Clinical evaluation of an in-house-developed real-time RT-PCR assay for serotyping of dengue virus

2020 ◽  
Vol 165 (10) ◽  
pp. 2311-2315 ◽  
Author(s):  
M. B. Kakade ◽  
N. Shrivastava ◽  
J. A. Patil ◽  
D. Parashar ◽  
P. S. Shah ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Clinical Evaluation ◽  
Rt Pcr ◽  
Pcr Assay

Detection of dengue virus serotypes by single-tube multiplex RT-PCR and multiplex real-time PCR assay

2021 ◽  
Author(s):  
Kundan Tandel ◽  
Mahadevan Kumar ◽  
G.S. Bhalla ◽  
S.P.S. Shergill ◽  
Vijaya Swarnim ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Dengue Virus Serotypes

scholarly journals O486 Implementation of a real-time RT-PCR assay to improve diagnostics of dengue virus infections

2007 ◽  
Vol 29 ◽  
pp. S104-S105
Author(s):  
A. Dumoulin ◽  
H.P. Marti ◽  
M. Panning ◽  
H.H. Hirsch
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Virus Infections ◽  
Rt Pcr ◽  
Pcr Assay

scholarly journals Spatio-temporal distribution analysis of circulating genotypes of dengue virus type 1 in western and southern states of India by a one-step real-time RT-PCR assay

2019 ◽  
Vol 75 ◽  
pp. 103989 ◽  
Author(s):  
K. Alagarasu ◽  
J.A. Patil ◽  
M.B. Kakade ◽  
A.M. More ◽  
M. Bote ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Temporal Distribution ◽  
Distribution Analysis ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Southern States ◽  
One Step ◽  
Spatio Temporal

A two-tube multiplex real-time RT-PCR assay for the detection of four hemorrhagic fever viruses: severe fever with thrombocytopenia syndrome virus, Hantaan virus, Seoul virus, and dengue virus

2013 ◽  
Vol 158 (9) ◽  
pp. 1857-1863 ◽  
Author(s):  
Zhifeng Li ◽  
Xian Qi ◽  
Minghao Zhou ◽  
Changjun Bao ◽  
Jianli Hu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Hemorrhagic Fever ◽  
Hantaan Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Seoul Virus ◽  
Severe Fever

scholarly journals Individual-based dengue virus surveillance in Aedes aegypti mosquitoes collected concurrently with suspected patients in Tarlac City, Philippines

2020 ◽  
Author(s):  
Jean Claude Balingit ◽  
Thaddeus M. Carvajal ◽  
Mariko Saito-Obata ◽  
Maribet Gamboa ◽  
Amalea Dulcene Nicolasora ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Vector Control ◽  
Real Time ◽  
Phylogenetic Analyses ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Virus Surveillance ◽  
One Step ◽  
Mosquito Infection

Abstract Background: Vector control measures are critical in the prevention and reduction of dengue virus (DENV) transmission. In this context, effective vector control is reliant not only on knowledge of mosquito abundance, but also on the timely and accurate detection of mosquito infection. Mosquito-based virus surveillance programs commonly rely on pool-based mosquito testing, but whether individual-based mosquito testing could represent a feasible alternative is not largely studied. Applying an individual-based mosquito testing approach, we conducted a one-month DENV surveillance of adult Aedes aegypti mosquitoes around households of suspected dengue patients during the 2015 dengue peak season in Tarlac City, Philippines to more accurately assess the mosquito infection rate, and to identify the DENV serotypes and genotypes concurrently co-circulating in mosquitoes and patients. Methods: We performed a one-step multiplex real-time RT-PCR assay for the simultaneous detection and serotyping of DENV in patients and in individual female Ae. aegypti mosquito. Additionally, we performed sequencing and phylogenetic analyses to further characterize the detected DENVs in mosquitoes and patients at the genotype level. Results: We collected a total of 583 adult Ae. aegypti mosquitoes, of which we tested 359 female mosquitoes individually for the presence of the DENV. Ten mosquitoes (2.8%) from amongst 359 female mosquitoes were confirmed to be positive for the presence of the DENV. We detected DENV-1, DENV-2, and DENV-4 in the field-collected mosquitoes, which were consistent with the serotypes concurrently infecting patients. Sequencing and phylogenetic analyses of the detected DENVs based on the partial envelope (E) gene revealed three genotypes concurrently present in the sampled mosquitoes and patients during the study period, namely: DENV-1 genotype IV, DENV-2 Cosmopolitan genotype and DENV-4 genotype II. Conclusions: In this study, we demonstrate the utility of a one-step multiplex real-time RT-PCR assay in individual-based DENV surveillance of mosquitoes. Our findings reinforce the importance of detecting and monitoring virus activity in local mosquito populations, which is critical for dengue prevention and control activities.

