Molecular characterization of a divergent strain of calla lily chlorotic spot virus infecting celtuce (Lactuca sativa var. augustana) in China

Local chlorotic spots resembling early lesions characteristic of citrus leprosis (CL) were observed in leaves of two sweet orange (Citrus sinensis L.) trees in Teresina, State of Piauí, Brazil, in early 2017. However, despite the similarities, these spots were generally larger than those of a typical CL and showed rare or no necrosis symptoms. In symptomatic tissues, transmission electron microscopy revealed the presence of viroplasms in the nuclei of the infected parenchymal cells and rod-shaped particles with an average size of approximately 40 × 100 nm, resembling those typically observed during infection by dichorhaviruses. A bipartite genome of the putative novel virus, tentatively named citrus chlorotic spot virus (CiCSV) (RNA1 = 6,518 nucleotides [nt] and RNA2 = 5,987 nt), revealed the highest nucleotide sequence identity values with the dichorhaviruses coffee ringspot virus strain Lavras (73.8%), citrus leprosis virus N strain Ibi1 (58.6%), and orchid fleck virus strain So (56.9%). In addition to citrus, CiCSV was also found in local chlorotic lesions on leaves of the ornamental plant beach hibiscus (Talipariti tiliaceum (L.) Fryxell). Morphological characterization of mites recovered from the infected plants revealed at least two different types of Brevipalpus. One of them corresponds to Brevipalpus yothersi. The other is slightly different from B. yothersi mites but comprises traits that possibly place it as another species. A mix of the two mite types collected on beach hibiscus successfully transmitted CiCSV to arabidopsis plants but additional work is required to verify whether both types of flat mite may act as viral vectors. The current study reveals a newly described dichorhavirus associated with a citrus disease in the northeastern region of Brazil.

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Complete genome sequence of a distinct calla lily chlorotic spot virus isolated in mainland China

Archives of Virology ◽

10.1007/s00705-015-2630-9 ◽

2015 ◽

Vol 161 (1) ◽

pp. 219-222 ◽

Cited By ~ 4

Author(s):

Ye Xu ◽

Si-bo Wang ◽

Yong-zhong Li ◽

Hong-zheng Tao ◽

Ya-ning Huang ◽

...

Keyword(s):

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Mainland China ◽

Spot Virus ◽

Chlorotic Spot ◽

Calla Lily

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SEQUENCE ANALYSIS OF S RNA OF CALLA LILY CHLOROTIC SPOT VIRUS, A NEW TOSPOVIRUS

Acta Horticulturae ◽

10.17660/actahortic.2006.722.13 ◽

2006 ◽

pp. 103-110

Author(s):

Y.H. Lin ◽

T.C. Chen ◽

M.H. Chung ◽

C.C. Chen ◽

S.D. Yeh ◽

...

Keyword(s):

Sequence Analysis ◽

Spot Virus ◽

Chlorotic Spot ◽

Calla Lily

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Serological Comparison and Molecular Characterization for Verification of Calla lily chlorotic spot virus as a New Tospovirus Species Belonging to Watermelon silver mottle virus Serogroup

Phytopathology ◽

10.1094/phyto-95-1482 ◽

2005 ◽

Vol 95 (12) ◽

pp. 1482-1488 ◽

Cited By ~ 38

Author(s):

Yu-Hsuan Lin ◽

Tsung-Chi Chen ◽

Hei-Ti Hsu ◽

Fang-Lin Liu ◽

Fang-Hua Chu ◽

...

Keyword(s):

Open Reading Frames ◽

Mottle Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Spot Virus ◽

Heterologous Antigen ◽

Chlorotic Spot ◽

Calla Lily ◽

Homologous Antigen ◽

Distinct Member ◽

Watermelon Silver Mottle Virus

Calla lily chlorotic spot virus (CCSV) isolated from central Taiwan was recently identified as a tospovirus serologically but distantly related to Watermelon silver mottle virus (WSMoV). To clarify the serological relationship between the two viruses, rabbit polyclonal antibody (PAb) to CCSV and mouse monoclonal antibodies (MAbs) to WSMoV NP or CCSV NP were produced in this investigation, using purified nucleocapsid protein (NP) as immunogens. The PAb to CCSV NP reacted stronger with the homologous antigen than with the heterologous antigen, with much lower A405 readings in indirect enzyme-linked immunosorbent assay (ELISA) and low-intensity banding in immunoblotting. MAbs produced to CCSV NP or WSMoV NP reacted specifically with the homologous antigens but not with the heterologous antigens in both ELISA and immunoblot analyses. The CCSV S RNA was determined to be 3,172 nucleotides in length, with an inverted repeat at the 5′ and 3′ ends and two open reading frames encoding the NP and a nonstructural (NSs) protein in an ambisense arrangement. A typical 3′-terminal sequence (5′-AUUGCUCU-3′) that is shared by all members of the genus Tospovirus also is present in the CCSV S RNA. The CCSV NP and NSs protein share low amino acid identities of 20.1 to 65.1% and 19.9 to 66.1%, respectively, with those of reported tospoviruses. Phylogenetic dendrogram analysis indicates that CCSV is a distinct member in the genus Tospovirus. The results provide evidence that CCSV is a new species in the genus Tospovirus and belongs to WSMoV serogroup.

