for the secular quasi-linear evolution of an isolated cube composed of N equal-mass stars. We present an alternative expression for the (real-frequency) van Kampen modes of the cube and show explicitly how to decompose any linear perturbation of the cube into a superposition of such modes.

Modern Hebrew Poetry

Modern Hebrew poetry, written in a language comprehensible only to parts of its audience, the Yiddish speaking masses, emerged at the end of the 19th century and became canonized by the time of the publication of C. N. Bialik’s second book in 1908. The Jewish generation that grew up in Eastern Europe after the 1880s aspired to create in Hebrew, a language of ancient texts and commentary, modern alternative expression that matched the pedigree of the European poetry from the Renaissance on. Some Hebrew poetry was written throughout the ages (medieval, Haskalah, Hibat Zion), but in the absence of a steady linear evolution (of models, forms, and prosody), modern Hebrew poetry was a pioneering project accumulated from the biblical narrative monologue and poetry; the commentary and the dialogical tension of the Talmud; the contribution of the Drasha (sermon) tradition; elements of history, literature, folklore, and theology extolled in Halakhic books written throughout the ages; and from threads adapted from the neighboring Russian or German cultures. Seen in retrospect, a growing chorus of Hebrew poets gave voice to the transition of Jews into general Western culture (in its unique realization in the Middle East), the human condition and landscapes, the political and social realities, and the traumas of Jewish existence and its triumph. Their renaissance at the turn of the centuries laid the foundations for the mature poetry written in the new major literary center in Ottoman and British Mandate Palestine and Israel for a new growing class of Hebrew readers.

Magnaporthe oryzae as an expression host for the production of the unspecific peroxygenase AaeUPO from the basidiomycete Agrocybe aegerita

The filamentous fungus Magnaporthe oryzae has the potential to be developed as an alternative platform organism for the heterologous production of industrially important enzymes. M. oryzae is easy to handle, fast-growing and unlike yeast, posttranslational modifications like N-glycosylations are similar to the human organism. Here, we established M. oryzae as a host for the expression of the unspecific peroxygenase from the basidiomycete Agrocybe aegerita (AaeUPO). UPOs are attractive biocatalysts for selective oxyfunctionalization of non-activated carbon-hydrogen bonds. To improve and simplify the isolation of AaeUPO in M. oryzae, we fused a Magnaporthe signal peptide for protein secretion and set it under control of the strong EF1-promotor. The success of the heterologous production of full-length AaeUPO in M. oryzae and the secretion of the functional enzyme was confirmed by a peroxygenase-specific enzyme assay. These results offer the possibility to establish the filamentous ascomycete M. oryzae as a broad applicable alternative expression system. This is in particular valid for proteins that cannot or not in sufficient yields produced in established systems.

Vector Geometric Algebra in Power Systems: An Updated Formulation of Apparent Power under Non-Sinusoidal Conditions

Traditional electrical power theories and one of their most important concepts—apparent power—are still a source of debate, because they present several flaws that misinterpret the power-transfer and energy-balance phenomena under distorted grid conditions. In recent years, advanced mathematical tools such as geometric algebra (GA) have been introduced to address these issues. However, the application of GA to electrical circuits requires more consensus, improvements and refinement. In this paper, electrical power theories for single-phase systems based on GA were revisited. Several drawbacks and inconsistencies of previous works were identified, and some amendments were introduced. An alternative expression is presented for the electric power in the geometric domain. Its norm is compatible with the traditional apparent power defined as the product of the RMS voltage and current. The use of this expression simplifies calculations such as those required for current decomposition. This proposal is valid even for distorted currents and voltages. Concepts are presented in a simple way so that a strong background on GA is not required. The paper included some examples and experimental results in which measurements from a utility supply were analysed.

Mammalian expression of virus-like particles as a proof of principle for next generation polio vaccines

Global vaccination programs using live-attenuated oral and inactivated polio vaccine (OPV and IPV) have almost eradicated poliovirus (PV) but these vaccines or their production pose significant risk in a polio-free world. Recombinant PV virus-like particles (VLPs), lacking the viral genome, represent safe next-generation vaccines, however their production requires optimisation. Here we present an efficient mammalian expression strategy producing good yields of wild-type PV VLPs for all three serotypes and a thermostabilised variant for PV3. Whilst the wild-type VLPs were predominantly in the non-native C-antigenic form, the thermostabilised PV3 VLPs adopted the native D-antigenic conformation eliciting neutralising antibody titres equivalent to the current IPV and were indistinguishable from natural empty particles by cryo-electron microscopy with a similar stabilising lipidic pocket-factor in the VP1 β-barrel. This factor may not be available in alternative expression systems, which may require synthetic pocket-binding factors. VLPs equivalent to these mammalian expressed thermostabilized particles, represent safer non-infectious vaccine candidates for the post-eradication era.

Paradox of modelling curved faults revisited with general non-hypersingular stress Green’s functions

SUMMARY In a dislocation problem, a paradoxical discordance is known to occur between an original smooth curve and an infinitesimally discretized curve. To solve this paradox, we have investigated a non-hypersingular expression for the integral kernel (called the stress Green’s function) which describes the stress field caused by the displacement discontinuity. We first develop a compact alternative expression of the non-hypersingular stress Green’s function for general 2-D and 3-D infinite homogeneous elastic media. We next compute the stress Green’s functions on a curved fault and revisit the paradox. We find that previously obtained non-hypersingular stress Green’s functions are incorrect for curved faults, and that smooth and infinitesimally segmented faults are equivalent. Their compatibility bridges the gap between analytical methods featuring curved faults and numerical methods using subdivided flat patches.

Production of Brucella melitensis Omp16 protein fused to the human interleukin 2 in Lactococcus lactis MG1363 toward developing a Lactococcus-based vaccine against brucellosis

The use of the food-grade bacterium Lactococcus lactis as a new cell factory is a promising alternative expression system for producing a desired protein. The Omp16-IL2 fusion protein antigen was cloned, expressed, and purified in this study. The Omp16-IL2 fusion gene was designed and cloned in pGH plasmid with appropriate restriction sites and subcloned in pAMJ2008 expression vector digested with the same enzymes. The purified recombinant constructed pAMJ-rOmp-IL2 was introduced into L. lactis subsp. cremoris MG1363 by electrotransformation. Finally, the expression and purification of Omp16-IL2 fusion protein was investigated. This study reports the construction of a recombinant L. lactis expressing the Omp16-IL2 fusion protein as an oral Lactococcus-based vaccine, as compared with commonly used live attenuated vaccines, for future studies against brucellosis.