scholarly journals Clinical evaluation of a multiplex real-time RT-PCR assay for detection of SARS-CoV-2 in individual and pooled upper respiratory tract samples

2021 ◽  
Author(s):  
Melissa Laverack ◽  
Rebecca L. Tallmadge ◽  
Roopa Venugopalan ◽  
Brittany Cronk ◽  
XiuLin Zhang ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Real Time ◽  
Clinical Evaluation ◽  
Upper Respiratory Tract ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Upper Respiratory

scholarly journals Validation of an internally controlled one-step real-time multiplex RT-PCR assay for the detection and quantitation of dengue virus RNA in plasma

2011 ◽  
Vol 177 (2) ◽  
pp. 168-173 ◽  
Author(s):  
Kien Duong Thi Hue ◽  
Trung Vu Tuan ◽  
Hanh Tien Nguyen Thi ◽  
Chau Tran Nguyen Bich ◽  
Huy Huynh Le Anh ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Virus Rna ◽  
One Step

scholarly journals Correction: Analytical and Clinical Performance of the CDC Real Time RT-PCR Assay for Detection and Typing of Dengue Virus

2013 ◽  
Vol 7 (7) ◽  
Author(s):  
Gilberto A. Santiago ◽  
Edgardo Vergne ◽  
Yashira Quiles ◽  
Joan Cosme ◽  
Jesus Vazquez ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Clinical Performance ◽  
Rt Pcr ◽  
Pcr Assay

scholarly journals Analytical and Clinical Performance of the CDC Real Time RT-PCR Assay for Detection and Typing of Dengue Virus

2013 ◽  
Vol 7 (7) ◽  
pp. e2311 ◽  
Author(s):  
Gilberto A. Santiago ◽  
Edgardo Vergne ◽  
Yashira Quiles ◽  
Joan Cosme ◽  
Jesus Vazquez ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Clinical Performance ◽  
Rt Pcr ◽  
Pcr Assay

scholarly journals Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Jean Claude Balingit ◽  
Thaddeus M. Carvajal ◽  
Mariko Saito-Obata ◽  
Maribet Gamboa ◽  
Amalea Dulcene Nicolasora ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Vector Control ◽  
Real Time ◽  
Phylogenetic Analyses ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Denv Serotypes ◽  
One Step

Abstract Background Vector control measures are critical for the prevention and reduction of dengue virus (DENV) transmission. Effective vector control is reliant not only on knowledge of mosquito abundance, but also on the timely and accurate detection of mosquito-borne infection. Mosquito-based virus surveillance programs typically rely on pool-based mosquito testing, although whether individual-based mosquito testing is a feasible alternative to this has not been widely studied. Applying an individual-based mosquito testing approach, we conducted a 1-month surveillance study of DENV in adult Aedes aegypti mosquitoes in homes of suspected dengue patients during the 2015 peak dengue season in Tarlac City, Philippines to more accurately assess the mosquito infection rate and identify the DENV serotypes and genotypes concurrently co-circulating in mosquitoes and patients there. Methods We performed a one-step multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection and serotyping of DENV in patients and individual female Ae. aegypti mosquitoes. Additionally, we performed sequencing and phylogenetic analyses to further characterize the detected DENV serotypes in mosquitoes and patients at the genotype level. Results We collected a total of 583 adult Ae. aegypti mosquitoes, of which we individually tested 359 female mosquitoes for the presence of DENV. Ten (2.8%) of the 359 female mosquitoes were positive for the presence of DENV. We detected DENV-1, DENV-2, and DENV-4 in the field-collected mosquitoes, which was consistent with the serotypes concurrently found in infected patients. Sequencing and phylogenetic analyses of the detected DENV serotypes based on the partial sequence of the evelope (E) gene revealed three genotypes concurrently present in the sampled mosquitoes and patients during the study period, namely DENV-1 genotype IV, DENV-2 Cosmopolitan genotype, and DENV-4 genotype II. Conclusions We demonstrated the utility of a one-step multiplex real-time RT-PCR assay for the individual-based DENV surveillance of mosquitoes. Our findings reinforce the importance of detecting and monitoring virus activity in local mosquito populations, which are critical for dengue prevention and control.

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