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First Report of Capsicum chlorosis virus Causing Yellow Stripes on Calla Lilies in Taiwan

Plant Disease ◽

10.1094/pdis-91-9-1201c ◽

2007 ◽

Vol 91 (9) ◽

pp. 1201-1201 ◽

Cited By ~ 9

Author(s):

C. C. Chen ◽

C. H. Huang ◽

T. C. Chen ◽

S. D. Yeh ◽

Y. H. Cheng ◽

...

Keyword(s):

Chenopodium Quinoa ◽

Amino Acid Sequences ◽

N Gene ◽

Impatiens Necrotic Spot Virus ◽

Leaf Extracts ◽

Spot Virus ◽

Chlorotic Spot ◽

Calla Lily ◽

Capsicum Chlorosis Virus ◽

Virus Isolates

Tomato spotted wilt virus (TSWV) and Calla lily chlorotic spot virus (CCSV) are two recognized species of the Tospovirus genus in the family Bunyaviridae infecting calla lily (Zantedeschia spp.). During 2005, 15 virus isolates were collected from different calla lily plants exhibiting yellow stripes on their leaves in Ho-Li, a major calla lily-production township in Taiwan. After three successive local lesion passages on Chenopodium quinoa Willd., diseased leaf tissues individually infected by these isolates were preserved in liquid nitrogen and used for subsequent identification studies. Using the tospovirus genus-specific primers gL3637 and gL4435c designed from the L RNA, an 800-bp DNA fragment was amplified in reverse transcription-PCR from all 15 isolates. Moreover, leaf extracts of the diseased calla lilies and the C. quinoa plants inoculated with the 15 virus isolates reacted with antisera against the nucleocapsid proteins (NP) of Capsicum chlorosis virus (CaCV)-gloxinia and Watermelon silver mottle virus (WSMoV), but not to monoclonal antibodies against the NP of TSWV, CCSV, Peanut chlorotic fan-spot virus (PCFV), or Impatiens necrotic spot virus (INSV) in indirect ELISA. These results indicate that the 15 virus isolates are tospoviruses belonging to the WSMoV serogroup. Additionally, we amplified and sequenced the full-length N gene from these tospovirus isolates using primers WN2328 (5′-CCATTGGTTTGCCTCCG-3′) and WN3534 (5′-CGTCGACAGAGCAATCGAGGC-3′) designed from the S RNA of WSMoV. The deduced amino acid sequences of the N protein from these 15 tospovirus isolates showed a greater than 92% identity to that of CaCV (GenBank Accession No. NC-008301). Furthermore, results of phylogenetic analysis of the 15 isolates on the basis of amino acids sequences, both genetic distance and parsimony trees indicated that they were all genetically clustered within CaCV using INSV, TSWV, and WSMoV as outgroups. The results indicate that the virus causing yellow stripes in calla lilies is a strain of CaCV. To our knowledge, this is the first evidence that CaCV can naturally infect calla lilies and cause yellow stripe symptoms. Reference: (1) F.-H. Chu et al. Phytopathology 91:361, 2001.

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Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2007000900016 ◽

2007 ◽

Vol 42 (9) ◽

pp. 1335-1343 ◽

Cited By ~ 16

Author(s):

Renata Faier Calegario ◽

Sávio de Siqueira Ferreira ◽

Eduardo Chumbinho de Andrade ◽

Francisco Murilo Zerbini

Keyword(s):

Host Range ◽

Molecular Characterization ◽

Phylogenetic Analyses ◽

Datura Stramonium ◽

Sweet Pepper ◽

Yellow Spot ◽

Spot Virus ◽

Sequence Comparisons ◽

Full Length Genome

The objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment as inoculation methods. The yellow spot virus can infect plants in Solanaceae and Amaranthaceae, including economically importat crops as sweet pepper, and weeds as Datura stramonium and Nicotiana silvestris. For the molecular characterization, the full-length genome (DNA-A and DNA-B) was amplified, cloned and completely sequenced. Sequence comparisons and phylogenetic analyses indicated that TGV-[Bi2] constitutes a novel begomovirus species named Tomato yellow spot virus (ToYSV), closely related to Sida mottle virus (SiMoV).

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